CCNT2 Antibody - #DF2289

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Product: CCNT2 Antibody
Catalog: DF2289
Description: Rabbit polyclonal antibody to CCNT2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 81kDa; 81kD(Calculated).
Uniprot: O60583
RRID: AB_2839515

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
CCNT2 Antibody detects endogenous levels of total CCNT2.
RRID:
AB_2839515
Cite Format: Affinity Biosciences Cat# DF2289, RRID:AB_2839515.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CCNT2; CCNT2_HUMAN; Cyclin T2a; Cyclin T2b; Cyclin-T2; CycT2; FLJ90560; MGC134840; SDS stable vimentin bound DNA fragment HEF42VIM22; Subunit of positive elongation transcription factor b;

Immunogens

Immunogen:
Uniprot:

O60583(CCNT2_HUMAN) >>Visit HPA database.

Gene(ID):
CCNT2(905)
Expression:
O60583 CCNT2_HUMAN:

Ubiquitously expressed.

Description:
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II).
Sequence:
MASGRGASSRWFFTREQLENTPSRRCGVEADKELSCRQQAANLIQEMGQRLNVSQLTINTAIVYMHRFYMHHSFTKFNKNIISSTALFLAAKVEEQARKLEHVIKVAHACLHPLEPLLDTKCDAYLQQTQELVILETIMLQTLGFEITIEHPHTDVVKCTQLVRASKDLAQTSYFMATNSLHLTTFCLQYKPTVIACVCIHLACKWSNWEIPVSTDGKHWWEYVDPTVTLELLDELTHEFLQILEKTPNRLKKIRNWRANQAARKPKVDGQVSETPLLGSSLVQNSILVDSVTGVPTNPSFQKPSTSAFPAPVPLNSGNISVQDSHTSDNLSMLATGMPSTSYGLSSHQEWPQHQDSARTEQLYSQKQETSLSGSQYNINFQQGPSISLHSGLHHRPDKISDHSSVKQEYTHKAGSSKHHGPISTTPGIIPQKMSLDKYREKRKLETLDLDVRDHYIAAQVEQQHKQGQSQAASSSSVTSPIKMKIPIANTEKYMADKKEKSGSLKLRIPIPPTDKSASKEELKMKIKVSSSERHSSSDEGSGKSKHSSPHISRDHKEKHKEHPSSRHHTSSHKHSHSHSGSSSGGSKHSADGIPPTVLRSPVGLSSDGISSSSSSSRKRLHVNDASHNHHSKMSKSSKSSGSSSSSSSSVKQYISSHNSVFNHPLPPPPPVTYQVGYGHLSTLVKLDKKPVETNGPDANHEYSTSSQHMDYKDTFDMLDSLLSAQGMNM

PTMs - O60583 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation
S9 Phosphorylation
T14 Phosphorylation
K32 Ubiquitination
K99 Ubiquitination
K407 Sumoylation
T426 Phosphorylation
S475 Phosphorylation
S477 Phosphorylation
T479 Phosphorylation
S480 Phosphorylation
K485 Ubiquitination
K493 Acetylation
S502 Phosphorylation
T514 Phosphorylation
K520 Acetylation
S536 Phosphorylation
S538 Phosphorylation
S548 Phosphorylation
S549 Phosphorylation
S590 Phosphorylation
T597 Phosphorylation
S601 Phosphorylation
S617 Phosphorylation
S645 Phosphorylation

Research Backgrounds

Function:

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II). The activity of this complex is regulated by binding with 7SK snRNA. Plays a role during muscle differentiation; P-TEFB complex interacts with MYOD1; this tripartite complex promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation and binds the chromatin of promoters and enhancers of muscle-specific genes; this event correlates with hyperphosphorylation of the CTD domain of RNA pol II (By similarity). In addition, enhances MYOD1-dependent transcription through interaction with PKN1. Involved in early embryo development (By similarity).

(Microbial infection) Promotes transcriptional activation of early and late herpes simplex virus 1/HHV-1 promoters.

Subcellular Location:

Cytoplasm>Perinuclear region. Nucleus.
Note: Nucleus in differentiating cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with CDK9 to form P-TEFb. Interacts with POLR2A (via the C-terminal domain (CTD)); mediates transcriptional activity. Interacts with HEXIM1; mediates formation of a tripartite complex with KPNA2. Interacts with HEXIM2. Interacts with PKN1; enhances MYOD1-dependent transcription. P-TEFB complex interacts with RB1; promotes phosphorylation of RB1. P-TEFB complex interacts with MYOD1; promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation (By similarity). Interacts with MDFI and MDFIC. Interacts with MON1B; downregulates CCNT2-mediated activation of viral promoters during herpes simplex virus 1/HHV-1 infection.

(Microbial infection) Interacts with HIV-2 and SIV Tat. Does not bind efficiently to the transactivation domain of the HIV-1 Tat.

Family&Domains:

Belongs to the cyclin family. Cyclin C subfamily.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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