GNA13 Antibody - #DF2204

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Product: GNA13 Antibody
Catalog: DF2204
Description: Rabbit polyclonal antibody to GNA13
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Rabbit, Chicken, Xenopus
Mol.Wt.: 44 kDa; 44kD(Calculated).
Uniprot: Q14344
RRID: AB_2839435

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Rabbit(100%), Chicken(91%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
GNA13 Antibody detects endogenous levels of total GNA13.
RRID:
AB_2839435
Cite Format: Affinity Biosciences Cat# DF2204, RRID:AB_2839435.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

G alpha 13; G alpha-13; G-protein subunit alpha-13; GNA13; GNA13_HUMAN; Guanine nucleotide binding protein (G protein) alpha 13; Guanine nucleotide binding protein alpha 13 subunit; Guanine nucleotide-binding protein subunit alpha-13;

Immunogens

Immunogen:
Uniprot:

Q14344(GNA13_HUMAN) >>Visit HPA database.

Gene(ID):
GNA13(10672)
Expression:
Q14344 GNA13_HUMAN:

Expressed in testis, including in Leydig cells and in the seminiferous epithelium, in differentiating cells from the spermatogonia to mature spermatozoa stages and round spermatids (at protein level). Expressed in 99.2% of spermatozoa from healthy individuals, but only in 28.6% of macrocephalic spermatozoa from infertile patients (at protein level).

Description:
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
Sequence:
MADFLPSRSVLSVCFPGCLLTSGEAEQQRKSKEIDKCLSREKTYVKRLVKILLLGAGESGKSTFLKQMRIIHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNSNQQHGDKMMSFDTRAPMAAQGMVETRVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPDYIPSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLVECFRNKRRDQQQKPLYHHFTTAINTENIRLVFRDVKDTILHDNLKQLMLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Xenopus
91
Chicken
91
Horse
0
Bovine
0
Sheep
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14344 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
K36 Ubiquitination
T43 Phosphorylation
Y44 Phosphorylation
S59 Phosphorylation
K61 Ubiquitination
K66 Ubiquitination
K105 Ubiquitination
K183 Ubiquitination
Y189 Phosphorylation
T203 Phosphorylation P17612 (PRKACA)
K204 Ubiquitination
K214 Ubiquitination
K219 Ubiquitination
S285 Phosphorylation
K363 Acetylation
K363 Ubiquitination
K372 Acetylation
K372 Ubiquitination

Research Backgrounds

Function:

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation.

PTMs:

Palmitoylation is critical for proper membrane localization and signaling.

Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.

Subcellular Location:

Cell membrane>Lipid-anchor. Melanosome. Cytoplasm. Nucleus.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Detected in the cytoplasm of Leydig cells and in the seminiferous epithelium, including differentiating cells from the spermatogonia to mature spermatozoa stages (PubMed:18703424). In round spermatids, also present in the nuclei (PubMed:18703424).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in testis, including in Leydig cells and in the seminiferous epithelium, in differentiating cells from the spermatogonia to mature spermatozoa stages and round spermatids (at protein level). Expressed in 99.2% of spermatozoa from healthy individuals, but only in 28.6% of macrocephalic spermatozoa from infertile patients (at protein level).

Subunit Structure:

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site (By similarity). Interacts with UBXD5. Interacts with HAX1. Interacts (in GTP-bound form) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane. Interacts with RGS22. Interacts (in GTP-bound form) with ARHGEF1. Interacts (in GTP-bound form) with ARHGEF11 (via RGS domain). Interacts (in GTP-bound form) with ARHGEF12 (via RGS domain). Interacts with CTNND1. Interacts with GASL2L2.

Family&Domains:

Belongs to the G-alpha family. G(12) subfamily.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Nervous system > Long-term depression.

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