GADD45B Antibody - #DF2375

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Product: GADD45B Antibody
Catalog: DF2375
Description: Rabbit polyclonal antibody to GADD45B
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 18kDa,22kDa; 18kD(Calculated).
Uniprot: O75293
RRID: AB_2839583

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
GADD45B Antibody detects endogenous levels of total GADD45B.
RRID:
AB_2839583
Cite Format: Affinity Biosciences Cat# DF2375, RRID:AB_2839583.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GA45B_HUMAN; GADD45 Beta; Gadd45b; GADD45BETA; Growth arrest and DNA damage inducible 45 beta; Growth arrest and DNA damage inducible beta; Growth arrest and DNA damage-inducible gene GADD45, beta; Growth arrest and DNA damage-inducible protein GADD45 beta; Growth arrest and DNA-damage-inducible protein GADD45 beta; MY118; MYD118; MYD118, mouse, homolog of; Myeloid differentiation primary response gene; Myeloid differentiation primary response protein MyD118; Negative growth regulatory protein MyD118; Negative growth-regulatory protein MyD118;

Immunogens

Immunogen:
Uniprot:

O75293(GA45B_HUMAN) >>Visit HPA database.

Gene(ID):
GADD45B(4616)
Description:
Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.
Sequence:
MTLEELVACDNAAQKMQTVTAAVEELLVAAQRQDRLTVGVYESAKLMNVDPDSVVLCLLAIDEEEEDDIALQIHFTLIQSFCCDNDINIVRVSGMQRLAQLLGEPAETQGTTEARDLHCLLVTNPHTDAWKSHGLVEVASYCEESRGNNQWVPYISLQER

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
78
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75293 As Substrate

Site PTM Type Enzyme
T2 Phosphorylation

Research Backgrounds

Function:

Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.

Subunit Structure:

Interacts with GADD45GIP1.

Family&Domains:

Belongs to the GADD45 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

References

1). GADD45B Facilitates Metastasis of Ovarian Cancer Through Epithelial–Mesenchymal Transition. OncoTargets and Therapy (PubMed: 33469306) [IF=4.0]

Application: WB    Species: Human    Sample: EOC cell

Figure 3 GADD45B knockdown inhibited the migration of EOC cells. (A, B) Western blot and qRT-PCR detected the expression of GADD45B in the EOC cell lines. (C, D) qRT-PCR and Western blot analysis of the silencing efficiency of GADD45B. (E, G) Wound-healing assays detected the migration ability difference of ES2 and SKOV3 cells between the NC group and the GADD45B siRNA-downregulated groups. (F, H) Histograms show the wound-healing areas of the scratch-wound assays. (I) Transwell assays detected the migration ability of ES2 and SKOV3 cells in the NC group and the GADD45B siRNA-downregulated groups. (J, K) Histograms show the number of migrating cells. Experiments were performed at least in triplicate, and the results are shown as the mean ± s.d. Student’s t-test was used to analyse the data (*p < 0.05; **p < 0.01; ***p < 0.001). Scale bar, 100 μm.

Application: IHC    Species: Human    Sample: tumour tissues

Figure 7 GADD45B is overexpressed in omental metastatic tissues of EOC over matched primary tumour tissues. (A) Representative images of the immunohistochemical analysis of GADD45B in one of the paired tumour tissues. Scale bar, 20 μm. (B) Statistical result of the mean density of GADD45B in 11 pairs of matched EOC tissue samples. Paired Student’s t-test was used to analyse the data (**p < 0.01).

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