CENPJ Antibody - #DF2312

(2)  
Product: CENPJ Antibody
Catalog: DF2312
Description: Rabbit polyclonal antibody to CENPJ
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 149kDa; 153kD(Calculated).
Uniprot: Q9HC77
RRID: AB_2839536

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
CENPJ Antibody detects endogenous levels of total CENPJ.
RRID:
AB_2839536
Cite Format: Affinity Biosciences Cat# DF2312, RRID:AB_2839536.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BM032; CENP-J; CENPJ; CENPJ_HUMAN; Centromere protein J; Centrosomal P4.1-associated protein; CPAP; LAG-3-associated protein; LAP; LIP1; LYST interacting protein LIP1; LYST interacting protein LIP7; LYST-interacting protein 1; MCPH6; Sas 4; SASS4; SCKL4;

Immunogens

Immunogen:
Uniprot:

Q9HC77(CENPJ_HUMAN) >>Visit HPA database.

Gene(ID):
CENPJ(55835)
Description:
Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome
Sequence:
MFLMPTSSELNSGQNFLTQWMTNPSRAGVILNRGFPILEADKEKRAAVDISTSFPIKGTHFSDSFSFINEEDSLLEEQKLESNNPYKPQSDKSETHTAFPCIKKGPQVAACHSAPGHQEENKNDFIPDLASEFKEGAYKDPLFKKLEQLKEVQQKKQEQLKRQQLEQLQRLMEEQEKLLTMVSGQCTLPGLSLLPDDQSQKHRSPGNTTTGERATCCFPSYVYPDPTQEETYPSNILSHEQSNFCRTAHGDFVLTSKRASPNLFSEAQYQEAPVEKNNLKEENRNHPTGESILCWEKVTEQIQEANDKNLQKHDDSSEVANIEERPIKAAIGERKQTFEDYLEEQIQLEEQELKQKQLKEAEGPLPIKAKPKQPFLKRGEGLARFTNAKSKFQKGKESKLVTNQSTSEDQPLFKMDRQQLQRKTALKNKELCADNPILKKDSKARTKSGSVTLSQKPKMLKCSNRKSLSPSGLKIQTGKKCDGQFRDQIKFENKVTSNNKENVTECPKPCDTGCTGWNKTQGKDRLPLSTGPASRLAAKSPIRETMKESESSLDVSLQKKLETWEREKEKENLELDEFLFLEQAADEISFSSNSSFVLKILERDQQICKGHRMSSTPVKAVPQKTNPADPISHCNRSEDLDHTAREKESECEVAPKQLHSLSSADELREQPCKIRKAVQKSTSENQTEWNARDDEGVPNSDSSTDSEEQLDVTIKPSTEDRERGISSREDSPQVCDDKGPFKDTRTQEDKRRDVDLDLSDKDYSSDESIMESIKHKVSEPSRSSSLSLSKMDFDDERTWTDLEENLCNHDVVLGNESTYGTPQTCYPNNEIGILDKTIKRKIAPVKRGEDLSKSRRSRSPPTSELMMKFFPSLKPKPKSDSHLGNELKLNISQDQPPGDNARSQVLREKIIELETEIEKFKAENASLAKLRIERESALEKLRKEIADFEQQKAKELARIEEFKKEEMRKLQKERKVFEKYTTAARTFPDKKEREEIQTLKQQIADLREDLKRKETKWSSTHSRLRSQIQMLVRENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQVEKYKKNYLPMQGNPPRRSKSAPPRDLGNLDKGQAASPREPLEPLNFPDPEYKEEEEDQDIQGEISHPDGKVEKVYKNGCRVILFPNGTRKEVSADGKTITVTFFNGDVKQVMPDQRVIYYYAAAQTTHTTYPEGLEVLHFSSGQIEKHYPDGRKEITFPDQTVKNLFPDGQEESIFPDGTIVRVQRDGNKLIEFNNGQRELHTAQFKRREYPDGTVKTVYANGHQETKYRSGRIRVKDKEGNVLMDTEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
92
Zebrafish
83
Chicken
83
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HC77 As Substrate

Site PTM Type Enzyme
S53 Phosphorylation
K103 Ubiquitination
S113 Phosphorylation
K134 Ubiquitination
K150 Ubiquitination
K201 Ubiquitination
K257 Ubiquitination
S260 Phosphorylation
S265 Phosphorylation
Y269 Phosphorylation
K297 Ubiquitination
K308 Ubiquitination
S316 Phosphorylation
K328 Ubiquitination
T337 Phosphorylation
Y341 Phosphorylation
K399 Ubiquitination
S405 Phosphorylation
S448 Phosphorylation
T452 Phosphorylation
K466 Acetylation
S467 Phosphorylation O14965 (AURKA)
S469 Phosphorylation
S471 Phosphorylation
S497 Phosphorylation
K508 Ubiquitination
K519 Ubiquitination
K539 Ubiquitination
S540 Phosphorylation
S551 Phosphorylation
S556 Phosphorylation
K560 Ubiquitination
S589 Phosphorylation Q9NYY3 (PLK2)
S595 Phosphorylation O00444 (PLK4) , Q9NYY3 (PLK2)
S614 Phosphorylation
S615 Phosphorylation
T616 Phosphorylation
K624 Ubiquitination
T625 Phosphorylation
T643 Phosphorylation
K656 Ubiquitination
S663 Phosphorylation
K680 Ubiquitination
S681 Phosphorylation
T682 Phosphorylation
S683 Phosphorylation
T704 Phosphorylation
S717 Phosphorylation
S731 Phosphorylation
S759 Phosphorylation
S764 Phosphorylation
S765 Phosphorylation
S768 Phosphorylation
K776 Acetylation
S781 Phosphorylation
S783 Phosphorylation
S787 Phosphorylation
K790 Acetylation
T798 Phosphorylation
T800 Phosphorylation
S857 Phosphorylation
S859 Phosphorylation
T862 Phosphorylation
K888 Ubiquitination
S892 Phosphorylation
K929 Ubiquitination
K979 Ubiquitination
K1000 Ubiquitination
S1081 Phosphorylation
S1109 Phosphorylation
S1125 Phosphorylation
K1253 Ubiquitination
K1296 Ubiquitination

Research Backgrounds

Function:

Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Involved in the regulation of slow processive growth of centriolar microtubules. Acts as microtubule plus-end tracking protein that stabilizes centriolar microtubules and inhibits microtubule polymerization and extension from the distal ends of centrioles. Required for centriole elongation and for STIL-mediated centriole amplification. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner. May be involved in the control of centriolar-microtubule growth by acting as a regulator of tubulin release.

PTMs:

Phosphorylation at Ser-589 and Ser-595 by PLK2 is required for procentriole formation and centriole elongation. Phosphorylation by PLK2 oscillates during the cell cycle: it increases at G1/S transition and decreases during the exit from mitosis. Phosphorylation at Ser-595 is also mediated by PLK4 but is not a critical step in PLK4 function in procentriole assembly.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.
Note: Localized within the center of microtubule asters (PubMed:11003675). During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles (PubMed:17681131).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms homodimers. Associates with microtubules plus ends; binds to beta-tublin subunits exposed on microtubule outer surface at its distal tip; also associates with microtubule lattice. Associated with the gamma-tubulin complex. Interacts with the head domain of EPB41. Interacts with LYST. Interacts with CEP152 (via C-terminus). Interacts with STIL. Forms a complex with STIL and SASS6.

Family&Domains:

Belongs to the TCP10 family.

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