TAF1B Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

Western blot analysis of extracts from various samples, using TAF1B Antibody.

Western blot analysis of extracts from Mouse kidney, using TAF1B Antibody.

DF2402 at 1/100 staining Rat brain tissue by IHC-P.

DF2402 staining HepG2 by IF/ICC.

Product:	TAF1B Antibody
Catalog:	DF2402
Description:	Rabbit polyclonal antibody to TAF1B
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	69 kDa; 69kD(Calculated).
Uniprot:	Q53T94
RRID:	AB_2839609

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

TAF1B Antibody detects endogenous levels of total TAF1B.

RRID:

AB_2839609
Cite Format: Affinity Biosciences Cat# DF2402, RRID:AB_2839609.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MGC:9349; RAF1B; RAFI63; SL1; TAB1 B; TAF1B TATA box binding protein (TBP) associated factor, RNA polymerase I, B, 63kDa; TAFI63; TATA box binding protein associated factor 1B; TATA box binding protein associated factor RNA polymerase I subunit B;

Immunogens

Immunogen:

Uniprot:

Q53T94(TAF1B_HUMAN) >>Visit HPA database.

Gene(ID):

TAF1B(9014)

Sequence:

Q53T94 TAF1B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDLEEAEEFKERCTQCAAVSWGLTDEGKYYCTSCHNVTERYQEVTNTDLIPNTQIKALNRGLKKKNNTEKGWDWYVCEGFQYILYQQAEALKNLGVGPELKNDVLHNFWKRYLQKSKQAYCKNPVYTTGRKPTVLEDNLSHSDWASEPELLSDVSCPPFLESGAESQSDIHTRKPFPVSKASQSETSVCSGSLDGVEYSQRKEKGIVKMTMPQTLAFCYLSLLWQREAITLSDLLRFVEEDHIPYINAFQHFPEQMKLYGRDRGIFGIESWPDYEDIYKKTVEVGTFLDLPRFPDITEDCYLHPNILCMKYLMEVNLPDEMHSLTCHVVKMTGMGEVDFLTFDPIAKMAKTVKYDVQAVAIIVVVLKLLFLLDDSFEWSLSNLAEKHNEKNKKDKPWFDFRKWYQIMKKAFDEKKQKWEEARAKYLWKSEKPLYYSFVDKPVAYKKREMVVNLQKQFSTLVESTATAGKKSPSSFQFNWTEEDTDRTCFHGHSLQGVLKEKGQSLLTKNSLYWLSTQKFCRCYCTHVTTYEESNYSLSYQFILNLFSFLLRIKTSLLHEEVSLVEKKLFEKKYSVKRKKSRSKKVRRH

PTMs - Q53T94 As Substrate

Q53T94

Site	PTM Type	Source
	Acetylation	Uniprot
M1	Acetylation	Uniprot
K56	Ubiquitination	Uniprot
K122	Ubiquitination	Uniprot
K174	Ubiquitination	Uniprot
K395	Ubiquitination	Uniprot
Y425	Phosphorylation	Uniprot
K431	Ubiquitination	Uniprot
Y434	Phosphorylation	Uniprot
Y435	Phosphorylation	Uniprot
K440	Acetylation	Uniprot
K440	Ubiquitination	Uniprot
K501	Ubiquitination	Uniprot
K508	Ubiquitination	Uniprot

Research Backgrounds

Q53T94_TAF1B_HUMAN

Function:

Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3.

Subcellular Location:

Nucleus>Nucleolus.

Subunit Structure:

Interacts with FLNA (via N-terminus) (By similarity). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B, TAF1C and TAF1D. In the complex interacts directly with TBP, TAF1A and TAF1C. Interaction of the SL1/TIF-IB subunits with TBP excludes interaction of TBP with the transcription factor IID (TFIID) subunits. Interacts with TBP and RRN3.

Family&Domains:

Although it shares weak sequence similarity with GTF2B/TFIIB, displays a similar subdomain organization as GTF2B/TFIIB, with a N-terminal zinc finger, a connecting region (composed of B-reader and B-linker regions), followed by 2 cyclin folds. The RRN7-type zinc finger plays an essential postrecruitment role in Pol I transcription at a step preceding synthesis of the first 40 nucleotides (PubMed:21921198 and PubMed:21921199).

Belongs to the RRN7/TAF1B family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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TAF1B Antibody - #DF2402