MCF2L Antibody | Affinity Biosciences

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Western blot analysis of MCF2L expression in Molt-4 whole cell lysates.

Product:	MCF2L Antibody
Catalog:	DF2202
Description:	Rabbit polyclonal antibody to MCF2L
Application:	WB IHC
Reactivity:	Human, Rat
Mol.Wt.:	128kDa; 128kD(Calculated).
Uniprot:	O15068
RRID:	AB_2839433

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Clonality:

Polyclonal

Specificity:

MCF2L Antibody detects endogenous levels of total MCF2L.

RRID:

AB_2839433
Cite Format: Affinity Biosciences Cat# DF2202, RRID:AB_2839433.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ARHGEF 14; DBL''s big sister; DBS; FLJ12122; Guanine nucleotide exchange factor DBS; KIAA0362; MCF 2L; MCF.2 cell line derived transforming sequence like; MCF.2 transforming sequence-like; MCF2 like protein; MCF2 transforming sequence like protein; MCF2-transforming sequence-like protein; Mcf2l; MCF2L_HUMAN; OST; OST oncogene;

Immunogens

Immunogen:

Uniprot:

O15068(MCF2L_HUMAN) >>Visit HPA database.

Gene(ID):

MCF2L(23263)

Description:

Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42.

Sequence:

O15068 MCF2L_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFDCWRFILCKRPGSNSYSSPQRPNEAKKEETDHQIDVSDVIRLVQDTPEATAMATDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSGKAHVPRAHP

PTMs - O15068 As Substrate

O15068

Site	PTM Type	Source
T96	Phosphorylation	Uniprot
S177	Phosphorylation	Uniprot
K182	Ubiquitination	Uniprot
Y222	Phosphorylation	Uniprot
K364	Ubiquitination	Uniprot
S371	Phosphorylation	Uniprot
S396	Phosphorylation	Uniprot
S412	Phosphorylation	Uniprot
K456	Ubiquitination	Uniprot
S578	Phosphorylation	Uniprot
S582	Phosphorylation	Uniprot
S589	Phosphorylation	Uniprot
S592	Phosphorylation	Uniprot
S594	Phosphorylation	Uniprot
K608	Acetylation	Uniprot
S609	Phosphorylation	Uniprot
S620	Phosphorylation	Uniprot
Y784	Phosphorylation	Uniprot
S785	Phosphorylation	Uniprot
S893	Phosphorylation	Uniprot
T902	Phosphorylation	Uniprot
S965	Phosphorylation	Uniprot
T972	Phosphorylation	Uniprot
S975	Phosphorylation	Uniprot
T992	Phosphorylation	Uniprot
Y1000	Phosphorylation	Uniprot
T1007	Phosphorylation	Uniprot
S1033	Phosphorylation	Uniprot
S1034	Phosphorylation	Uniprot

Research Backgrounds

O15068_MCF2L_HUMAN

Function:

Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways (By similarity). Seems to lack activity with RAC1. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42.

Does not catalyze guanine nucleotide exchange on CDC42.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Cytoplasm. Endomembrane system.
Note: Interaction with membranes enriched in phosphoinositides is mediated by the CRAL-TRIO domain.

Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Subunit Structure:

Interacts with GTP-bound RAC1 (By similarity). Interacts with CDC42. Interacts with RHOA. Interacts with CCPG1, which results in specific inhibition of its exchange activity toward RHOA, but does not affect its activity on CDC42 (By similarity).

Family&Domains:

The CRAL-TRIO domain mediates interaction with various inositol phospholipids, such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P).

The DH domain is involved in interaction with CCPG1.

Belongs to the MCF2 family.

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MCF2L Antibody - #DF2202