GSTP1 Antibody - #DF7462

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Product: GSTP1 Antibody
Catalog: DF7462
Description: Rabbit polyclonal antibody to GSTP1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Rabbit, Dog, Xenopus
Mol.Wt.: 23kDa; 23kD(Calculated).
Uniprot: P09211
RRID: AB_2839399

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Bovine(100%), Rabbit(100%), Dog(100%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
GSTP1 Antibody detects endogenous levels of total GSTP1.
RRID:
AB_2839399
Cite Format: Affinity Biosciences Cat# DF7462, RRID:AB_2839399.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Deafness X linked 7; DFN7; FAEES3; Fatty acid ethyl ester synthase III; Glutathione S transferase pi 1; Glutathione S-transferase P; GST class-pi; GST3; Gstp1; GSTP1-1; GSTP1_HUMAN; GSTpi; PI;

Immunogens

Immunogen:
Uniprot:

P09211(GSTP1_HUMAN) >>Visit HPA database.

Gene(ID):
GSTP1(2950)
Description:
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
Sequence:
MPPYTVVYFPVRGRCAALRMLLADQGQSWKEEVVTVETWQEGSLKASCLYGQLPKFQDGDLTLYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYISLIYTNYEAGKDDYVKALPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVGRLSARPKLKAFLASPEYVNLPINGNGKQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Rabbit
100
Pig
89
Xenopus
89
Horse
0
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09211 As Substrate

Site PTM Type Enzyme
Y4 Phosphorylation P00533 (EGFR)
Y8 Phosphorylation P00533 (EGFR)
S28 Phosphorylation
K30 Sumoylation
S43 Phosphorylation P24723 (PRKCH)
K45 Methylation
S47 Phosphorylation
C48 S-Nitrosylation
Y50 Phosphorylation
K55 Acetylation
K55 Ubiquitination
T62 Phosphorylation
Y64 Phosphorylation
Y80 Phosphorylation
K82 Methylation
K82 Sumoylation
K82 Ubiquitination
K103 Acetylation
K103 Ubiquitination
S106 Phosphorylation
Y109 Phosphorylation
T110 Phosphorylation
Y112 Phosphorylation
K116 Acetylation
K121 Acetylation
K121 Ubiquitination
K128 Acetylation
K128 Ubiquitination
T132 Phosphorylation
S135 Phosphorylation
K141 Methylation
K141 Sumoylation
S185 Phosphorylation P24723 (PRKCH) , P17252 (PRKCA) , P17612 (PRKACA)
K189 Acetylation
K189 Ubiquitination
K191 Acetylation
K191 Ubiquitination
S196 Phosphorylation
Y199 Phosphorylation P00533 (EGFR)
K209 Acetylation
K209 Methylation
K209 Ubiquitination

Research Backgrounds

Function:

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.

Subcellular Location:

Cytoplasm. Mitochondrion. Nucleus.
Note: The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Interacts with CDK5.

Family&Domains:

Belongs to the GST superfamily. Pi family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

References

1). Reconstructing hepatic metabolic profile and glutathione-mediated metabolic fate of acrylamide. Environmental Pollution, 2023 (PubMed: 37673322) [IF=8.9]
2). Effect of verapamil in the reversal of doxorubicin chemotherapy resistance in advanced gastric cancer. European Review for Medical and Pharmacological Sciences, 2020 (PubMed: 32744702) [IF=3.3]

Application: WB    Species: human    Sample: GC cells

Figure 2. |Evaluation of candidate gene/protein expression in GC cells. A, RT-qPCR detection of candidate gene expression in GC cells. Independent experiments were performed during the whole in vitro study. B, Western blot analysis of candidate protein expression levels in GC cells.

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