Product: CD109 Antibody
Catalog: DF2291
Description: Rabbit polyclonal antibody to CD109
Application: WB
Reactivity: Human, Mouse
Mol.Wt.: 162 kDa; 162kD(Calculated).
Uniprot: Q6YHK3
RRID: AB_2839517

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
CD109 Antibody detects endogenous levels of total CD109.
RRID:
AB_2839517
Cite Format: Affinity Biosciences Cat# DF2291, RRID:AB_2839517.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

150 KDa TGF beta 1 binding protein; 150 KDa TGF-beta-1-binding protein; 7D1; 8A3; Activated T cell marker CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD 109; CD109; CD109 antigen; CD109_HUMAN; CPAMD7; DKFZp762L1111; E123; FLJ38569; FLJ41966; Gov system alloantigens on platelets; p180; Platelet specific Gov antigen; Platelet-specific Gov antigen; r150; RP11 525G3.1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q6YHK3 CD109_HUMAN:

Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 4 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.

Description:
Modulates negatively TGFB1 signaling in keratinocytes
Sequence:
MQGPPLLTAAHLLCVCTAALAVAPGPRFLVTAPGIIRPGGNVTIGVELLEHCPSQVTVKAELLKTASNLTVSVLEAEGVFEKGSFKTLTLPSLPLNSADEIYELRVTGRTQDEILFSNSTRLSFETKRISVFIQTDKALYKPKQEVKFRIVTLFSDFKPYKTSLNILIKDPKSNLIQQWLSQQSDLGVISKTFQLSSHPILGDWSIQVQVNDQTYYQSFQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMVVSRGQLVAVGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIKLYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLGMFMNSFAVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVELQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPTASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSINGLASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNKSPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYTLALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMNTERTNIQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPTAVNISANGFGFAICQLNVVYNVKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNLLSGFMVPSEAISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFKLLYFMELWL

PTMs - Q6YHK3 As Substrate

Site PTM Type Enzyme
T31 Phosphorylation
N68 N-Glycosylation
Y102 Phosphorylation
N118 N-Glycosylation
K143 Ubiquitination
K169 Ubiquitination
N247 N-Glycosylation
K417 Ubiquitination
N419 N-Glycosylation
Y446 Phosphorylation
S450 Phosphorylation
K465 Ubiquitination
K478 Ubiquitination
K491 Acetylation
K491 Ubiquitination
T581 Phosphorylation
S585 Phosphorylation
Y660 Phosphorylation
T833 Phosphorylation
T840 Phosphorylation
K858 Ubiquitination
T946 Phosphorylation
K950 Ubiquitination
K1022 Ubiquitination
S1036 Phosphorylation
S1214 Phosphorylation
S1216 Phosphorylation
Y1384 Phosphorylation

Research Backgrounds

Function:

Modulates negatively TGFB1 signaling in keratinocytes.

PTMs:

N-glycosylated.

2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form.

Subcellular Location:

Cell membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 4 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.

Subunit Structure:

Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.

Family&Domains:

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.

References

1). Aptamer-based CRISPR/Cas12a assay for the ultrasensitive detection of extracellular vesicle proteins. TALANTA, 2021 (PubMed: 33076176) [IF=6.1]

Application: WB    Species: human    Sample: SUNE2 cells

Fig. 5. Evaluation of the specificity of the aptamer-CRISPR/Cas12a assay. a Schematics showing the sequence variation at different positions in CD109-apt. b CRISPR/Cas12a detection of single-strand and double-strand CD109-apt with mismatches at different positions (1–2, 3–4, 5–6, 1–4, 3–6). c Western blot analysis of the level of CD109 in SUNE2 cells after transfection with siNC and siCD109 1#, 2#, and 3#. d Histogram analysis of CD109+ EVs in the supernatant of SUNE2 cells treated with siCD109 1#, 2#, and 3# and siNC. e Histogram analysis of CD109+ EVs in the supernatants from NPC cells (HK1, HONE1, and SUNE2 cells) and normal nasopharyngeal cells (NEPC1 and NEPC2 cells). Data are based on 3 replicates; error bars = standard errors. f Histogram analysis of CD109+ EV and EGFR+ EV levels in sera from NPC patients and healthy controls. Data are based on 3 replicates; error bars = standard errors. NEPC2, NEPC2 Bmi-1.

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