RB6I2 Antibody - #DF2426

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Product: RB6I2 Antibody
Catalog: DF2426
Description: Rabbit polyclonal antibody to RB6I2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 128kDa; 128kD(Calculated).
Uniprot: Q8IUD2
RRID: AB_2839632

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%)
Clonality:
Polyclonal
Specificity:
RB6I2 Antibody detects endogenous levels of total RB6I2.
RRID:
AB_2839632
Cite Format: Affinity Biosciences Cat# DF2426, RRID:AB_2839632.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cast 2; CAST2; CAZ associated structural protein 2; Elks; ELKS/RAB6 interacting/CAST family member 1; ELKS/RAB6-interacting/CAST family member 1; ERC 1; ERC protein 1; ERC-1; Erc1; ERC1 protein; KIAA1081; MGC12974; RAB6 interacting protein 2; RAB6-interacting protein 2; RAB6IP2; RB6I2_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q8IUD2(RB6I2_HUMAN) >>Visit HPA database.

Gene(ID):
ERC1(23085)
Expression:
Q8IUD2 RB6I2_HUMAN:

Widely expressed. Isoform 2 and isoform 4 are abundantly expressed in brain. Isoform 1 and isoform 3 are predominantly expressed in testis and thyroid, and isoform 1 predominates in other tissues tested.

Sequence:
MYGSARSVGKVEPSSQSPGRSPRLPRSPRLGHRRTNSTGGSSGSSVGGGSGKTLSMENIQSLNAAYATSGPMYLSDHENVGSETPKSTMTLGRSGGRLPYGVRMTAMGSSPNIASSGVASDTIAFGEHHLPPVSMASTVPHSLRQARDNTIMDLQTQLKEVLRENDLLRKDVEVKESKLSSSMNSIKTFWSPELKKERALRKDEASKITIWKEQYRVVQEENQHMQMTIQALQDELRIQRDLNQLFQQDSSSRTGEPCVAELTEENFQRLHAEHERQAKELFLLRKTLEEMELRIETQKQTLNARDESIKKLLEMLQSKGLSAKATEEDHERTRRLAEAEMHVHHLESLLEQKEKENSMLREEMHRRFENAPDSAKTKALQTVIEMKDSKISSMERGLRDLEEEIQMLKSNGALSTEEREEEMKQMEVYRSHSKFMKNKVEQLKEELSSKEAQWEELKKKAAGLQAEIGQVKQELSRKDTELLALQTKLETLTNQFSDSKQHIEVLKESLTAKEQRAAILQTEVDALRLRLEEKETMLNKKTKQIQDMAEEKGTQAGEIHDLKDMLDVKERKVNVLQKKIENLQEQLRDKEKQMSSLKERVKSLQADTTNTDTALTTLEEALAEKERTIERLKEQRDRDEREKQEEIDNYKKDLKDLKEKVSLLQGDLSEKEASLLDLKEHASSLASSGLKKDSRLKTLEIALEQKKEECLKMESQLKKAHEAALEARASPEMSDRIQHLEREITRYKDESSKAQAEVDRLLEILKEVENEKNDKDKKIAELERQVKDQNKKVANLKHKEQVEKKKSAQMLEEARRREDNLNDSSQQLQDSLRKKDDRIEELEEALRESVQITAEREMVLAQEESARTNAEKQVEELLMAMEKVKQELESMKAKLSSTQQSLAEKETHLTNLRAERRKHLEEVLEMKQEALLAAISEKDANIALLELSSSKKKTQEEVAALKREKDRLVQQLKQQTQNRMKLMADNYEDDHFKSSHSNQTNHKPSPDQIIQPLLELDQNRSKLKLYIGHLTTLCHDRDPLILRGLTPPASYNLDDDQAAWENELQKMTRGQLQDELEKGERDNAELQEFANAILQQIADHCPDILEQVVNALEESS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
90
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IUD2 As Substrate

Site PTM Type Enzyme
Y2 Phosphorylation
S4 Phosphorylation
R6 Methylation
S7 Phosphorylation
K10 Acetylation
S14 Phosphorylation
S15 Phosphorylation
S17 Phosphorylation
R20 Methylation
S21 Phosphorylation
S27 Phosphorylation
T35 Phosphorylation
S37 Phosphorylation
T38 Phosphorylation
S41 Phosphorylation
T53 Phosphorylation
S55 Phosphorylation
Y73 Phosphorylation
S75 Phosphorylation
R93 Methylation
S94 Phosphorylation
S181 Phosphorylation
S191 Phosphorylation
S250 Phosphorylation
C258 S-Nitrosylation
K299 Ubiquitination
S374 Phosphorylation
K488 Ubiquitination
K552 Ubiquitination
K590 Acetylation
K602 Ubiquitination
S603 Phosphorylation
K625 Ubiquitination
K652 Acetylation
K655 Acetylation
K658 Acetylation
K671 Ubiquitination
S674 Phosphorylation
K679 Ubiquitination
S688 Phosphorylation
K691 Ubiquitination
K719 Ubiquitination
S730 Phosphorylation
R736 Methylation
K766 Ubiquitination
K799 Acetylation
K805 Acetylation
S807 Phosphorylation
S824 Phosphorylation
K835 Ubiquitination
K894 Ubiquitination
S901 Phosphorylation
K905 Ubiquitination
K973 Ubiquitination
Y987 Phosphorylation
K1003 Ubiquitination
S1005 Phosphorylation
T1046 Phosphorylation
S1050 Phosphorylation
Y1051 Phosphorylation
K1066 Ubiquitination

Research Backgrounds

Function:

Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm. Membrane>Peripheral membrane protein. Golgi apparatus membrane>Peripheral membrane protein. Cell junction>Synapse>Presynaptic cell membrane.
Note: Recruited on Golgi membranes by RAB6A in a GTP-dependent manner (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Isoform 2 and isoform 4 are abundantly expressed in brain. Isoform 1 and isoform 3 are predominantly expressed in testis and thyroid, and isoform 1 predominates in other tissues tested.

Subunit Structure:

Part of a complex with CHUK, IKBKB and IKBKG. Interacts with CHUK, IKBKB and IKBKG. The interaction with IKBKG is independent of CHUK and IKBKB. Interacts with NFKBIA. Isoform 4 interacts with PPFIA1, and through its C-terminus with the PDZ domains of RIMS1 and RIMS2. Interacts with ERC2/CAST1. Interacts with the GTB-bound forms of RAB6A isoform 1 and isoform 2 and with RAB6B. The interaction was strongest with RAB6B, followed by RAB6A isoform 2 and weakest with RAB6A isoform 1 (By similarity). Interacts with SDCCAG8.

Research Fields

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

References

1). ZnO NPs induce miR-342-5p mediated ferroptosis of spermatocytes through the NF-κB pathway in mice. JOURNAL OF NANOBIOTECHNOLOGY, 2024 [IF=10.8]

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