GNA12 Antibody - #DF2203
| Product: | GNA12 Antibody |
| Catalog: | DF2203 |
| Description: | Rabbit polyclonal antibody to GNA12 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 44 kDa.; 44kD(Calculated). |
| Uniprot: | Q03113 |
| RRID: | AB_2839434 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF2203, RRID:AB_2839434.
Fold/Unfold
G alpha 12; G alpha-12; G-protein subunit alpha-12; gep; Gna12; GNA12_HUMAN; guanine nucleotide binding protein (G protein) alpha 12; Guanine nucleotide binding protein alpha 12 subunit; Guanine nucleotide-binding protein subunit alpha-12; MGC104623; MGC99644; NNX3; RMP; WUGSC:H_GS165O14.2;
Immunogens
- Q03113 GNA12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGVVRTLSRCLLPAEAGGARERRAGSGARDAEREARRRSRDIDALLARERRAVRRLVKILLLGAGESGKSTFLKQMRIIHGREFDQKALLEFRDTIFDNILKGSRVLVDARDKLGIPWQYSENEKHGMFLMAFENKAGLPVEPATFQLYVPALSALWRDSGIREAFSRRSEFQLGESVKYFLDNLDRIGQLNYFPSKQDILLARKATKGIVEHDFVIKKIPFKMVDVGGQRSQRQKWFQCFDGITSILFMVSSSEYDQVLMEDRRTNRLVESMNIFETIVNNKLFFNVSIILFLNKMDLLVEKVKTVSIKKHFPDFRGDPHRLEDVQRYLVQCFDRKRRNRSKPLFHHFTTAIDTENVRFVFHAVKDTILQENLKDIMLQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q03113 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S40 | Phosphorylation | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| Y121 | Phosphorylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| R235 | Methylation | Uniprot | |
| K376 | Ubiquitination | Uniprot |
Research Backgrounds
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins. Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating proinflammatory cytokine production. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation. Together with NAPA promotes CDH5 localization to plasma membrane. May play a role in the control of cell migration through the TOR signaling cascade.
Cell membrane>Lipid-anchor. Lateral cell membrane>Lipid-anchor. Cytoplasm.
Note: CDH1 enhances cell membrane localization.
G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site (By similarity). Interacts with UBXD5. Interacts (in GTP-bound form) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane. Interacts with RGS22. Interacts (via N-terminus) with NAPA; the interaction promotes CDH5 localization to plasma membrane. Interacts with CTNND1 (via N-terminus); the interaction regulates CDH1-mediated cell-cell adhesion. Interacts with PPP2R1A; the interaction promotes protein phosphatase 2A activation causing dephosphorylation of MAPT. Interacts (in GTP-bound form) with ARHGEF1 (By similarity). Interacts (in GTP-bound form) with ARHGEF11 (via RGS domain). Interacts (in GTP-bound form) with ARHGEF12 (via RGS domain).
Belongs to the G-alpha family. G(12) subfamily.
Research Fields
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Nervous system > Long-term depression.
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