MAP4K5 Antibody - #DF2337

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Product: MAP4K5 Antibody
Catalog: DF2337
Description: Rabbit polyclonal antibody to MAP4K5
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 95 kDa; 95kD(Calculated).
Uniprot: Q9Y4K4
RRID: AB_2839561

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MAP4K5 Antibody detects endogenous levels of total MAP4K5.
RRID:
AB_2839561
Cite Format: Affinity Biosciences Cat# DF2337, RRID:AB_2839561.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 2.7.11.1; KHS; Kinase homologous to SPS1/STE20; MAPK/ERK kinase kinase kinase 5; MEK kinase kinase 5; MEKKK 5;

Immunogens

Immunogen:
Uniprot:

Q9Y4K4(M4K5_HUMAN) >>Visit HPA database.

Gene(ID):
MAP4K5(11183)
Expression:
Q9Y4K4 M4K5_HUMAN:

Ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate.

Sequence:
MEAPLRPAADILRRNPQQDYELVQRVGSGTYGDVYKARNVHTGELAAVKIIKLEPGDDFSLIQQEIFMVKECKHCNIVAYFGSYLSREKLWICMEYCGGGSLQDIYHVTGPLSELQIAYVCRETLQGLAYLHTKGKMHRDIKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIELGELQPPMFDLHPMRALFLMSKSNFQPPKLKDKTKWSSTFHNFVKIALTKNPKKRPTAERLLTHTFVAQPGLSRALAVELLDKVNNPDNHAHYTEADDDDFEPHAIIRHTIRSTNRNARAERTASEINFDKLQFEPPLRKETEARDEMGLSSDPNFMLQWNPFVDGANTGKSTSKRAIPPPLPPKPRISSYPEDNFPDEEKASTIKHCPDSESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQLPRKKDKRDFPKPAINGLPPTPKVLMGACFSKVFDGCPLKINCATSWIHPDTKDQYIIFGTEDGIYTLNLNELHEATMEQLFPRKCTWLYVINNTLMSLSVGKTFQLYSHNLIALFEHAKKPGLAAHIQTHRFPDRILPRKFALTTKIPDTKGCHKCCIVRNPYTGHKYLCGALQSGIVLLQWYEPMQKFMLIKHFDFPLPSPLNVFEMLVIPEQEYPMVCVAISKGTESNQVVQFETINLNSASSWFTEIGAGSQQLDSIHVTQLERDTVLVCLDKFVKIVNLQGKLKSSKKLASELSFDFRIESVVCLQDSVLAFWKHGMQGKSFKSDEVTQEISDETRVFRLLGSDRVVVLESRPTENPTAHSNLYILAGHENSY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
88
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y4K4 As Substrate

Site PTM Type Enzyme
Y20 Phosphorylation
T30 Phosphorylation
Y31 Phosphorylation
Y35 Phosphorylation
K36 Ubiquitination
K142 Ubiquitination
T168 Phosphorylation
K171 Ubiquitination
S174 Phosphorylation
T178 Phosphorylation
T267 Phosphorylation
Y303 Phosphorylation
T304 Phosphorylation
T320 Phosphorylation
T333 Phosphorylation
S335 Phosphorylation
K341 Ubiquitination
T379 Phosphorylation
S399 Phosphorylation
S400 Phosphorylation
Y401 Phosphorylation
K411 Ubiquitination
S421 Phosphorylation
S433 Phosphorylation
S434 Phosphorylation
S436 Phosphorylation
S445 Phosphorylation
K500 Ubiquitination
K508 Ubiquitination
K589 Ubiquitination
K609 Ubiquitination
K615 Ubiquitination
K624 Ubiquitination
K748 Ubiquitination
K755 Ubiquitination
K793 Ubiquitination
K796 Ubiquitination
S816 Phosphorylation
S845 Phosphorylation

PTMs - Q9Y4K4 As Enzyme

Substrate Site Source
P49841 (GSK3B) S9 Uniprot

Research Backgrounds

Function:

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate.

Subunit Structure:

Interacts with both SH3 domains of the adapter proteins CRK and CRKL.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Restrictive clause

 

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