NDUFA10 Antibody - #DF2211

(2)  
Product: NDUFA10 Antibody
Catalog: DF2211
Description: Rabbit polyclonal antibody to NDUFA10
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig
Mol.Wt.: 41 kDa; 41kD(Calculated).
Uniprot: O95299
RRID: AB_2839442

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%)
Clonality:
Polyclonal
Specificity:
NDUFA10 Antibody detects endogenous levels of total NDUFA10.
RRID:
AB_2839442
Cite Format: Affinity Biosciences Cat# DF2211, RRID:AB_2839442.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CI 42KD; CI-42k; Complex I-42kD; MGC5103; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10 42kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial complex I 42kDa subunit; NADH ubiquinone oxidoreductase 42 kDa subunit mitochondrial precursor; NADH-ubiquinone oxidoreductase 42 kDa subunit; ndufa10l;

Immunogens

Immunogen:
Uniprot:

O95299(NDUAA_HUMAN) >>Visit HPA database.

Gene(ID):
NDUFA10(4705)
Description:
All lanes : Anti-NDUFA10 antibody [EPR12135] (ab174829) at 1/1000 dilution;Lane 1 : HepG2 cell lysate;Lane 2 : HeLa cell lysate;Lane 3 : Jurkat cell lysate;Lane 4 : Raji cell lysate;Lane 5 : Human fetal heart lysate;Lysates/proteins at 10 g per lane
Sequence:
MALRLLKLAATSASARVVAAGAQRVRGIHSSVQCKLRYGMWHFLLGDKASKRLTERSRVITVDGNICTGKGKLAKEIAEKLGFKHFPEAGIHYPDSTTGDGKPLATDYNGNCSLEKFYDDPRSNDGNSYRLQSWLYSSRLLQYSDALEHLLTTGQGVVLERSIFSDFVFLEAMYNQGFIRKQCVDHYNEVKSVTICDYLPPHLVIYIDVPVPEVQRRIQKKGDPHEMKITSAYLQDIENAYKKTFLPEMSEKCEVLQYSAREAQDSKKVVEDIEYLKFDKGPWLKQDNRTLYHLRLLVQDKFEVLNYTSIPIFLPEVTIGAHQTDRVLHQFRELPGRKYSPGYNTEVGDKWIWLK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
83
Horse
75
Bovine
67
Sheep
67
Chicken
67
Xenopus
58
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95299 As Substrate

Site PTM Type Enzyme
K48 Ubiquitination
S57 Phosphorylation
T61 Phosphorylation
K80 Acetylation
K80 Ubiquitination
K84 Ubiquitination
K102 Ubiquitination
K181 Ubiquitination
K221 Acetylation
T230 Phosphorylation
S231 Phosphorylation
Y233 Phosphorylation
Y241 Phosphorylation
K242 Acetylation
K242 Ubiquitination
K243 Acetylation
K243 Ubiquitination
S250 Phosphorylation
K252 Ubiquitination
K267 Acetylation
K268 Ubiquitination
Y275 Phosphorylation
K277 Ubiquitination
K280 Ubiquitination
K285 Ubiquitination
K338 Ubiquitination
T345 Phosphorylation
K350 Ubiquitination

Research Backgrounds

Function:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

PTMs:

Phosphorylation at Ser-250 by PINK1 is required for the binding and/or reduction of the complex I substrate ubiquinone.

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction.

Family&Domains:

Belongs to the complex I NDUFA10 subunit family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

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