Thymidine Kinase 1/TK1 Antibody - #DF7416
(1)
| Product: | Thymidine Kinase 1/TK1 Antibody |
| Catalog: | DF7416 |
| Description: | Rabbit polyclonal antibody to Thymidine Kinase 1/TK1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 25kDa; 25kD(Calculated). |
| Uniprot: | P04183 |
| RRID: | AB_2839354 |
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
Thymidine Kinase 1/TK1 Antibody detects endogenous levels of total Thymidine Kinase 1/TK1.
RRID:
AB_2839354
Cite Format: Affinity Biosciences Cat# DF7416, RRID:AB_2839354.
Cite Format: Affinity Biosciences Cat# DF7416, RRID:AB_2839354.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
cytosolic; KITH_HUMAN; Thymidine kinase 1; Thymidine kinase 1 soluble; Thymidine kinase 1 soluble isoform; Thymidine kinase; Thymidine kinase cytosolic; TK 1; TK 2; TK1; Tk1a; Tk1b; TK2;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- P04183 KITH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPAN
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
88
Zebrafish
86
Horse
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P04183 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| S15 | Phosphorylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| K32 | Acetylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| Y55 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| R70 | Methylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K164 | Acetylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| Y181 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K220 | Acetylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| C230 | S-Nitrosylation | Uniprot | |
| S231 | Phosphorylation | Uniprot |
Research Backgrounds
PTMs:
Phosphorylated on Ser-13 in mitosis.
Subcellular Location:
Cytoplasm.
Subunit Structure:
Homotetramer.
Family&Domains:
Belongs to the thymidine kinase family.
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For Research Use Only.
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