USP48 Antibody - #DF2260

(1)  
Product: USP48 Antibody
Catalog: DF2260
Description: Rabbit polyclonal antibody to USP48
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 145 kDa; 119kD(Calculated).
Uniprot: Q86UV5
RRID: AB_2839489

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(92%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
USP48 Antibody detects endogenous levels of total USP48.
RRID:
AB_2839489
Cite Format: Affinity Biosciences Cat# DF2260, RRID:AB_2839489.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AAH67261; Deubiquitinating enzyme 48; DKFZp762M1713; EC 3.1.2.15; Kiaa4202; MGC132556; MGC14879; RAP1GA1; Synaptic ubiquitin specific protease; synUSP; Ubiquitin carboxyl terminal hydrolase 48; Ubiquitin carboxyl-terminal hydrolase 48; Ubiquitin specific peptidase 48; Ubiquitin specific processing protease 48; Ubiquitin specific protease 31; Ubiquitin specific protease 48; Ubiquitin thioesterase 48; Ubiquitin thiolesterase 48; Ubiquitin-specific-processing protease 48; UBP48_HUMAN; USP31; USP48;

Immunogens

Immunogen:
Uniprot:

Q86UV5(UBP48_HUMAN) >>Visit HPA database.

Gene(ID):
USP48(84196)
Expression:
Q86UV5 UBP48_HUMAN:

Widely expressed.

Description:
Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at post-synaptic sites.
Sequence:
MAPRLQLEKAAWRWAETVRPEEVSQEHIETAYRIWLEPCIRGVCRRNCKGNPNCLVGIGEHIWLGEIDENSFHNIDDPNCERRKKNSFVGLTNLGATCYVNTFLQVWFLNLELRQALYLCPSTCSDYMLGDGIQEEKDYEPQTICEHLQYLFALLQNSNRRYIDPSGFVKALGLDTGQQQDAQEFSKLFMSLLEDTLSKQKNPDVRNIVQQQFCGEYAYVTVCNQCGRESKLLSKFYELELNIQGHKQLTDCISEFLKEEKLEGDNRYFCENCQSKQNATRKIRLLSLPCTLNLQLMRFVFDRQTGHKKKLNTYIGFSEILDMEPYVEHKGGSYVYELSAVLIHRGVSAYSGHYIAHVKDPQSGEWYKFNDEDIEKMEGKKLQLGIEEDLAEPSKSQTRKPKCGKGTHCSRNAYMLVYRLQTQEKPNTTVQVPAFLQELVDRDNSKFEEWCIEMAEMRKQSVDKGKAKHEEVKELYQRLPAGAEPYEFVSLEWLQKWLDESTPTKPIDNHACLCSHDKLHPDKISIMKRISEYAADIFYSRYGGGPRLTVKALCKECVVERCRILRLKNQLNEDYKTVNNLLKAAVKGSDGFWVGKSSLRSWRQLALEQLDEQDGDAEQSNGKMNGSTLNKDESKEERKEEEELNFNEDILCPHGELCISENERRLVSKEAWSKLQQYFPKAPEFPSYKECCSQCKILEREGEENEALHKMIANEQKTSLPNLFQDKNRPCLSNWPEDTDVLYIVSQFFVEEWRKFVRKPTRCSPVSSVGNSALLCPHGGLMFTFASMTKEDSKLIALIWPSEWQMIQKLFVVDHVIKITRIEVGDVNPSETQYISEPKLCPECREGLLCQQQRDLREYTQATIYVHKVVDNKKVMKDSAPELNVSSSETEEDKEEAKPDGEKDPDFNQSNGGTKRQKISHQNYIAYQKQVIRRSMRHRKVRGEKALLVSANQTLKELKIQIMHAFSVAPFDQNLSIDGKILSDDCATLGTLGVIPESVILLKADEPIADYAAMDDVMQVCMPEEGFKGTGLLGH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
92
Rabbit
92
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86UV5 As Substrate

Site PTM Type Enzyme
K9 Ubiquitination
Y162 Phosphorylation
K170 Ubiquitination
K187 Ubiquitination
K199 Ubiquitination
K231 Ubiquitination
K258 Ubiquitination
R267 Methylation
K276 Ubiquitination
Y326 Phosphorylation
K368 Ubiquitination
K376 Ubiquitination
K381 Ubiquitination
S394 Phosphorylation
K395 Ubiquitination
T398 Phosphorylation
K425 Ubiquitination
K468 Ubiquitination
K473 Ubiquitination
K505 Ubiquitination
K518 Ubiquitination
K523 Ubiquitination
K551 Ubiquitination
K555 Ubiquitination
K568 Ubiquitination
K576 Ubiquitination
K583 Ubiquitination
K587 Ubiquitination
K596 Ubiquitination
S620 Phosphorylation
S627 Phosphorylation
T628 Phosphorylation
K669 Ubiquitination
K674 Ubiquitination
K681 Sumoylation
K681 Ubiquitination
K689 Ubiquitination
K696 Ubiquitination
K710 Ubiquitination
K717 Ubiquitination
T820 Phosphorylation
K839 Ubiquitination
Y859 Phosphorylation
Y865 Phosphorylation
K868 Ubiquitination
S886 Phosphorylation
S887 Phosphorylation
S888 Phosphorylation
S910 Phosphorylation
K918 Ubiquitination
Y924 Phosphorylation
K929 Ubiquitination
S935 Phosphorylation
K945 Ubiquitination
K956 Acetylation
K956 Ubiquitination
K1028 Ubiquitination

Research Backgrounds

Function:

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts with TRAF2 and RELA.

Family&Domains:

Belongs to the peptidase C19 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.