TTC19 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using TTC19 Antibody.

DF2412 at 1/100 staining Rat heart tissue by IHC-P.

Product:	TTC19 Antibody
Catalog:	DF2412
Description:	Rabbit polyclonal antibody to TTC19
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	35 kDa; 42kD(Calculated).
Uniprot:	Q6DKK2
RRID:	AB_2839618

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

TTC19 Antibody detects endogenous levels of total TTC19.

RRID:

AB_2839618
Cite Format: Affinity Biosciences Cat# DF2412, RRID:AB_2839618.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2010204O13Rik; FLJ20343; MGC138312; MGC19520; mitochondrial; RIKEN cDNA 2810460C24; Tetratricopeptide repeat protein 19; TPR repeat protein 19; Ttc 19; TTC19; TTC19_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q6DKK2(TTC19_HUMAN) >>Visit HPA database.

Gene(ID):

TTC19(54902)

Description:

Mitochondrial protein required for formation of the mitochondrial complex III (PubMed:21278747). May also be required for the abcission step in cytokinesis, possibly regulating the ESCRT-III complex via its interaction with CHMP4B (PubMed:20208530). However, the involvement in cytokinesis requires additional experimental evidence.

Sequence:

Q6DKK2 TTC19_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFRLLSWSLGRGFLRAAGRRCRGCSARLLPGLAGGPGPEVQVPPSRVAPHGRGPGLLPLLAALAWFSRPAAAEEEEQQGADGAAAEDGADEAEAEIIQLLKRAKLSIMKDEPEEAELILHDALRLAYQTDNKKAITYTYDLMANLAFIRGQLENAEQLFKATMSYLLGGGMKQEDNAIIEISLKLASIYAAQNRQEFAVAGYEFCISTLEEKIEREKELAEDIMSVEEKANTHLLLGMCLDACARYLLFSKQPSQAQRMYEKALQISEEIQGERHPQTIVLMSDLATTLDAQGRFDEAYIYMQRASDLARQINHPELHMVLSNLAAVLMHRERYTQAKEIYQEALKQAKLKKDEISVQHIREELAELSKKSRPLTNSVKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6DKK2 As Substrate

Q6DKK2

Site	PTM Type	Source
K101	Ubiquitination	Uniprot
K132	Ubiquitination	Uniprot
S225	Phosphorylation	Uniprot
K251	Ubiquitination	Uniprot
K262	Ubiquitination	Uniprot
K346	Ubiquitination	Uniprot
K352	Ubiquitination	Uniprot
K370	Ubiquitination	Uniprot
S377	Phosphorylation	Uniprot

Research Backgrounds

Q6DKK2_TTC19_HUMAN

Function:

Required for the preservation of the structural and functional integrity of mitochondrial respiratory complex III by allowing the physiological turnover of the Rieske protein UQCRFS1. Involved in the clearance of UQCRFS1 N-terminal fragments, which are produced upon incorporation of UQCRFS1 into the complex III and whose presence is detrimental for its catalytic activity.

Subcellular Location:

Mitochondrion inner membrane.

Subunit Structure:

Binds to the mature mitochondrial complex III dimer, after the incorporation of the Rieske protein UQCRFS1. Interacts with UQCRC1 and UQCRFS1 (By similarity). Interacts with ZFYVE26 and CHMP4B.

Family&Domains:

Belongs to the TTC19 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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TTC19 Antibody - #DF2412