TAF7L Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/3

Western blot analysis of extracts from 293 cells, using TAF7L Antibody.

Figure 2 Generation of Taf7lD144G knockin mice.

Product:	TAF7L Antibody
Catalog:	DF2403
Description:	Rabbit polyclonal antibody to TAF7L
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Sheep, Dog
Mol.Wt.:	42 kDa; 53kD(Calculated).
Uniprot:	Q5H9L4
RRID:	AB_2839610

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MSDS

Data Sheet

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Protocols

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IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%), Bovine(88%), Sheep(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

TAF7L Antibody detects endogenous levels of total TAF7L.

RRID:

AB_2839610
Cite Format: Affinity Biosciences Cat# DF2403, RRID:AB_2839610.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cancer/testis antigen 40; CT40; FLJ23157; RNA polymerase II TBP-associated factor subunit Q; TAF2Q; TAF7 like RNA polymerase II, TATA box binding protein (TBP) associated factor; Taf7l; TAF7L_HUMAN; TATA box binding protein-associated factor, RNA polymerase II, Q; TATA box-binding protein-associated factor 50 kDa; TBP associated factor, RNA polymerase II, Q; Transcription initiation factor TFIID 50 kDa subunit; Transcription initiation factor TFIID subunit 7-like;

Immunogens

Immunogen:

Uniprot:

Q5H9L4(TAF7L_HUMAN) >>Visit HPA database.

Gene(ID):

TAF7L(54457)

Expression:

Q5H9L4 TAF7L_HUMAN:

Testis-specific.

Description:

Probably functions as a spermatogensis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis.

Sequence:

Q5H9L4 TAF7L_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MECPEGQLPISSENDSTPTVSTSEVTSQQEPQILVDRGSETTYESSADIAGDEGTQIPADEDTQTDADSSAQAAAQAPENFQEGKDMSESQDEVPDEVENQFILRLPLEHACTVRNLARSQSVKMKDKLKIDLLPDGRHAVVEVEDVPLAAKLVDLPCVIESLRTLDKKTFYKTADISQMLVCTADGDIHLSPEEPAASTDPNIVRKKERGREEKCVWKHGITPPLKNVRKKRFRKTQKKVPDVKEMEKSSFTEYIESPDVENEVKRLLRSDAEAVSTRWEVIAEDGTKEIESQGSIPGFLISSGMSSHKQGHTSSEYDMLREMFSDSRSNNDDDEDEDDEDEDEDEDEDEDEDKEEEEEDCSEEYLERQLQAEFIESGQYRANEGTSSIVMEIQKQIEKKEKKLHKIQNKAQRQKDLIMKVENLTLKNHFQSVLEQLELQEKQKNEKLISLQEQLQRFLKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Dog

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5H9L4 As Substrate

Q5H9L4

Site	PTM Type	Source
T184	Phosphorylation	Uniprot
T223	Phosphorylation	Uniprot
S258	Phosphorylation	Uniprot

Research Backgrounds

Q5H9L4_TAF7L_HUMAN

Function:

Probably functions as a spermatogenesis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis (By similarity).

Subcellular Location:

Nucleus. Cytoplasm.
Note: Cytoplasmic in spermatogonia and early spermatocytes (preleptotene, leptotene, and zygotene); translocates into the nuclei of pachytene spermatocytes and round spermatids.

Tissue Specificity:

Testis-specific.

Subunit Structure:

TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TAF7L may replace TAF7 in a spermatogenesis-specific form of TFIID. Interacts with TBP; the interaction occurs in a sub-population of cells (pachytene and haploid round spermatids) and is developmentally regulated through differential intracellular localization of the two proteins. Interacts with TAF1 (By similarity).

Family&Domains:

Belongs to the TAF7 family.

Research Fields

· Genetic Information Processing > Transcription > Basal transcription factors.

References

1). Genetic characterization of a missense mutation in the X-linked TAF7L gene identified in an oligozoospermic man. BIOLOGY OF REPRODUCTION, 2022 (PubMed: 35554494) [IF=3.6]

Application: WB Species: Mouse Sample:

Figure 2 Generation of Taf7lD144G knockin mice. (A) Schematic of mouse Taf7l gene structure, targeting vector, and various alleles. Taf7l consists of 13 exons. Only the first nine exons are shown in rectangles. Exons 10 to 13 are not shown. The asterisk in Exon 4 denotes the point mutation. PGK-Neo encodes a neomycin selection marker. HyTK enables positive selection with hygromycin (not used) and negative selection with ganciclovir. pOG231 expresses Cre recombinase. (B) Genotyping of Taf7l alleles. (C) Sequencing of the wild type and Taf7lD144G alleles. (D) qRT-PCR analysis of the Taf7l transcript in wild type and Taf7lD144G/Y testes from 3-month-old mice. n.s.: nonsignificant, by Student’s t-test. (E) Western blot analysis of TAF7L in 3-month-old wild type and Taf7lD144G/Y testes. GAPDH serves as a loading control.

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TAF7L Antibody - #DF2403