FLI1 Antibody - #DF7434

(2)  
Product: FLI1 Antibody
Catalog: DF7434
Description: Rabbit polyclonal antibody to FLI1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 50kDa; 51kD(Calculated).
Uniprot: Q01543
RRID: AB_2839372

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
FLI1 Antibody detects endogenous levels of total FLI1.
RRID:
AB_2839372
Cite Format: Affinity Biosciences Cat# DF7434, RRID:AB_2839372.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ERGB transcription factor; Ewing Sarcoma breakpoint region 2; EWSR 2; EWSR2; FLI 1; FLI 1 proto oncogene; Fli-1 proto-oncogene, ETS transcription factor; FLI1; FLI1 EWS fusion gene; FLI1 proto oncogene; FLI1_HUMAN; Friend leukemia integration 1 (FLI1) transcription factor; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; Sarcoma breakpoint region 2 (EWSR2); SIC 1; SIC1; Transcription factor ERGB; Viral integration region FLI1; Viral integration region FLI1, mouse, homolog of;

Immunogens

Immunogen:
Uniprot:

Q01543(FLI1_HUMAN) >>Visit HPA database.

Gene(ID):
FLI1(2313)
Description:
This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.
Sequence:
MDGTIKEALSVVSDDQSLFDSAYGAAAHLPKADMTASGSPDYGQPHKINPLPPQQEWINQPVRVNVKREYDHMNGSRESPVDCSVSKCSKLVGGGESNPMNYNSYMDEKNGPPPPNMTTNERRVIVPADPTLWTQEHVRQWLEWAIKEYSLMEIDTSFFQNMDGKELCKMNKEDFLRATTLYNTEVLLSHLSYLRESSLLAYNTTSHTDQSSRLSVKEDPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYHAHQQKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVPSHLGSYY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01543 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
S13 Phosphorylation
S21 Phosphorylation
T35 Phosphorylation
S37 Phosphorylation
S39 Phosphorylation
Y42 Phosphorylation
K47 Ubiquitination
K67 Sumoylation
Y70 Phosphorylation
S76 Phosphorylation
S79 Phosphorylation
S86 Phosphorylation
K87 Ubiquitination
S89 Phosphorylation
K172 Ubiquitination
K217 Ubiquitination
S221 Phosphorylation
Y222 Phosphorylation
S224 Phosphorylation
S236 Phosphorylation
K240 Acetylation
K240 Ubiquitination
S241 Phosphorylation
K252 Acetylation
Y263 Phosphorylation
T312 Phosphorylation Q05655 (PRKCD)
K325 Ubiquitination
Y332 Phosphorylation
K334 Ubiquitination
K350 Ubiquitination
K380 Acetylation
K380 Ubiquitination
K397 Acetylation
R438 Methylation
S450 Phosphorylation
Y451 Phosphorylation
Y452 Phosphorylation

Research Backgrounds

Function:

Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Can form homodimers or heterodimers with ETV6/TEL1.

Family&Domains:

Belongs to the ETS family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.