CD2AP Antibody - #DF2298

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Product: CD2AP Antibody
Catalog: DF2298
Description: Rabbit polyclonal antibody to CD2AP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 74 kDa; 71kD(Calculated).
Uniprot: Q9Y5K6
RRID: AB_2839524

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
CD2AP Antibody detects endogenous levels of total CD2AP.
RRID:
AB_2839524
Cite Format: Affinity Biosciences Cat# DF2298, RRID:AB_2839524.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Adapter protein CMS; AL024079; C78928; Cas ligand with multiple SH3 domains; CD2 associated protein; CD2-associated protein; CD2AP; CD2AP_HUMAN; CMS; Mesenchyme to epithelium transition protein with SH3 domains 1; METS 1; Mets1;

Immunogens

Immunogen:
Uniprot:

Q9Y5K6(CD2AP_HUMAN) >>Visit HPA database.

Gene(ID):
CD2AP(23607)
Expression:
Q9Y5K6 CD2AP_HUMAN:

Widely expressed in fetal and adult tissues.

Description:
Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis
Sequence:
MVDYIVEYDYDAVHDDELTIRVGEIIRNVKKLQEEGWLEGELNGRRGMFPDNFVKEIKRETEFKDDSLPIKRERHGNVASLVQRISTYGLPAGGIQPHPQTKNIKKKTKKRQCKVLFEYIPQNEDELELKVGDIIDINEEVEEGWWSGTLNNKLGLFPSNFVKELEVTDDGETHEAQDDSETVLAGPTSPIPSLGNVSETASGSVTQPKKIRGIGFGDIFKEGSVKLRTRTSSSETEEKKPEKPLILQSLGPKTQSVEITKTDTEGKIKAKEYCRTLFAYEGTNEDELTFKEGEIIHLISKETGEAGWWRGELNGKEGVFPDNFAVQINELDKDFPKPKKPPPPAKAPAPKPELIAAEKKYFSLKPEEKDEKSTLEQKPSKPAAPQVPPKKPTPPTKASNLLRSSGTVYPKRPEKPVPPPPPIAKINGEVSSISSKFETEPVSKLKLDSEQLPLRPKSVDFDSLTVRTSKETDVVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGGHSPTHSPEKILKLPKEEDSANLKPSELKKDTCYSPKPSVYLSTPSSASKANTTAFLTPLEIKAKVETDDVKKNSLDELRAQIIELLCIVEALKKDHGKELEKLRKDLEEEKTMRSNLEMEIEKLKKAVLSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
92
Zebrafish
92
Chicken
92
Xenopus
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5K6 As Substrate

Site PTM Type Enzyme
K55 Acetylation
K58 Sumoylation
S67 Phosphorylation
K71 Ubiquitination
S80 Phosphorylation
S86 Phosphorylation
T87 Phosphorylation
Y88 Phosphorylation
K102 Ubiquitination
S180 Phosphorylation
S189 Phosphorylation
K221 Ubiquitination
S224 Phosphorylation
K226 Ubiquitination
T229 Phosphorylation
T231 Phosphorylation
S232 Phosphorylation
S233 Phosphorylation
S234 Phosphorylation
T236 Phosphorylation
K243 Ubiquitination
S249 Phosphorylation
T254 Phosphorylation
S256 Phosphorylation
T260 Phosphorylation
T262 Phosphorylation
T264 Phosphorylation
K333 Ubiquitination
K359 Acetylation
K359 Ubiquitination
K360 Acetylation
Y361 Phosphorylation
S363 Phosphorylation
K365 Ubiquitination
K369 Ubiquitination
S380 Phosphorylation
T393 Phosphorylation
T396 Phosphorylation
K397 Methylation
K397 Ubiquitination
S405 Phosphorylation
Y409 Phosphorylation
K415 Ubiquitination
K425 Acetylation
K436 Ubiquitination
K444 Ubiquitination
K446 Ubiquitination
K457 Ubiquitination
S458 Phosphorylation
S463 Phosphorylation
T465 Phosphorylation
K470 Ubiquitination
K495 Methylation
K495 Ubiquitination
S510 Phosphorylation
T512 Phosphorylation
S514 Phosphorylation
T539 Phosphorylation
C540 S-Nitrosylation
Y541 Phosphorylation
S542 Phosphorylation
K544 Ubiquitination
S546 Phosphorylation
Y548 Phosphorylation
S550 Phosphorylation
T551 Phosphorylation
S553 Phosphorylation
S554 Phosphorylation
S556 Phosphorylation
K557 Ubiquitination
T565 Phosphorylation
K570 Ubiquitination
S582 Phosphorylation
K613 Ubiquitination
K631 Ubiquitination

Research Backgrounds

Function:

Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation (By similarity). May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell (By similarity). May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis. Plays a role in epithelial cell junctions formation.

PTMs:

Phosphorylated on tyrosine residues; probably by c-Abl, Fyn and c-Src.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell projection>Ruffle. Cell junction.
Note: Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles (PubMed:10339567). Located at podocyte slit diaphragm between podocyte foot processes (By similarity). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase (PubMed:15800069).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in fetal and adult tissues.

Subunit Structure:

Homodimer. Interacts with F-actin, PKD2, NPHS1 and NPHS2. Interacts with WTIP. Interacts with DDN; interaction is direct. Interacts (via SH3 2 domain) with CBL (via phosphorylated C-terminus). Interacts with BCAR1/p130Cas (via SH3 domain). Interacts with MVB12A and ARHGAP17. Interacts with ANLN, CD2 and CBLB. Interacts with PDCD6IP and TSG101. Interacts with RIN3. Interacts directly with RET (inactive) and CBLC; upon RET activation by GDNF suggested to dissociate from RET as CBLC:CD2AP complex (Probable) Ref.29). Interacts with CGNL1 and SH3BP1; probably part of a complex at cell junctions. Interacts with CAPZA1.

Family&Domains:

The Pro-rich domain may mediate binding to SH3 domains.

Potential homodimerization is mediated by the coiled coil domain.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

References

1). ADAM metallopeptidase domain 10 knockdown enables podocytes to resist high glucose stimulation by inhibiting pyroptosis via MAPK pathway. Experimental and Therapeutic Medicine, 2023 (PubMed: 37153901) [IF=2.7]

Application: WB    Species: Mouse    Sample:

Figure 2 ADAM10 knockdown inhibits HG-induced podocyte apoptosis. (A) Podocyte markers were evaluated using western blot analysis. (B) Podocyte apoptosis was evaluated using the TUNEL assay. (C) Apoptotic rate was obtained according to the results of the TUNEL assay. (D) Expression levels of proteins related to apoptosis were assessed using western blot analysis. ***P
2). The Yi-Qi-Bu-Shen recipe attenuates high glucose-induced podocyte injury via the inhibition of IKK-IκBα-NFκB and ERK/P38 MAPK signaling . International Journal of Clinical and Experimental Medicine, 2022 [IF=0.2]

Application: WB    Species: Human    Sample: podocytes

Figure 2. Effect of YB on HG-induced expression of nephrin, podocin and CD2AP in podocytes. A-C. Podocytes were treated with HG and YB for 24 hours and then mRNA levels of nephrin, podocin and CD2AP were determined by qRT-PCR. (*P

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