CD10 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using CD10 Antibody at 1/1000 dilution.

DF7446 at 1/100 staining Mouse colorectal tissue by IHC-P.

DF7446 at 1/100 staining Rat kidney tissue by IHC-P.

DF7446 staining Hela cells by IF/ICC.

Product:	CD10 Antibody
Catalog:	DF7446
Description:	Rabbit polyclonal antibody to CD10
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	85kDa; 86kD(Calculated).
Uniprot:	P08473
RRID:	AB_2839384

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(91%)

Clonality:

Polyclonal

Specificity:

CD10 Antibody detects endogenous levels of total CD10.

RRID:

AB_2839384
Cite Format: Affinity Biosciences Cat# DF7446, RRID:AB_2839384.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Atriopeptidase; CALLA; CD10; CD10 antigen; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24.11; Enkephalinase; EPN; Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase); Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); Membrane metallo endopeptidase; Membrane metallo endopeptidase variant 1; Membrane metallo endopeptidase variant 2; Membrane metalloendopeptidase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10; Membrane metalloendopeptidase variant 1; Membrane metalloendopeptidase variant 2; MGC126681; MGC126707; MME; NEP; NEP_HUMAN; Neprilysin; neprilysin-390; neprilysin-411; Neutral endopeptidase 24.11; Neutral endopeptidase; Neutral endopeptidase, membrane-associated; SFE; Skin fibroblast elastase;

Immunogens

Immunogen:

Uniprot:

P08473(NEP_HUMAN) >>Visit HPA database.

Gene(ID):

MME(4311)

Description:

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.

Sequence:

P08473 NEP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGKSESQMDITDINTPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Dog

100

Rabbit

100

Horse

Sheep

Chicken

Pig

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08473 As Substrate

P08473

Site	PTM Type	Enzyme	Source
G2	Myristoylation		Uniprot
S4	Phosphorylation		Uniprot
S6	Phosphorylation	P68400 (CSNK2A1) , P67870 (CSNK2B)	Uniprot
T11	Phosphorylation		Uniprot
T15	Phosphorylation		Uniprot
T25	Phosphorylation		Uniprot
K85	Acetylation		Uniprot
K93	Acetylation		Uniprot
N145	N-Glycosylation		Uniprot
T207	Phosphorylation		Uniprot
N285	N-Glycosylation		Uniprot
N325	N-Glycosylation		Uniprot
Y387	Phosphorylation		Uniprot
Y399	Phosphorylation		Uniprot
T407	Phosphorylation		Uniprot
Y482	Phosphorylation		Uniprot
Y497	Phosphorylation		Uniprot
Y502	Phosphorylation		Uniprot
Y507	Phosphorylation		Uniprot
N628	N-Glycosylation		Uniprot
S713	Phosphorylation		Uniprot

Research Backgrounds

P08473_NEP_HUMAN

Function:

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

PTMs:

Myristoylation is a determinant of membrane targeting.

Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity.

Subcellular Location:

Cell membrane>Single-pass type II membrane protein.

Family&Domains:

Belongs to the peptidase M13 family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Organismal Systems > Endocrine system > Renin-angiotensin system. (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

· Organismal Systems > Digestive system > Protein digestion and absorption.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CD10 Antibody - #DF7446