ATP12A Antibody - #DF2223

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Product: ATP12A Antibody
Catalog: DF2223
Description: Rabbit polyclonal antibody to ATP12A
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 115kDa; 116kD(Calculated).
Uniprot: P54707
RRID: AB_2839454

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(83%), Horse(83%), Sheep(92%), Rabbit(92%), Dog(83%), Chicken(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
ATP12A Antibody detects endogenous levels of total ATP12A.
RRID:
AB_2839454
Cite Format: Affinity Biosciences Cat# DF2223, RRID:AB_2839454.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP1AL1; ATPase H+/K+ transporting nongastric alpha polypeptide; ATPase Na+K+ transporting alpha-1 polypeptide-like; Hydrogen/potassium-exchanging ATPase 12A; Non-gastric H(+)/K(+) ATPase subunit alpha; Potassium-transporting ATPase alpha chain 2; Proton pump; Sodium/potassium ATPase alpha polypeptide-like;

Immunogens

Immunogen:
Uniprot:

P54707(AT12A_HUMAN) >>Visit HPA database.

Gene(ID):
ATP12A(479)
Expression:
P54707 AT12A_HUMAN:

Found in skin and kidney. Detected in prostate basal cells (at protein level). Expression is increased in benign prostate hyperplasia and tumor tissues (at protein level).

Sequence:
MHQKTPEIYSVELSGTKDIVKTDKGDGKEKYRGLKNNCLELKKKNHKEEFQKELHLDDHKLSNRELEEKYGTDIIMGLSSTRAAELLARDGPNSLTPPKQTPEIVKFLKQMVGGFSILLWVGAFLCWIAYGIQYSSDKSASLNNVYLGCVLGLVVILTGIFAYYQEAKSTNIMSSFNKMIPQQALVIRDSEKKTIPSEQLVVGDIVEVKGGDQIPADIRVLSSQGCRVDNSSLTGESEPQPRSSEFTHENPLETKNICFYSTTCLEGTVTGMVINTGDRTIIGHIASLASGVGNEKTPIAIEIEHFVHIVAGVAVSIGILFFIIAVSLKYQVLDSIIFLIGIIVANVPEGLLATVTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDNQIFVADTSEDHSNQVFDQSSRTWASLSKIITLCNRAEFKPGQENVPIMKKAVIGDASETALLKFSEVILGDVMEIRKRNRKVAEIPFNSTNKFQLSIHEMDDPHGKRFLMVMKGAPERILEKCSTIMINGEEHPLDKSTAKTFHTAYMELGGLGERVLGFCHLYLPADEFPETYSFDIDAMNFPTSNLCFVGLLSMIDPPRSTVPDAVTKCRSAGIKVIMVTGDHPITAKAIAKSVGIISANSETVEDIAHRLNIAVEQVNKRDAKAAVVTGMELKDMSSEQLDEILANYQEIVFARTSPQQKLIIVEGCQRQDAVVAVTGDGVNDSPALKKADIGIAMGIAGSDAAKNAADMVLLDDNFASIVTGVEEGRLIFDNLKKTIAYSLTKNIAELCPFLIYIIVGLPLPIGTITILFIDLGTDIIPSIALAYEKAESDIMNRKPRHKNKDRLVNQPLAVYSYLHIGLMQALGAFLVYFTVYAQEGFLPRTLINLRVEWEKDYVNDLKDSYGQEWTRYQREYLEWTGYTAFFVGILVQQIADLIIRKTRRNSIFQQGLFRNKVIWVGITSQIIIGLILSYGLGSVTALSFTMLRAQYWFVAVPHAILIWVYDEVRKLFIRLYPGSWWDKNMYY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
92
Chicken
92
Rabbit
92
Horse
83
Bovine
83
Dog
83
Xenopus
83
Pig
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P54707 As Substrate

Site PTM Type Enzyme
T5 Phosphorylation
S10 Phosphorylation
K42 Acetylation
S94 Phosphorylation
T101 Phosphorylation
S169 Phosphorylation
T170 Phosphorylation
T362 Phosphorylation
K449 Acetylation
K459 Acetylation
K627 Ubiquitination
T632 Phosphorylation
T638 Phosphorylation
K640 Ubiquitination
K713 Ubiquitination
K788 Ubiquitination
K856 Acetylation
S985 Phosphorylation
Y1038 Phosphorylation
Y1039 Phosphorylation

Research Backgrounds

Function:

Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in skin and kidney. Detected in prostate basal cells (at protein level). Expression is increased in benign prostate hyperplasia and tumor tissues (at protein level).

Subunit Structure:

Composed of two subunits: alpha (catalytic) and beta.

Family&Domains:

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

Research Fields

· Metabolism > Energy metabolism > Oxidative phosphorylation.

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