SLC12A2 Antibody - #DF2245

(3)   (7)  
Product: SLC12A2 Antibody
Catalog: DF2245
Description: Rabbit polyclonal antibody to SLC12A2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 130 kDa; 131kD(Calculated).
Uniprot: P55011
RRID: AB_2839476

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(85%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
SLC12A2 Antibody detects endogenous levels of total SLC12A2.
RRID:
AB_2839476
Cite Format: Affinity Biosciences Cat# DF2245, RRID:AB_2839476.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Basolateral Na-K-Cl symporter; BSC; BSC2; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; mBSC2; MGC104233; NKCC1; PPP1R141; Protein phosphatase 1, regulatory subunit 141; S12A2_HUMAN; Slc12a2; sodium-potassium-chloride cotransporter 1; Solute carrier family 12 (sodium/potassium/chloride transporter), member 2; solute carrier family 12 (sodium/potassium/chloride transporters) member 2; Solute carrier family 12 member 2; sy-ns;

Immunogens

Immunogen:
Uniprot:

P55011(S12A2_HUMAN) >>Visit HPA database.

Gene(ID):
SLC12A2(6558)
Expression:
P55011 S12A2_HUMAN:

Expressed in many tissues.

Description:
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume
Sequence:
MEPRPTAPSSGAPGLAGVGETPSAAALAAARVELPGTAVPSVPEDAAPASRDGGGVRDEGPAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAAAAAAAAAAAGAGAGAKQTPADGEASGESEPAKGSEEAKGRFRVNFVDPAASSSAEDSLSDAAGVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Zebrafish
92
Chicken
85
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55011 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S23 Phosphorylation
T75 Phosphorylation
S77 Phosphorylation P54646 (PRKAA2) , Q13131 (PRKAA1)
S79 Phosphorylation
K114 Ubiquitination
S123 Phosphorylation
K130 Ubiquitination
S132 Phosphorylation
K136 Ubiquitination
T203 Phosphorylation Q9UEW8 (STK39) , O95747 (OXSR1)
T207 Phosphorylation Q9UEW8 (STK39) , O95747 (OXSR1)
Y208 Phosphorylation
T212 Phosphorylation Q9BYP7 (WNK3) , O95747 (OXSR1) , Q9UEW8 (STK39)
T217 Phosphorylation Q9BYP7 (WNK3) , O95747 (OXSR1)
Y227 Phosphorylation
T230 Phosphorylation O95747 (OXSR1)
K237 Ubiquitination
S242 Phosphorylation P54646 (PRKAA2)
K252 Ubiquitination
S265 Phosphorylation
T266 Phosphorylation
T268 Phosphorylation
Y275 Phosphorylation
T276 Phosphorylation
K280 Ubiquitination
Y353 Phosphorylation
K833 Methylation
K833 Ubiquitination
S836 Phosphorylation
K841 Ubiquitination
Y842 Phosphorylation
Y855 Phosphorylation
K923 Ubiquitination
S940 Phosphorylation
K943 Ubiquitination
S944 Phosphorylation
T947 Phosphorylation
K948 Ubiquitination
S953 Phosphorylation
Y956 Phosphorylation
S957 Phosphorylation
K958 Ubiquitination
K959 Ubiquitination
K966 Ubiquitination
K971 Ubiquitination
K976 Ubiquitination
K983 Ubiquitination
T984 Phosphorylation
K991 Ubiquitination
S994 Phosphorylation
K995 Ubiquitination
K1007 Ubiquitination
S1012 Phosphorylation
K1017 Ubiquitination
K1061 Ubiquitination
T1074 Phosphorylation
K1078 Ubiquitination
K1095 Ubiquitination
K1097 Ubiquitination
K1098 Sumoylation
K1098 Ubiquitination
K1118 Ubiquitination
K1125 Ubiquitination
K1127 Ubiquitination
K1143 Ubiquitination
Y1211 Phosphorylation
S1212 Phosphorylation

Research Backgrounds

Function:

Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in many tissues.

Family&Domains:

Belongs to the SLC12A transporter family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Activated WNK3 induced by intracerebral hemorrhage deteriorates brain injury maybe via WNK3/SPAK/NKCC1 pathway. EXPERIMENTAL NEUROLOGY, 2020 (PubMed: 32589890) [IF=5.3]

Application: WB    Species: Rat    Sample: brain tissue

Fig. 6. Effects of WNK3 on the levels of p-SPAK and p-NKCC1 in rat brain tissue after ICH. (A) Levels of WNK3 protein were significantly changed in various treated ICH groups. (B) The levels of p-SPAK protein were significantly changed in various groups. (C) The levels of p-NKCC1 protein were significantly changed in various groups. Data are presented as the mean ± SD. **P < .01 vs. Sham group; ns, no significant differences vs. ICH group; ##P < .01 vs. ICH + si-NC group; && P < .01 vs. ICH + Vector group; n = 6.
2). Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model. Nutrition & Metabolism, 2021 (PubMed: 33691721) [IF=4.5]

Application: WB    Species: Human    Sample: NAFLD

Fig. 4 Identification of the changed expression levels of proteins in NAFLD with and without Kaili Sour Soup treatment. a and b Western blot analysis of the protein expression in NAFLD with and without Kaili Sour Soup treatment. β-Actin served as a reference control
3). Upregulation of ITGB6 in primary palmar hyperhidrosis. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 2023 (PubMed: 37212774) [IF=2.1]

Application: WB    Species: Human    Sample:

Fig. 3. Aquaporin 5 (AQP5) and Na-K-Cl cotransporter 1 (NKCC1) protein expression levels in sweat gland cells extracted from patients with primary palmar hyperhidrosis treated with blank control or transfected with overexpression negative control (OE NC) or integrin β6 (ITGB6) OE. Western blot images (A) and quantitative analyses (B) (ITGB6 OE compared to control, AQP5: Tukey’s honest significant difference (HSD), p = 0.055; NKCC1: Tukey’s HSD, p = 0.190). The data are presented as mean ± 95% confidence interval (95% CI)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.