NDUFB6 Antibody - #DF2213

(2)  
Product: NDUFB6 Antibody
Catalog: DF2213
Description: Rabbit polyclonal antibody to NDUFB6
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Xenopus
Mol.Wt.: 15 kDa; 15kD(Calculated).
Uniprot: O95139
RRID: AB_2839444

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Xenopus(82%)
Clonality:
Polyclonal
Specificity:
NDUFB6 Antibody detects endogenous levels of total NDUFB6.
RRID:
AB_2839444
Cite Format: Affinity Biosciences Cat# DF2213, RRID:AB_2839444.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B17; CI; CI B17; CI-B17; Complex I mitochondrial respiratory chain B17 subunit; Complex I-B17; Complex IB17; MGC13675; NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 6; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6; NADH ubiquinone oxidoreductase B17 subunit; NADH ubiquinone oxidoreductase beta subunit 6; NADH-ubiquinone oxidoreductase B17 subunit; NDUB6_HUMAN; NDUFB6;

Immunogens

Immunogen:
Uniprot:

O95139(NDUB6_HUMAN) >>Visit HPA database.

Gene(ID):
NDUFB6(4712)
Description:
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone
Sequence:
MTGYTPDEKLRLQQLRELRRRWLKDQELSPREPVLPPQKMGPMEKFWNKFLENKSPWRKMVHGVYKKSIFVFTHVLVPVWIIHYYMKYHVSEKPYGIVEKKSRIFPGDTILETGEVIPPMKEFPDQHH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Xenopus
82
Bovine
73
Dog
73
Rabbit
73
Sheep
64
Chicken
64
Pig
55
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95139 As Substrate

Site PTM Type Enzyme
T2 Acetylation
T2 Phosphorylation
T5 Phosphorylation
K9 Ubiquitination
K24 Ubiquitination
S29 Phosphorylation
K49 Acetylation
K49 Ubiquitination
K54 Ubiquitination
S55 Phosphorylation
K66 Acetylation
K66 Ubiquitination
K67 Acetylation
K93 Ubiquitination
Y95 Phosphorylation
K100 Ubiquitination
T113 Phosphorylation

Research Backgrounds

Function:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein>Matrix side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Complex I is composed of 45 different subunits.

Family&Domains:

Belongs to the complex I NDUFB6 subunit family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

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