MSH3 Antibody - #DF7457

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Product: MSH3 Antibody
Catalog: DF7457
Description: Rabbit polyclonal antibody to MSH3
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Horse, Sheep, Dog
Mol.Wt.: 127kDa; 127kD(Calculated).
Uniprot: P20585
RRID: AB_2839394

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Dog(88%)
Clonality:
Polyclonal
Specificity:
MSH3 Antibody detects endogenous levels of total MSH3.
RRID:
AB_2839394
Cite Format: Affinity Biosciences Cat# DF7457, RRID:AB_2839394.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Divergent upstream protein; DNA mismatch repair protein; DNA mismatch repair protein Msh 3; DNA mismatch repair protein MSH3; DUC 1; DUC1; DUG; DUP; hMSH3; MGC163306; MGC163308; Mismatch repair protein 1; MRP 1; MRP1; MSH 3; MSH3; MSH3_HUMAN; MutS homolog 3 (E. coli); MutS homolog 3;

Immunogens

Immunogen:
Uniprot:

P20585(MSH3_HUMAN) >>Visit HPA database.

Gene(ID):
MSH3(4437)
Description:
The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer.
Sequence:
MSRRKPASGGLAASSSAPARQAVLSRFFQSTGSLKSTSSSTGAADQVDPGAAAAAAAAAAAAPPAPPAPAFPPQLPPHIATEIDRRKKRPLENDGPVKKKVKKVQQKEGGSDLGMSGNSEPKKCLRTRNVSKSLEKLKEFCCDSALPQSRVQTESLQERFAVLPKCTDFDDISLLHAKNAVSSEDSKRQINQKDTTLFDLSQFGSSNTSHENLQKTASKSANKRSKSIYTPLELQYIEMKQQHKDAVLCVECGYKYRFFGEDAEIAARELNIYCHLDHNFMTASIPTHRLFVHVRRLVAKGYKVGVVKQTETAALKAIGDNRSSLFSRKLTALYTKSTLIGEDVNPLIKLDDAVNVDEIMTDTSTSYLLCISENKENVRDKKKGNIFIGIVGVQPATGEVVFDSFQDSASRSELETRMSSLQPVELLLPSALSEQTEALIHRATSVSVQDDRIRVERMDNIYFEYSHAFQAVTEFYAKDTVDIKGSQIISGIVNLEKPVICSLAAIIKYLKEFNLEKMLSKPENFKQLSSKMEFMTINGTTLRNLEILQNQTDMKTKGSLLWVLDHTKTSFGRRKLKKWVTQPLLKLREINARLDAVSEVLHSESSVFGQIENHLRKLPDIERGLCSIYHKKCSTQEFFLIVKTLYHLKSEFQAIIPAVNSHIQSDLLRTVILEIPELLSPVEHYLKILNEQAAKVGDKTELFKDLSDFPLIKKRKDEIQGVIDEIRMHLQEIRKILKNPSAQYVTVSGQEFMIEIKNSAVSCIPTDWVKVGSTKAVSRFHSPFIVENYRHLNQLREQLVLDCSAEWLDFLEKFSEHYHSLCKAVHHLATVDCIFSLAKVAKQGDYCRPTVQEERKIVIKNGRHPVIDVLLGEQDQYVPNNTDLSEDSERVMIITGPNMGGKSSYIKQVALITIMAQIGSYVPAEEATIGIVDGIFTRMGAADNIYKGQSTFMEELTDTAEIIRKATSQSLVILDELGRGTSTHDGIAIAYATLEYFIRDVKSLTLFVTHYPPVCELEKNYSHQVGNYHMGFLVSEDESKLDPGAAEQVPDFVTFLYQITRGIAARSYGLNVAKLADVPGEILKKAAHKSKELEGLINTKRKRLKYFAKLWTMHNAQDLQKWTEEFNMEETQTSLLH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
88
Rabbit
75
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20585 As Substrate

Site PTM Type Enzyme
S14 Phosphorylation
S16 Phosphorylation
S30 Phosphorylation
S33 Phosphorylation
K103 Acetylation
K107 Ubiquitination
S111 Phosphorylation
S116 Phosphorylation
K165 Ubiquitination
K178 Ubiquitination
K193 Ubiquitination
S201 Phosphorylation
K215 Ubiquitination
Y229 Phosphorylation
Y236 Phosphorylation
K240 Ubiquitination
K308 Ubiquitination
K316 Methylation
K316 Ubiquitination
S337 Phosphorylation
T338 Phosphorylation
S520 Phosphorylation
T540 Phosphorylation
T541 Phosphorylation
K557 Sumoylation
K586 Ubiquitination
K695 Ubiquitination
K704 Ubiquitination
K713 Ubiquitination
K770 Ubiquitination
K775 Ubiquitination
K842 Ubiquitination
K902 Ubiquitination
T967 Phosphorylation
S968 Phosphorylation
T1054 Phosphorylation
Y1068 Phosphorylation
K1074 Ubiquitination
K1091 Ubiquitination
T1099 Phosphorylation
K1100 Ubiquitination

Research Backgrounds

Function:

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

Subunit Structure:

Component of the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Interacts with MCM9.

Family&Domains:

Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.

Research Fields

· Genetic Information Processing > Replication and repair > Mismatch repair.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

Restrictive clause

 

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