PGRMC1 Antibody - #DF7422

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Product: PGRMC1 Antibody
Catalog: DF7422
Description: Rabbit polyclonal antibody to PGRMC1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 21kDa; 22kD(Calculated).
Uniprot: O00264
RRID: AB_2839360

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(89%)
Clonality:
Polyclonal
Specificity:
PGRMC1 Antibody detects endogenous levels of total PGRMC1.
RRID:
AB_2839360
Cite Format: Affinity Biosciences Cat# DF7422, RRID:AB_2839360.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HPR6.6; Membrane associated progesterone receptor component 1; Membrane-associated progesterone receptor component 1; mPR; PGRC1_HUMAN; PGRMC; Pgrmc1; Progesterone binding protein; Progesterone receptor membrane component 1;

Immunogens

Immunogen:

A synthesized peptide derived from human PGRMC1, corresponding to a region within C-terminal amino acids.

Uniprot:

O00264(PGRC1_HUMAN) >>Visit HPA database.

Gene(ID):
PGRMC1(10857)
Expression:
O00264 PGRC1_HUMAN:

Widely expressed, with highest expression in liver and kidney.

Description:
This gene encodes a putative membrane-associated progesterone steroid receptor. The protein is expressed predominantly in the liver and kidney.
Sequence:
MAAEDVVATGADPSDLESGGLLHEIFTSPLNLLLLGLCIFLLYKIVRGDQPAASGDSDDDEPPPLPRLKRRDFTPAELRRFDGVQDPRILMAINGKVFDVTKGRKFYGPEGPYGVFAGRDASRGLATFCLDKEALKDEYDDLSDLTAAQQETLSDWESQFTFKYHHVGKLLKEGEEPTVYSDEEEPKDESARKND

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
89
Bovine
89
Sheep
89
Rabbit
89
Dog
78
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Component of a progesterone-binding protein complex. Binds progesterone. Has many reported cellular functions (heme homeostasis, interaction with CYPs).

Subcellular Location:

Microsome membrane>Single-pass membrane protein. Smooth endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, with highest expression in liver and kidney.

Family&Domains:

The cytochrome b5 heme-binding domain lacks the conserved iron-binding His residues at positions 107 and 131.

Belongs to the cytochrome b5 family. MAPR subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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