EIF4G2 Antibody - #DF2379

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Product: EIF4G2 Antibody
Catalog: DF2379
Description: Rabbit polyclonal antibody to EIF4G2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 102 kDa; 102kD(Calculated).
Uniprot: P78344
RRID: AB_2839587

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
EIF4G2 Antibody detects endogenous levels of total EIF4G2.
RRID:
AB_2839587
Cite Format: Affinity Biosciences Cat# DF2379, RRID:AB_2839587.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DAP-5; Death associated protein 5; Death-associated protein 5; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; EIF4G2; Eukaryotic translation initiation factor 4 gamma 2; IF4G2_HUMAN; Nat1; Novel APOBEC-1 target 1; p97; Translation repressor NAT1; Translation repressor NAT1;

Immunogens

Immunogen:
Uniprot:

P78344(IF4G2_HUMAN) >>Visit HPA database.

Gene(ID):
EIF4G2(1982)
Expression:
P78344 IF4G2_HUMAN:

Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.

Description:
Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
Sequence:
MESAIAEGGASRFSASSGGGGSRGAPQHYPKTAGNSEFLGKTPGQNAQKWIPARSTRRDDNSAANNSANEKERHDAIFRKVRGILNKLTPEKFDKLCLELLNVGVESKLILKGVILLIVDKALEEPKYSSLYAQLCLRLAEDAPNFDGPAAEGQPGQKQSTTFRRLLISKLQDEFENRTRNVDVYDKRENPLLPEEEEQRAIAKIKMLGNIKFIGELGKLDLIHESILHKCIKTLLEKKKRVQLKDMGEDLECLCQIMRTVGPRLDHERAKSLMDQYFARMCSLMLSKELPARIRFLLQDTVELREHHWVPRKAFLDNGPKTINQIRQDAVKDLGVFIPAPMAQGMRSDFFLEGPFMPPRMKMDRDPLGGLADMFGQMPGSGIGTGPGVIQDRFSPTMGRHRSNQLFNGHGGHIMPPTQSQFGEMGGKFMKSQGLSQLYHNQSQGLLSQLQGQSKDMPPRFSKKGQLNADEISLRPAQSFLMNKNQVPKLQPQITMIPPSAQPPRTQTPPLGQTPQLGLKTNPPLIQEKPAKTSKKPPPSKEELLKLTETVVTEYLNSGNANEAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATSDNFMQAFLNVLDQCPKLEVDIPLVKSYLAQFAARAIISELVSISELAQPLESGTHFPLFLLCLQQLAKLQDREWLTELFQQSKVNMQKMLPEIDQNKDRMLEILEGKGLSFLFPLLKLEKELLKQIKLDPSPQTIYKWIKDNISPKLHVDKGFVNILMTSFLQYISSEVNPPSDETDSSSAPSKEQLEQEKQLLLSFKPVMQKFLHDHVDLQVSALYALQVHCYNSNFPKGMLLRFFVHFYDMEIIEEEAFLAWKEDITQEFPGKGKALFQVNQWLTWLETAEEEESEEEAD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78344 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S3 Phosphorylation
S11 Phosphorylation
S14 Phosphorylation
S16 Phosphorylation
S17 Phosphorylation
S22 Phosphorylation
R23 Methylation
K31 Methylation
K31 Ubiquitination
K41 Methylation
K41 Ubiquitination
T42 Phosphorylation
K49 Ubiquitination
K71 Ubiquitination
K87 Ubiquitination
T89 Phosphorylation
K92 Ubiquitination
K95 Ubiquitination
K158 Ubiquitination
K170 Ubiquitination
Y185 Phosphorylation
K187 Ubiquitination
K204 Ubiquitination
K206 Ubiquitination
K212 Ubiquitination
K219 Ubiquitination
S226 Phosphorylation
K230 Acetylation
K230 Ubiquitination
K233 Acetylation
K233 Ubiquitination
K245 Ubiquitination
K271 Ubiquitination
K288 Ubiquitination
K313 Ubiquitination
K321 Ubiquitination
K332 Ubiquitination
R360 Methylation
K362 Ubiquitination
S381 Phosphorylation
T385 Phosphorylation
R393 Methylation
S395 Phosphorylation
T397 Phosphorylation
R400 Methylation
R402 Methylation
K431 Methylation
K431 Ubiquitination
S432 Phosphorylation
S436 Phosphorylation
Y439 Phosphorylation
S443 Phosphorylation
S448 Phosphorylation
S454 Phosphorylation
R460 Methylation
K463 Methylation
K464 Methylation
K464 Ubiquitination
S473 Phosphorylation
R475 Methylation
S479 Phosphorylation
K484 Ubiquitination
S500 Phosphorylation
R505 Methylation
T506 Phosphorylation
T508 Phosphorylation
T514 Phosphorylation
K520 Ubiquitination
K529 Acetylation
K529 Ubiquitination
K541 Ubiquitination
K546 Ubiquitination
K575 Ubiquitination
K584 Ubiquitination
R592 Methylation
K599 Ubiquitination
S602 Phosphorylation
S605 Phosphorylation
K640 Ubiquitination
S641 Phosphorylation
Y642 Phosphorylation
R687 Methylation
K698 Ubiquitination
K703 Ubiquitination
K712 Ubiquitination
K732 Ubiquitination
K735 Ubiquitination
K739 Ubiquitination
K742 Ubiquitination
Y751 Phosphorylation
K755 Ubiquitination
S759 Phosphorylation
K761 Ubiquitination
K806 Ubiquitination
S811 Phosphorylation
K813 Ubiquitination
K880 Ubiquitination
S902 Phosphorylation

Research Backgrounds

Function:

Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.

PTMs:

Phosphorylation; hyperphosphorylated during mitosis.

Tissue Specificity:

Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.

Subunit Structure:

Interacts with the serine/threonine protein kinases MKNK1 and MKNK2. Binds EIF4A and EIF3. Interacts with MIF4GD.

Family&Domains:

Belongs to the eukaryotic initiation factor 4G family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

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