CREM Antibody - #DF7423

(2)  
Product: CREM Antibody
Catalog: DF7423
Description: Rabbit polyclonal antibody to CREM
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 38kDa; 37kD(Calculated).
Uniprot: Q03060
RRID: AB_2839361

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
CREM Antibody detects endogenous levels of total CREM.
RRID:
AB_2839361
Cite Format: Affinity Biosciences Cat# DF7423, RRID:AB_2839361.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cAMP response element modulator; cAMP responsive element modulator; cAMP-responsive element modulator; CREM; CREM-2; CREM_HUMAN; hCREM 2; hCREM-2; hCREM2; ICER; Inducible cAMP early repressor; Inducible cAMP early repressor ICER; MGC111110; MGC17881; MGC41893;

Immunogens

Immunogen:
Uniprot:

Q03060(CREM_HUMAN) >>Visit HPA database.

Gene(ID):
CREM(1390)
Expression:
Q03060 CREM_HUMAN:

Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.

Description:
This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription.
Sequence:
MTMETVESQHDGSITASLTESKSAHVQTQTGQNSIPALAQVSVAGSGTRRGSPAVTLVQLPSGQTIHVQGVIQTPQPWVIQSSEIHTVQVAAIAETDESAESEGVIDSHKRREILSRRPSYRKILNELSSDVPGVPKIEEERSEEEGTPPSIATMAVPTSIYQTSTGQYIAIAQGGTIQISNPGSDGVQGLQALTMTNSGAPPPGATIVQYAAQSADGTQQFFVPGSQVVVQDEETELAPSHMAAATGDMPTYQIRAPTAALPQGVVMAASPGSLHSPQQLAEEATRKRELRLMKNREAAKECRRRKKEYVKCLESRVAVLEVQNKKLIEELETLKDICSPKTDY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q03060 As Substrate

Site PTM Type Enzyme
S42 Phosphorylation
S108 Phosphorylation
S116 Phosphorylation
S120 Phosphorylation
Y121 Phosphorylation
S129 Phosphorylation
T148 Phosphorylation
T259 Phosphorylation
S271 Phosphorylation
S274 Phosphorylation
S277 Phosphorylation
T286 Phosphorylation

Research Backgrounds

Function:

Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation.

May play a role in the regulation of the circadian clock: acts as a transcriptional repressor of the core circadian component PER1 by directly binding to cAMP response elements in its promoter.

PTMs:

Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome.

Stimulated by phosphorylation. Phosphorylated on Ser-116 by TSSK4 in vitro.

Subcellular Location:

Nucleus.

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.

Subunit Structure:

Binds DNA as a dimer. Interacts with FHL5 (By similarity). Interacts with CDC34. May interact with TSSK4 (By similarity).

Family&Domains:

Belongs to the bZIP family.

Research Fields

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

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