Product: SLC3A2 Antibody
Catalog: DF7468
Description: Rabbit polyclonal antibody to SLC3A2
Application: WB IHC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 67kDa; 68kD(Calculated).
Uniprot: P08195
RRID: AB_2839405

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SLC3A2 Antibody detects endogenous levels of total SLC3A2.
RRID:
AB_2839405
Cite Format: Affinity Biosciences Cat# DF7468, RRID:AB_2839405.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

4F2; 4F2 cell surface antigen heavy chain; 4F2 cell-surface antigen heavy chain; 4F2 heavy chain; 4F2 heavy chain antigen; 4F2_HUMAN; 4F2hc; 4T2HC; Antigen defined by monoclonal antibody 4F2 heavy chain; Antigen identified by monoclonal antibodies 4F2 TRA1.10 TROP4 and T43; CD 98; CD98; CD98 antigen; CD98 heavy chain; CD98HC; Heavy chain; Lymphocyte activation antigen 4F2 large subunit; MDU 1; MDU1; Monoclonal antibody 44D7; NACAE; Slc3a2; Solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2; Solute carrier family 3 member 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P08195 4F2_HUMAN:

Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1.

Description:
This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.
Sequence:
MELQPPEASIAVVSIPRQLPGSHSEAGVQGLSAGDDSELGSHCVAQTGLELLASGDPLPSASQNAEMIETGSDCVTQAGLQLLASSDPPALASKNAEVTGTMSQDTEVDMKEVELNELEPEKQPMNAASGAAMSLAGAEKNGLVKIKVAEDEAEAAAAAKFTGLSKEELLKVAGSPGWVRTRWALLLLFWLGWLGMLAGAVVIIVRAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Horse
92
Bovine
92
Sheep
92
Xenopus
75
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08195 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S9 Phosphorylation
S41 Phosphorylation
S103 Phosphorylation
T106 Phosphorylation
K111 Ubiquitination
K122 Ubiquitination
S129 Phosphorylation
S134 Phosphorylation
K140 Ubiquitination
K145 Ubiquitination
K147 Ubiquitination
K160 Ubiquitination
T162 Phosphorylation
S165 Phosphorylation
K166 Acetylation
K166 Sumoylation
K166 Ubiquitination
K171 Ubiquitination
S175 Phosphorylation
T181 Phosphorylation
K217 Ubiquitination
K245 Ubiquitination
Y250 Phosphorylation
K255 Ubiquitination
K257 Ubiquitination
K266 Ubiquitination
K269 Ubiquitination
K287 Ubiquitination
S292 Phosphorylation
S296 Phosphorylation
K298 Ubiquitination
S301 Phosphorylation
T309 Phosphorylation
Y312 Phosphorylation
S317 Phosphorylation
S320 Phosphorylation
T321 Phosphorylation
K329 Ubiquitination
K354 Ubiquitination
N365 N-Glycosylation
K368 Ubiquitination
N381 N-Glycosylation
S402 Phosphorylation
S403 Phosphorylation
Y404 Phosphorylation
S406 Phosphorylation P68400 (CSNK2A1)
S408 Phosphorylation P68400 (CSNK2A1)
S410 Phosphorylation P68400 (CSNK2A1)
K416 Ubiquitination
N424 N-Glycosylation
T442 Phosphorylation
S443 Phosphorylation
N506 N-Glycosylation
S513 Phosphorylation
S518 Phosphorylation
S521 Phosphorylation
S527 Phosphorylation P68400 (CSNK2A1)
S531 Phosphorylation P68400 (CSNK2A1)
S598 Phosphorylation
S607 Phosphorylation
K615 Ubiquitination
R625 Methylation

Research Backgrounds

Function:

Component of several heterodimeric amino acid transporter complexes. The precise substrate specificity depends on the other subunit in the heterodimer. The heterodimer with SLC3A2 functions as sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, L-DOPA, leucine, histidine, methionine and tryptophan. The complexes with SLC7A6 and SLC7A7 mediate uptake of dibasic amino acids. The complexes function as amino acid exchangers. Required for targeting of SLC7A5 and SLC7A8 to the plasma membrane and for channel activity. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. The heterodimer with SLC7A5/LAT1 may play a role in the transport of L-DOPA across the blood-brain barrier (By similarity). May mediate blood-to-retina L-leucine transport across the inner blood-retinal barrier (By similarity). The heterodimer with SLC7A5/LAT1 can mediate the transport of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. The heterodimer with SLC7A5 is involved in the uptake of toxic methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes. Together with ICAM1, regulates the transport activity SLC7A8 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with LAPTM4B, the heterodimer formed by SLC3A2 and SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation.

PTMs:

Phosphorylation on Ser-406; Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions.

Subcellular Location:

Apical cell membrane. Cell membrane>Single-pass type II membrane protein. Cell junction. Lysosome membrane. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Localized at the plasma membrane when associated with SLC7A5 or SLC7A8 (PubMed:9751058, PubMed:11311135). Localized to the apical membrane of placental syncytiotrophoblastic cells (PubMed:11742812). Recruited to lysosomes by LAPTM4B (PubMed:25998567). Located selectively at cell-cell adhesion sites (By similarity). Colocalized with SLC7A8/LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1.

Subunit Structure:

Disulfide-linked heterodimer with a non-glycosylated light chain (SLC7A5, SLC7A6, SLCA7A7, SLC7A8, SLC7A10 or SLCA7A11). Interacts with TLCD3A/CT120 and ICAM1. Constitutively and specifically associates with beta-1 integrins (alpha-2/beta-1, alpha-3/beta-1, alpha-5/beta-1 and alpha-6/beta-1), but minimally with alpha-4/beta-1. Interacts with LAPTM4B; recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation.

Family&Domains:

Belongs to the SLC3A transporter family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Organismal Systems > Digestive system > Protein digestion and absorption.

References

1). The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11. European Journal of Pharmacology, 2022 (PubMed: 35700835) [IF=5.0]

2). Identification and analysis of a prognostic ferroptosis and iron-metabolism signature for esophageal squamous cell carcinoma. Journal of Cancer, 2023 (PubMed: 35371305) [IF=3.9]

Application: IF/ICC    Species: Human    Sample: ESCC

Figure 4 Immunofluorescence of PRNP (green), SLC39A8 (red), LCN2 (red), ATP6V0A1 (green), SLC3A2 (red), and SLC39A14 (green) in ESCC samples and normal tissues.

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