ZFPM1 Antibody - #DF2428

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Product: ZFPM1 Antibody
Catalog: DF2428
Description: Rabbit polyclonal antibody to ZFPM1
Application: WB
Reactivity: Human, Mouse
Prediction: Pig
Mol.Wt.: 104 kDa; 105kD(Calculated).
Uniprot: Q8IX07
RRID: AB_2839634

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(80%)
Clonality:
Polyclonal
Specificity:
ZFPM1 Antibody detects endogenous levels of total ZFPM1.
RRID:
AB_2839634
Cite Format: Affinity Biosciences Cat# DF2428, RRID:AB_2839634.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FOG-1; FOG1_HUMAN; Friend of GATA 1; Friend of GATA protein 1; Friend of GATA1; ZFPM1; Zinc finger protein; Zinc finger protein 89A; Zinc finger protein multitype 1; Zinc finger protein ZFPM1;

Immunogens

Immunogen:
Uniprot:

Q8IX07(FOG1_HUMAN) >>Visit HPA database.

Gene(ID):
ZFPM1(161882)
Expression:
Q8IX07 FOG1_HUMAN:

Mainly expressed in hematopoietic tissues. Also expressed in adult cerebellum, stomach, lymph node, liver and pancreas. Expressed in fetal heart, liver and spleen.

Description:
Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2.
Sequence:
MSRRKQSNPRQIKRSLGDMEAREEVQLVGASHMEQKATAPEAPSPPSADVNSPPPLPPPTSPGGPKELEGQEPEPRPTEEEPGSPWSGPDELEPVVQDGQRRIRARLSLATGLSWGPFHGSVQTRASSPRQAEPSPALTLLLVDEACWLRTLPQALTEAEANTEIHRKDDALWCRVTKPVPAGGLLSVLLTAEPHSTPGHPVKKEPAEPTCPAPAHDLQLLPQQAGMASILATAVINKDVFPCKDCGIWYRSERNLQAHLLYYCASRQGTGSPAAAATDEKPKETYPNERVCPFPQCRKSCPSASSLEIHMRSHSGERPFVCLICLSAFTTKANCERHLKVHTDTLSGVCHSCGFISTTRDILYSHLVTNHMVCQPGSKGEIYSPGAGHPATKLPPDSLGSFQQQHTALQGPLASADLGLAPTPSPGLDRKALAEATNGEARAEPLAQNGGSSEPPAAPRSIKVEAVEEPEAAPILGPGEPGPQAPSRTPSPRSPAPARVKAELSSPTPGSSPVPGELGLAGALFLPQYVFGPDAAPPASEILAKMSELVHSRLQQGAGAGAGGAQTGLFPGAPKGATCFECEITFSNVNNYYVHKRLYCSGRRAPEDAPAARRPKAPPGPARAPPGQPAEPDAPRSSPGPGAREEGAGGAATPEDGAGGRGSEGSQSPGSSVDDAEDDPSRTLCEACNIRFSRHETYTVHKRYYCASRHDPPPRRPAAPPGPPGPAAPPAPSPAAPVRTRRRRKLYELHAAGAPPPPPPGHAPAPESPRPGSGSGSGPGLAPARSPGPAADGPIDLSKKPRRPLPGAPAPALADYHECTACRVSFHSLEAYLAHKKYSCPAAPPPGALGLPAAACPYCPPNGPVRGDLLEHFRLAHGLLLGAPLAGPGVEARTPADRGPSPAPAPAASPQPGSRGPRDGLGPEPQEPPPGPPPSPAAAPEAVPPPPAPPSYSDKGVQTPSKGTPAPLPNGNHRYCRLCNIKFSSLSTFIAHKKYYCSSHAAEHVK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
80
Xenopus
64
Bovine
55
Zebrafish
55
Horse
0
Sheep
0
Dog
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IX07 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation
S44 Phosphorylation
S47 Phosphorylation
S52 Phosphorylation
S84 Phosphorylation
S87 Phosphorylation
Y262 Phosphorylation
S266 Phosphorylation
T270 Phosphorylation
S272 Phosphorylation
T278 Phosphorylation
Y383 Phosphorylation
S384 Phosphorylation
K463 Sumoylation
S487 Phosphorylation
T489 Phosphorylation
S491 Phosphorylation
S494 Phosphorylation
K501 Sumoylation
S638 Phosphorylation
T653 Phosphorylation
S666 Phosphorylation
S668 Phosphorylation
S671 Phosphorylation
S681 Phosphorylation
Y698 Phosphorylation
T699 Phosphorylation
Y704 Phosphorylation
S708 Phosphorylation
S733 Phosphorylation
S768 Phosphorylation
S773 Phosphorylation
S786 Phosphorylation
S798 Phosphorylation
T894 Phosphorylation
S901 Phosphorylation
S909 Phosphorylation
S914 Phosphorylation
S935 Phosphorylation

Research Backgrounds

Function:

Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2 (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mainly expressed in hematopoietic tissues. Also expressed in adult cerebellum, stomach, lymph node, liver and pancreas. Expressed in fetal heart, liver and spleen.

Subunit Structure:

Interacts with corepressor CTBP2; this interaction is however not essential for corepressor activity (By similarity). Interacts with the N-terminal zinc-finger of GATA1, GATA2 and probably GATA3.

Family&Domains:

The CCHC FOG-type zinc fingers 1, 2, 3 and 5 directly bind to GATA-type zinc fingers. The Tyr residue adjacent to the last Cys of the CCHC FOG-type zinc finger is essential for the interaction with GATA-type zinc fingers (By similarity).

Belongs to the FOG (Friend of GATA) family.

Restrictive clause

 

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