CD109 Antibody | Affinity Biosciences

WB
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Western blot analysis of CD109 expression in Mouse cerebellum tissue lysates ,The lane on the left was treated with the antigen-specific peptide.

Fig.

Product:	CD109 Antibody
Catalog:	DF2291
Description:	Rabbit polyclonal antibody to CD109
Application:	WB
Reactivity:	Human, Mouse
Mol.Wt.:	162 kDa; 162kD(Calculated).
Uniprot:	Q6YHK3
RRID:	AB_2839517

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

CD109 Antibody detects endogenous levels of total CD109.

RRID:

AB_2839517
Cite Format: Affinity Biosciences Cat# DF2291, RRID:AB_2839517.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

150 KDa TGF beta 1 binding protein; 150 KDa TGF-beta-1-binding protein; 7D1; 8A3; Activated T cell marker CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD 109; CD109; CD109 antigen; CD109_HUMAN; CPAMD7; DKFZp762L1111; E123; FLJ38569; FLJ41966; Gov system alloantigens on platelets; p180; Platelet specific Gov antigen; Platelet-specific Gov antigen; r150; RP11 525G3.1;

Immunogens

Immunogen:

Uniprot:

Q6YHK3(CD109_HUMAN) >>Visit HPA database.

Gene(ID):

CD109(135228)

Expression:

Q6YHK3 CD109_HUMAN:

Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 4 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.

Description:

Modulates negatively TGFB1 signaling in keratinocytes

Sequence:

Q6YHK3 CD109_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQGPPLLTAAHLLCVCTAALAVAPGPRFLVTAPGIIRPGGNVTIGVELLEHCPSQVTVKAELLKTASNLTVSVLEAEGVFEKGSFKTLTLPSLPLNSADEIYELRVTGRTQDEILFSNSTRLSFETKRISVFIQTDKALYKPKQEVKFRIVTLFSDFKPYKTSLNILIKDPKSNLIQQWLSQQSDLGVISKTFQLSSHPILGDWSIQVQVNDQTYYQSFQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMVVSRGQLVAVGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIKLYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLGMFMNSFAVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVELQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPTASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSINGLASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNKSPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYTLALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMNTERTNIQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPTAVNISANGFGFAICQLNVVYNVKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNLLSGFMVPSEAISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFKLLYFMELWL

PTMs - Q6YHK3 As Substrate

Q6YHK3

Site	PTM Type	Source
T31	Phosphorylation	Uniprot
N68	N-Glycosylation	Uniprot
Y102	Phosphorylation	Uniprot
N118	N-Glycosylation	Uniprot
K143	Ubiquitination	Uniprot
K169	Ubiquitination	Uniprot
N247	N-Glycosylation	Uniprot
K417	Ubiquitination	Uniprot
N419	N-Glycosylation	Uniprot
Y446	Phosphorylation	Uniprot
S450	Phosphorylation	Uniprot
K465	Ubiquitination	Uniprot
K478	Ubiquitination	Uniprot
K491	Acetylation	Uniprot
K491	Ubiquitination	Uniprot
T581	Phosphorylation	Uniprot
S585	Phosphorylation	Uniprot
Y660	Phosphorylation	Uniprot
T833	Phosphorylation	Uniprot
T840	Phosphorylation	Uniprot
K858	Ubiquitination	Uniprot
T946	Phosphorylation	Uniprot
K950	Ubiquitination	Uniprot
K1022	Ubiquitination	Uniprot
S1036	Phosphorylation	Uniprot
S1214	Phosphorylation	Uniprot
S1216	Phosphorylation	Uniprot
Y1384	Phosphorylation	Uniprot

Research Backgrounds

Q6YHK3_CD109_HUMAN

Function:

Modulates negatively TGFB1 signaling in keratinocytes.

PTMs:

N-glycosylated.

2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form.

Subcellular Location:

Cell membrane>Lipid-anchor.

Tissue Specificity:

Subunit Structure:

Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.

Family&Domains:

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.

References

1). Aptamer-based CRISPR/Cas12a assay for the ultrasensitive detection of extracellular vesicle proteins. TALANTA, 2021 (PubMed: 33076176) [IF=6.1]

Application: WB Species: human Sample: SUNE2 cells

Fig. 5. Evaluation of the specificity of the aptamer-CRISPR/Cas12a assay. a Schematics showing the sequence variation at different positions in CD109-apt. b CRISPR/Cas12a detection of single-strand and double-strand CD109-apt with mismatches at different positions (1–2, 3–4, 5–6, 1–4, 3–6). c Western blot analysis of the level of CD109 in SUNE2 cells after transfection with siNC and siCD109 1#, 2#, and 3#. d Histogram analysis of CD109+ EVs in the supernatant of SUNE2 cells treated with siCD109 1#, 2#, and 3# and siNC. e Histogram analysis of CD109+ EVs in the supernatants from NPC cells (HK1, HONE1, and SUNE2 cells) and normal nasopharyngeal cells (NEPC1 and NEPC2 cells). Data are based on 3 replicates; error bars = standard errors. f Histogram analysis of CD109+ EV and EGFR+ EV levels in sera from NPC patients and healthy controls. Data are based on 3 replicates; error bars = standard errors. NEPC2, NEPC2 Bmi-1.

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CD109 Antibody - #DF2291