RIOK2 Antibody - #DF2234

(3)  
Product: RIOK2 Antibody
Catalog: DF2234
Description: Rabbit polyclonal antibody to RIOK2
Application: WB IHC IF/ICC
Reactivity: Human
Mol.Wt.: 63 kDa; 63kD(Calculated).
Uniprot: Q9BVS4
RRID: AB_2839465

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
RIOK2 Antibody detects endogenous levels of total RIOK2.
RRID:
AB_2839465
Cite Format: Affinity Biosciences Cat# DF2234, RRID:AB_2839465.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ11159; OTTHUMP00000158911; OTTHUMP00000222868; RIO kinase 2 (yeast); RIO kinase 2; RIO2; RIOK2; RIOK2_HUMAN; Serine/threonine protein kinase RIO2; Serine/threonine-protein kinase rio2;

Immunogens

Immunogen:
Uniprot:

Q9BVS4(RIOK2_HUMAN) >>Visit HPA database.

Gene(ID):
RIOK2(55781)
Sequence:
MGKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE

PTMs - Q9BVS4 As Substrate

Site PTM Type Enzyme
K8 Ubiquitination
T21 Phosphorylation
K45 Ubiquitination
K51 Ubiquitination
K86 Ubiquitination
T130 Phosphorylation
S131 Phosphorylation
K160 Ubiquitination
Y181 Phosphorylation
K270 Ubiquitination
K275 Ubiquitination
K288 Ubiquitination
S332 Phosphorylation
S335 Phosphorylation P53350 (PLK1)
S337 Phosphorylation
Y348 Phosphorylation
S350 Phosphorylation
S354 Phosphorylation
S362 Phosphorylation
Y366 Phosphorylation
S369 Phosphorylation
S370 Phosphorylation
K377 Ubiquitination
S380 Phosphorylation P53350 (PLK1)
S382 Phosphorylation
S385 Phosphorylation
S390 Phosphorylation
S412 Phosphorylation
T414 Phosphorylation
S417 Phosphorylation
K420 Ubiquitination
Y426 Phosphorylation
S442 Phosphorylation
Y445 Phosphorylation
S457 Phosphorylation
T481 Phosphorylation
S483 Phosphorylation
T485 Phosphorylation
S486 Phosphorylation
S487 Phosphorylation
S489 Phosphorylation
T510 Phosphorylation
K523 Ubiquitination
K531 Methylation
K531 Ubiquitination
S548 Phosphorylation P53350 (PLK1)

Research Backgrounds

Function:

Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA. Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function.

PTMs:

Autophosphorylated (in vitro). Phosphorylation at Ser-335, Ser-380, Ser-548 by PLK1 affects the timing of the metaphase-anaphase transition.

Subcellular Location:

Cytoplasm.
Note: Exported out of the nucleus via its NES in a XPO1-dependent manner.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Associated with late 40S pre-ribosomal particles. Interacts with PLK1 (via its N-terminus).

Family&Domains:

Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

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