Product: UBE2R2 Antibody
Catalog: DF2262
Description: Rabbit polyclonal antibody to UBE2R2
Application: WB IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 27 kDa,17kDa; 27kD(Calculated).
Uniprot: Q712K3
RRID: AB_2839491

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
UBE2R2 Antibody detects endogenous levels of total UBE2R2.
RRID:
AB_2839491
Cite Format: Affinity Biosciences Cat# DF2262, RRID:AB_2839491.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDC34B; E2 CDC34B; E2 ubiquitin conjugating enzyme R2; FLJ20419; MGC10481; UB2R2; UB2R2_HUMAN; UBC3B; UBE2R2; Ubiquitin carrier protein R2; Ubiquitin conjugating enzyme E2 CDC34B; Ubiquitin conjugating enzyme E2 R2; Ubiquitin conjugating enzyme E2R 2; Ubiquitin conjugating enzyme UBC3B; Ubiquitin protein ligase R2; Ubiquitin-conjugating enzyme E2 R2; Ubiquitin-conjugating enzyme E2-CDC34B; Ubiquitin-protein ligase R2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination. May be involved in degradation of katenin
Sequence:
MAQQQMTSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKEYAEIIRKQVSATKAEAEKDGVKVPTTLAEYCIKTKVPSNDNSSDLLYDDLYDDDIDDEDEEEEDADCYDDDDSGNEES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q712K3 As Substrate

Site PTM Type Enzyme
K11 Ubiquitination
K18 Ubiquitination
K63 Ubiquitination
K157 Ubiquitination
K159 Ubiquitination
R166 Methylation
K167 Ubiquitination
K173 Ubiquitination
K182 Ubiquitination
Y190 Phosphorylation
K193 Ubiquitination
K195 Ubiquitination
S202 Phosphorylation
Y207 Phosphorylation
S233 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1)
S238 Phosphorylation

Research Backgrounds

Function:

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination. May be involved in degradation of katenin.

Subunit Structure:

When phosphorylated, interacts with beta-TrCP (BTRC).

Family&Domains:

Belongs to the ubiquitin-conjugating enzyme family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

Restrictive clause

 

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