MKX Antibody - #DF2430

Fig. 9. The controlled release of TPCA-1 promotes tenogenic differentiation of hPDLSCs for tendon repair in rats. (A) H&E staining and MT staining of the repaired tendons in the Ctrl or TPCA-1 groups. Black arrows indicated the cells with the characteristics of inflammatory cells at 4 weeks. Low magnification: scale bars = 1 mm; high magnification: scale bars = 40 μm. (B) Total histology scores of repaired tendons in the Ctrl and TPCA-1 groups from H&E staining images at 4 weeks after injury (n = 8 biological replicates). A lower score indicates a more mature tendon tissue. (C) Quantitative analysis of collagen density in the repaired tendons of the Ctrl and TPCA-1 groups from MT staining images (n = 8 biological replicates). (D–E) Representative images of IF staining for SCX (D) and TNMD (E) in the repaired tendons of the Ctrl and TPCA-1 groups at 4 weeks after injury. Scale bars = 50 μm. (F–G) Quantitative analysis of IF staining for SCX (F) and TNMD (G). (H) Representative images and quantitative analysis of IHC staining for MKX at 4 weeks after injury. Scale bars = 20 μm. (I) Quantitative analysis of intensity parameters via gait analysis at 2 weeks after injury (n = 5 biological replicates). (J) Typical strain-stress curves of repaired tendons. (K) Tensile modulus, max stress, and max force of repaired tendons at 4 weeks after injury (n = 5 biological replicates). Results were shown as means ± SEM. n = 6 randomly-selected microscopic images for quantitative analysis for Fig. 9F–H. P values were determined by the Student's t-test (D–F) and Mann-Whiteney test (G). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.