JIP4 Antibody - #DF2333

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Product: JIP4 Antibody
Catalog: DF2333
Description: Rabbit polyclonal antibody to JIP4
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 156.8 kDa,; 146kD(Calculated).
Uniprot: O60271
RRID: AB_2839557

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
JIP4 Antibody detects endogenous levels of total JIP4.
RRID:
AB_2839557
Cite Format: Affinity Biosciences Cat# DF2333, RRID:AB_2839557.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C jun amino terminal kinase interacting protein 4; c Jun NH2 terminal kinase associated leucine zipper protein; FLJ13450; FLJ14006; FLJ34602; HLC 4; HLC 4 protein; HLC 6; HLC4; HLC4 protein; HLC6; HSS; Human lung cancer protein 6; JIP 4; JIP4; JNK associated leucine zipper protein 1; JNK associated leucine zipper protein; JNK interacting protein 4; JNK interacting protein; JNK/SAPK associated protein; KIAA0516; Lung cancer oncogene 4; MAPK8IP4; Max binding protein; MGC117291; MGC14967; MGC74461; Mitogen activated protein kinase 8 interacting protein 4; PHET; PIG 6; PIG6; Proliferation inducing gene 6; Protein highly expressed in testis; SPAG 9; SPAG9; Sperm associated antigen 9; Sperm specific protein; Sperm surface protein; Sunday driver (drosophila) homolog; Sunday driver 1; SYD 1; SYD1;

Immunogens

Immunogen:
Uniprot:

O60271(JIP4_HUMAN) >>Visit HPA database.

Gene(ID):
SPAG9(9043)
Expression:
O60271 JIP4_HUMAN:

Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis. Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients.

Sequence:
MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
92
Chicken
91
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60271 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y9 Phosphorylation
K102 Ubiquitination
S109 Phosphorylation
S123 Phosphorylation
K134 Sumoylation
S143 Phosphorylation
K154 Ubiquitination
K178 Ubiquitination
S183 Phosphorylation
S185 Phosphorylation
S190 Phosphorylation
S194 Phosphorylation
S203 Phosphorylation
T217 Phosphorylation
T226 Phosphorylation
S229 Phosphorylation
K233 Ubiquitination
S238 Phosphorylation
S242 Phosphorylation
T244 Phosphorylation
S245 Phosphorylation
S249 Phosphorylation
S251 Phosphorylation
S265 Phosphorylation
S268 Phosphorylation
S272 Phosphorylation
T275 Phosphorylation
T276 Phosphorylation
S279 Phosphorylation
S283 Phosphorylation
T290 Phosphorylation
T292 Phosphorylation
S311 Phosphorylation
S314 Phosphorylation
S329 Phosphorylation
T330 Phosphorylation
S332 Phosphorylation
S339 Phosphorylation
S347 Phosphorylation
T348 Phosphorylation
S358 Phosphorylation
Y360 Phosphorylation
K361 Ubiquitination
S363 Phosphorylation
S364 Phosphorylation
T365 Phosphorylation
T367 Phosphorylation
S387 Phosphorylation
S388 Phosphorylation
S391 Phosphorylation
T418 Phosphorylation
K445 Ubiquitination
R474 Methylation
S493 Phosphorylation
S538 Phosphorylation
S561 Phosphorylation
K577 Methylation
K577 Ubiquitination
Y578 Phosphorylation
T582 Phosphorylation
T586 Phosphorylation P24941 (CDK2)
S588 Phosphorylation
S593 Phosphorylation
S594 Phosphorylation
T595 Phosphorylation
S597 Phosphorylation
K603 Ubiquitination
K605 Ubiquitination
S620 Phosphorylation
K653 Ubiquitination
Y654 Phosphorylation
K699 Ubiquitination
S705 Phosphorylation
Y713 Phosphorylation
S728 Phosphorylation
S730 Phosphorylation
S732 Phosphorylation
S733 Phosphorylation
K742 Acetylation
K746 Acetylation
K749 Acetylation
S813 Phosphorylation
S815 Phosphorylation
Y914 Phosphorylation
S949 Phosphorylation
K998 Ubiquitination
S1144 Phosphorylation
S1188 Phosphorylation
K1227 Ubiquitination
S1238 Phosphorylation
T1264 Phosphorylation
S1290 Phosphorylation
S1302 Phosphorylation
K1305 Ubiquitination

Research Backgrounds

Function:

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg-interaction. Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex.

PTMs:

Phosphorylated by MAPK8 and MAPK14.

Subcellular Location:

Cytoplasm. Cytoplasm>Perinuclear region. Lysosome membrane.
Note: Perinuclear distribution in response to stress signals such as UV radiation.

Cytoplasmic vesicle>Secretory vesicle>Acrosome.
Note: Associated with the plasma membrane of the acrosomal compartment and also localizes in the acrosome matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis. Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients.

Subunit Structure:

Homodimer. The homodimer interacts with ARF6, forming a heterotetramer. Homooligomer. Interacts with MAX, MAPK14, MAP3K3, MYC, KNS2 and MAP2K4 (By similarity). Interaction with KNS2 is important in the formation of ternary complex with MAPK8 (By similarity). Interacts with NFKB1. Isoform 5 interacts with MAPK8, MAPK9, MAPK10. Interacts with PIP4P1.

Family&Domains:

Belongs to the JIP scaffold family.

Restrictive clause

 

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For Research Use Only.
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