CCNG2 Antibody - #DF2284

Fig. 8 | LncRNA AK023391 was involved in the regulation of the PI3K/Akt signaling pathway.e-f Western blotting validation of the effects of AK023391 knockdown on the expression of PI3K/Akt, NF-κB, p53, and FOXO3a pathways, and their downstream transcription factors c-myb, cyclinB1/G2, and BCL-6 in HGC 27, AGS, and SGC-7901 cells

Fig. 1| Cyclin G2 expression is down-regulated in gastric cancer..e Comparison of the protein expression levels of cyclin G2 in three gastric cell lines and human normal gastric epithelial cell line

Fig. 2| Cyclin G2 inhibits the proliferation and migration of SGC-7901 or MGC-803 gastric cancer cells.. i and j Representative Transwell® migration assays in cyclin G2 overexpression SGC-7901 or MGC-803 cells determined by stained with Giemsa. The migration ratio is expressed as a percentage of the control cells at the indicated time-points (n = 3). **P < 0.01

Fig. 5| Cyclin G2 inhibits Wnt/β-catenin signaling through DPR1. a Cell lysates from COS-7 cells were immunoprecipitated with Dpr1 or IgG control antibody, followed by western blot analysis with a cyclin G2 antibody (upper panel). Total cellular protein from COS-7 cells was immunoprecipitated with an anti-cyclin G2 or IgG control antibody, then blotted with anti-DPR1 and anti-Dvl2 antibody, respectively (middle panel). Western blot analysis of endogenous Dpr1 and cyclin G2 protein level in COS-7 cells (lower panel). b Direct interaction of Dpr1 and cyclin G2 was detected using Duolink in situ PLA. Red spots represent the interaction of Dpr1 and cyclin G2. The nuclei were stained using DAPI and are shown in blue. Images were acquired using a confocal microscopy with a 40× objective.

Fig. 1 Knockout of cyclin G2 in macrophages attenuates the tumor suppressive effects of IFN-γ. A CCNG2 mRNA expression in THP-1 cells treated with IFN-γ, LPS, IL-4 and IL-13 was detected RT–qPCR using β-actin as an internal control. Data are presented as the mean ± SD (Data of CCNG2 mRNA expression in THP-1 cells treated with IFN-γ representing 3 independent experiments). B Cyclin G2 protein levels in THP-1 cells treated with IFN-γ, IL-4 and IL-13 were evaluated by western blotting using β-tubulin as a loading control (representing 3 independent experiments). C Cyclin G2 protein levels in the human peripheral blood monocytes were determined by western blotting following IFN-γ, IL-4 and IL-13 treatment. β-tubulin was used as a loading control (representing 3 independent experiments). D After IFN-γ (100 ng/mL) treatment of BMDMs, the cyclin G2 protein expression was detected by western blotting. β-tubulin was used as a loading control (representing 3 independent experiments). E BMDMs were isolated from WT and Ccng2−/− C57BL/6 mice and identified by western blotting. β-tubulin was used as a loading control. F LLC cells were mixed with BMDMs from WT and Ccng2−/− C57BL/6 mice at a ratio of 5:1 and injected subcutaneously into C57BL/6 mice, which were then treated with IFN-γ at specific times. G–J Gross tumors (G), tumor growth curves (H), and tumor weights (I) and volumes (J) measured at the study endpoint. (n = 5). K Representative Ki-67 immunohistochemical staining of tumors from mice in the WT and Ccng2−/− groups. 10× scale bar = 200 μm; 40× scale bar = 50 μm. (L) Graph showing the number of Ki-67-positive cells in each field (n = 5). The data in H, I, J, and L are presented as the mean ± SEM. Data presented in A, I, J, and L were analyzed with the unpaired Student’s t-test. Data in H were analyzed using two-way ANOVA. *p