Product: EIF2B2 Antibody
Catalog: DF2254
Description: Rabbit polyclonal antibody to EIF2B2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 39 kDa.; 39kD(Calculated).
Uniprot: P49770
RRID: AB_2839483

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
EIF2B2 Antibody detects endogenous levels of total EIF2B2.
RRID:
AB_2839483
Cite Format: Affinity Biosciences Cat# DF2254, RRID:AB_2839483.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EI2BB_HUMAN; EIF 2Bbeta; EIF-2Bbeta; EIF2B; EIF2B GDP GTP exchange factor subunit beta; EIF2B2; EIF2B2 antibody; EIF2BB; Eukaryotic translation initiation factor 2B; Eukaryotic translation initiation factor 2B beta; Eukaryotic translation initiation factor 2B, subunit 2; Eukaryotic translation initiation factor 2B, subunit 2 beta; Eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa; S20I15; S20III15; Translation initiation factor eIF-2B subunit beta;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
92
Chicken
83
Xenopus
75
Zebrafish
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49770 As Substrate

Site PTM Type Enzyme
K7 Ubiquitination
K23 Ubiquitination
T47 Phosphorylation
K87 Ubiquitination
S100 Phosphorylation
S103 Phosphorylation
T173 Phosphorylation
K179 Ubiquitination
K231 Ubiquitination
T236 Phosphorylation
K237 Ubiquitination
T238 Phosphorylation
K273 Ubiquitination
K287 Ubiquitination

Research Backgrounds

Function:

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

Subunit Structure:

Complex of five different subunits; alpha, beta, gamma, delta and epsilon.

Family&Domains:

Belongs to the eIF-2B alpha/beta/delta subunits family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.