Product: SLC5A5 Antibody
Catalog: DF2242
Description: Rabbit polyclonal antibody to SLC5A5
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 69 kDa; 69kD(Calculated).
Uniprot: Q92911
RRID: AB_2839473

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SLC5A5 Antibody detects endogenous levels of total SLC5A5.
RRID:
AB_2839473
Cite Format: Affinity Biosciences Cat# DF2242, RRID:AB_2839473.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na+/I- cotransporter; Na+/I-symporter; NIS; SC5A5_HUMAN; Slc5a5; sodium iodide symporter; Sodium-iodide symporter; Sodium/iodide cotransporter; Solute carrier family 5 (sodium iodide symporter) member 5; solute carrier family 5 (sodium/iodide cotransporter), member 5; Solute carrier family 5 member 5; TDH1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q92911 SC5A5_HUMAN:

Expression is primarily in thyroid tissue, but also to a lower extent in mammary gland and ovary. Expression is reduced in tumors.

Description:
Mediates iodide uptake in the thyroid gland.
Sequence:
MEAVETGERPTFGAWDYGVFALMLLVSTGIGLWVGLARGGQRSAEDFFTGGRRLAALPVGLSLSASFMSAVQVLGVPSEAYRYGLKFLWMCLGQLLNSVLTALLFMPVFYRLGLTSTYEYLEMRFSRAVRLCGTLQYIVATMLYTGIVIYAPALILNQVTGLDIWASLLSTGIICTFYTAVGGMKAVVWTDVFQVVVMLSGFWVVLARGVMLVGGPRQVLTLAQNHSRINLMDFNPDPRSRYTFWTFVVGGTLVWLSMYGVNQAQVQRYVACRTEKQAKLALLINQVGLFLIVSSAACCGIVMFVFYTDCDPLLLGRISAPDQYMPLLVLDIFEDLPGVPGLFLACAYSGTLSTASTSINAMAAVTVEDLIKPRLRSLAPRKLVIISKGLSLIYGSACLTVAALSSLLGGGVLQGSFTVMGVISGPLLGAFILGMFLPACNTPGVLAGLGAGLALSLWVALGATLYPPSEQTMRVLPSSAARCVALSVNASGLLDPALLPANDSSRAPSSGMDASRPALADSFYAISYLYYGALGTLTTVLCGALISCLTGPTKRSTLAPGLLWWDLARQTASVAPKEEVAILDDNLVKGPEELPTGNKKPPGFLPTNEDRLFFLGQKELEGAGSWTPCVGHDGGRDQQETNL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92911 As Substrate

Site PTM Type Enzyme
S43 Phosphorylation
S66 Phosphorylation
S126 Phosphorylation
S227 Phosphorylation
S377 Phosphorylation

Research Backgrounds

Function:

Mediates iodide uptake in the thyroid gland.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expression is primarily in thyroid tissue, but also to a lower extent in mammary gland and ovary. Expression is reduced in tumors.

Family&Domains:

Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Research Fields

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

References

1). Long Non-coding Antisense RNA TNRC6C-AS1 Is Activated in Papillary Thyroid Cancer and Promotes Cancer Progression by Suppressing TNRC6C Expression. Frontiers in Endocrinology, 2018 (PubMed: 30038597) [IF=5.2]

Application: WB    Species: human    Sample: TPC1 cells

FIGURE 6 | Downregulation of TNRC6C-AS1 or overexpression of TNRC6C promotes iodine uptake of TPC1 cells. (A) Downregulation of TNRC6C-AS1 by transfecting of siRNA-TNRC6C-AS1 for 48 h caused significantly increased mRNA expression of NIS, TPO, TSHR and Pendrin in TPC1 cells compared to control cells. (B) Downregulation of TNRC6C-AS1 by transfecting of siRNA-TNRC6C-AS1 for 48 h showed significant increase of protein levels of NIS, TPO, TSHR and Pendrin in TPC1 cells (GAPDH was used as the internal control).

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