TAB1 Antibody | Affinity Biosciences

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Western blot analysis of Hela whole cell lysates, using MAP3K7IP1 Antibody.

DF7471 at 1/100 staining Mouse kidney tissue by IHC-P.

Product:	TAB1 Antibody
Catalog:	DF7471
Description:	Rabbit polyclonal antibody to TAB1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	54kDa; 55kD(Calculated).
Uniprot:	Q15750
RRID:	AB_2839408

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

TAB1 Antibody detects endogenous levels of total TAB1.

RRID:

AB_2839408
Cite Format: Affinity Biosciences Cat# DF7471, RRID:AB_2839408.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2310012M03Rik; 3'-Tab1; MAP3K7IP 1; MAP3K7IP1; MGC57664; Mitogen activated protein kinase kinase kinase 7 interacting protein 1; Mitogen-activated protein kinase kinase kinase 7-interacting protein 1; TAB 1; TAB1; TAB1_HUMAN; TAK1 binding protein 1; TAK1-binding protein 1; TGF beta activated kinase 1 binding protein 1; TGF-beta activated kinase 1/MAP3K7 binding protein 1; TGF-beta-activated kinase 1 and MAP3K7-binding protein 1; TGF-beta-activated kinase 1-binding protein 1; Transforming growth factor beta activated kinase binding protein 1;

Immunogens

Immunogen:

Uniprot:

Q15750(TAB1_HUMAN) >>Visit HPA database.

Gene(ID):

TAB1(10454)

Expression:

Q15750 TAB1_HUMAN:

Ubiquitous.

Description:

The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Sequence:

Q15750 TAB1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAQRRSLLQSEQQPSWTDDLPLCHLSGVGSASNRSYSADGKGTESHPPEDSWLKFRSENNCFLYGVFNGYDGNRVTNFVAQRLSAELLLGQLNAEHAEADVRRVLLQAFDVVERSFLESIDDALAEKASLQSQLPEGVPQHQLPPQYQKILERLKTLEREISGGAMAVVAVLLNNKLYVANVGTNRALLCKSTVDGLQVTQLNVDHTTENEDELFRLSQLGLDAGKIKQVGIICGQESTRRIGDYKVKYGYTDIDLLSAAKSKPIIAEPEIHGAQPLDGVTGFLVLMSEGLYKALEAAHGPGQANQEIAAMIDTEFAKQTSLDAVAQAVVDRVKRIHSDTFASGGERARFCPRHEDMTLLVRNFGYPLGEMSQPTPSPAPAAGGRVYPVSVPYSSAQSTSKTSVTLSLVMPSQGQMVNGAHSASTLDEATPTLTNQSPTLTLQSTNTHTQSSSSSSDGGLFRSRPAHSLPPGEDGRVEPYVDFAEFYRLWSVDHGEQSVVTAP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Rabbit

100

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15750 As Substrate

Q15750

Site	PTM Type	Enzyme	Source
S7	Phosphorylation		Uniprot
S11	Phosphorylation		Uniprot
S16	Phosphorylation		Uniprot
K42	Ubiquitination		Uniprot
K150	Ubiquitination		Uniprot
Y179	Phosphorylation		Uniprot
T185	Phosphorylation		Uniprot
K192	Ubiquitination		Uniprot
K227	Ubiquitination		Uniprot
K229	Ubiquitination		Uniprot
K249	Ubiquitination		Uniprot
K262	Ubiquitination		Uniprot
K264	Ubiquitination		Uniprot
S339	Phosphorylation		Uniprot
T376	Phosphorylation		Uniprot
S378	Phosphorylation		Uniprot
S395	O-Glycosylation		Uniprot
S423	Phosphorylation	Q16539 (MAPK14)	Uniprot
T431	Phosphorylation	Q16539 (MAPK14)	Uniprot
S438	Phosphorylation	Q16539 (MAPK14)	Uniprot
T442	Phosphorylation		Uniprot
S452	Phosphorylation	O43318 (MAP3K7) , Q16539 (MAPK14)	Uniprot
S453	Phosphorylation	O43318 (MAP3K7) , Q16539 (MAPK14)	Uniprot
S456	Phosphorylation	Q16539 (MAPK14) , O43318 (MAP3K7)	Uniprot
S457	Phosphorylation	Q16539 (MAPK14) , O43318 (MAP3K7)	Uniprot
S469	Phosphorylation		Uniprot
Y481	Phosphorylation		Uniprot
Y488	Phosphorylation		Uniprot
S492	Phosphorylation		Uniprot

Research Backgrounds

Q15750_TAB1_HUMAN

Function:

May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis.

PTMs:

Monoubiquitinated. Deubiquitinated by Y.enterocolitica YopP.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with XIAP and BIRC7. Interacts with TRAF6 and MAP3K7; during IL-1 signaling. Identified in the TRIKA2 complex composed of MAP3K7, TAB1 and TAB2.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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TAB1 Antibody - #DF7471