CENPI Antibody | Affinity Biosciences

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Western blot analysis of CENPI expression in Hela cell lysates.

DF2311 at 1/100 staining Human gastric tissue by IHC-P.

Product:	CENPI Antibody
Catalog:	DF2311
Description:	Rabbit polyclonal antibody to CENPI
Application:	WB IHC
Reactivity:	Human
Mol.Wt.:	87 kDa; 87kD(Calculated).
Uniprot:	Q92674
RRID:	AB_2839535

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

CENPI Antibody detects endogenous levels of total CENPI.

RRID:

AB_2839535
Cite Format: Affinity Biosciences Cat# DF2311, RRID:AB_2839535.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CENP I; centromere protein I; Follicle simulating hormone primary response protein; FSH primary response 1; FSH primary response protein 1; FSH primary response, rat, homolog of, 1; FSHP1; FSHPRH1; interphase centromere complex protein 19; Leucine rich primary response gene 1; Leucine rich primary response protein 1; LRPR1; Mis6;

Immunogens

Immunogen:

Uniprot:

Q92674(CENPI_HUMAN) >>Visit HPA database.

Gene(ID):

CENPI(2491)

Sequence:

Q92674 CENPI_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSPQKRVKNVQAQNRTSQGSSSFQTTLSAWKVKQDPSNSKNISKHGQNNPVGDYEHADDQAEEDALQMAVGYFEKGPIKASQNKDKTLEKHLKTVENVAWKNGLASEEIDILLNIALSGKFGNAVNTRILKCMIPATVISEDSVVKAVSWLCVGKCSGSTKVLFYRWLVAMFDFIDRKEQINLLYGFFFASLQDDALCPYVCHLLYLLTKKENVKPFRVRKLLDLQAKMGMQPHLQALLSLYKFFAPALISVSLPVRKKIYFKNSENLWKTALLAVKQRNRGPSPEPLKLMLGPANVRPLKRKWNSLSVIPVLNSSSYTKECGKKEMSLSDCLNRSGSFPLEQLQSFPQLLQNIHCLELPSQMGSVLNNSLLLHYINCVRDEPVLLRFYYWLSQTLQEECIWYKVNNYEHGKEFTNFLDTIIRAECFLQEGFYSCEAFLYKSLPLWDGLCCRSQFLQLVSWIPFSSFSEVKPLLFDHLAQLFFTSTIYFKCSVLQSLKELLQNWLLWLSMDIHMKPVTNSPLETTLGGSMNSVSKLIHYVGWLSTTAMRLESNNTFLLHFILDFYEKVCDIYINYNLPLVVLFPPGIFYSALLSLDTSILNQLCFIMHRYRKNLTAAKKNELVQKTKSEFNFSSKTYQEFNHYLTSMVGCLWTSKPFGKGIYIDPEILEKTGVAEYKNSLNVVHHPSFLSYAVSFLLQESPEERTVNVSSIRGKKWSWYLDYLFSQGLQGLKLFIRSSVHHSSIPRAEGINCNNQY

PTMs - Q92674 As Substrate

Q92674

Site	PTM Type	Source
T16	Phosphorylation	Uniprot
S17	Phosphorylation	Uniprot
S22	Phosphorylation	Uniprot
T25	Phosphorylation	Uniprot
K93	Ubiquitination	Uniprot
K221	Ubiquitination	Uniprot
K270	Ubiquitination	Uniprot
K277	Ubiquitination	Uniprot
S284	Phosphorylation	Uniprot
K301	Ubiquitination	Uniprot
S306	Phosphorylation	Uniprot
S308	Phosphorylation	Uniprot
S316	Phosphorylation	Uniprot
Y318	Phosphorylation	Uniprot
K325	Ubiquitination	Uniprot
T420	Phosphorylation	Uniprot
S492	Phosphorylation	Uniprot
S520	Phosphorylation	Uniprot
K619	Ubiquitination	Uniprot
K625	Ubiquitination	Uniprot
K627	Ubiquitination	Uniprot
Y637	Phosphorylation	Uniprot
H642	Phosphorylation	Uniprot
Y643	Phosphorylation	Uniprot
K659	Ubiquitination	Uniprot
K670	Ubiquitination	Uniprot
T705	Phosphorylation	Uniprot
S709	Phosphorylation	Uniprot

Research Backgrounds

Q92674_CENPI_HUMAN

Function:

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Required for the localization of CENPF, MAD1L1 and MAD2 (MAD2L1 or MAD2L2) to kinetochores. Involved in the response of gonadal tissues to follicle-stimulating hormone.

PTMs:

Sumoylated. Sumoylated form can be polyubiquitinated by RNF4, leading to its degradation. Desumoylation by SENP6 prevents its degradation.

Subcellular Location:

Nucleus. Chromosome>Centromere.
Note: Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.

Subunit Structure:

Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. Interacts with SENP6.

Family&Domains:

Belongs to the CENP-I/CTF3 family.

Restrictive clause

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CENPI Antibody - #DF2311