WHSC1L1 Antibody - #DF7410

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Product: WHSC1L1 Antibody
Catalog: DF7410
Description: Rabbit polyclonal antibody to WHSC1L1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 161kDa, 200 kDa; 162kD(Calculated).
Uniprot: Q9BZ95
RRID: AB_2839348

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(92%), Horse(83%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
WHSC1L1 Antibody detects endogenous levels of total WHSC1L1.
RRID:
AB_2839348
Cite Format: Affinity Biosciences Cat# DF7410, RRID:AB_2839348.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp667H044; FLJ20353; Histone lysine N methyltransferase NSD3; Histone-lysine N-methyltransferase NSD3; MGC126766; MGC142029; NSD 3; NSD3_HUMAN; Nuclear set domain containing 3; Nuclear SET domain containing protein 3; Nuclear SET domain-containing protein 3; pp14328; Protein whistle; Whistle; WHSC1 like protein 1; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; WHSC1-like protein 1; WHSC1L1; WHSC1L1 protein; Wolf Hirschhorn syndrome candidate 1 like 1; Wolf-Hirschhorn syndrome candidate 1-like protein 1;

Immunogens

Immunogen:
Uniprot:

Q9BZ95(NSD3_HUMAN) >>Visit HPA database.

Gene(ID):
NSD3(54904)
Expression:
Q9BZ95 NSD3_HUMAN:

Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.

Description:
This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described.
Sequence:
MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIAEDGGQTPYEATLQQGFQYPATTEDLPPLTNGYPSSISVYETQTKYQSYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEERKSHKIPKLEPEEQNRPNERVDTVSEKPREEPVLKEEAPVQPILSSVPTTEVSTGVKFQVGDLVWSKVGTYPWWPCMVSSDPQLEVHTKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHKQYEELLAEATKQASNHSEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERIEQYTFIYIDKQPEEALSQAKKSVASKTEVKKTRRPRSVLNTQPEQTNAGEVASSLSSTEIRRHSQRRHTSAEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKPTQSVSSPEATSGSTGSVEKKQQRRSIRTRSESEKSTEVVPKKKIKKEQVETVPQATVKTGLQKGASEISDSCKPLKKRSRASTDVEMTSSAYRDTSDSDSRGLSDLQVGFGKQVDSPSATADADVSDVQSMDSSLSRRGTGMSKKDTVCQICESSGDSLIPCEGECCKHFHLECLGLASLPDSKFICMECKTGQHPCFSCKVSGKDVKRCSVGACGKFYHEACVRKFPTAIFESKGFRCPQHCCSACSMEKDIHKASKGRMMRCLRCPVAYHSGDACIAAGSMLVSSYILICSNHSKRSSNSSAVNVGFCFVCARGLIVQDHSDPMFSSYAYKSHYLLNESNRAELMKLPMIPSSSASKKKCEKGGRLLCCESCPASFHPECLSIEMPEGCWNCNDCKAGKKLHYKQIVWVKLGNYRWWPAEICNPRSVPLNIQGLKHDLGDFPVFFFGSHDYYWVHQGRVFPYVEGDKSFAEGQTSINKTFKKALEEAAKRFQELKAQRESKEALEIEKNSRKPPPYKHIKANKVIGKVQIQVADLSEIPRCNCKPADENPCGLESECLNRMLQYECHPQVCPAGDRCQNQCFTKRLYPDAEIIKTERRGWGLRTKRSIKKGEFVNEYVGELIDEEECRLRIKRAHENSVTNFYMLTVTKDRIIDAGPKGNYSRFMNHSCNPNCETQKWTVNGDVRVGLFALCDIPAGMELTFNYNLDCLGNGRTECHCGADNCSGFLGVRPKSACASTNEEKAKNAKLKQKRRKIKTEPKQMHEDYCFQCGDGGELVMCDKKDCPKAYHLLCLNLTQPPYGKWECPWHQCDECSSAAVSFCEFCPHSFCKDHEKGALVPSALEGRLCCSEHDPMAPVSPEYWSKIKCKWESQDHGEEVKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Rabbit
100
Bovine
92
Horse
83
Xenopus
67
Chicken
55
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BZ95 As Substrate

Site PTM Type Enzyme
S107 Phosphorylation
K127 Ubiquitination
S129 Phosphorylation
S150 Phosphorylation
K159 Ubiquitination
S183 Phosphorylation
T186 Phosphorylation
K187 Ubiquitination
S188 Phosphorylation
K218 Sumoylation
K245 Sumoylation
K334 Ubiquitination
K345 Ubiquitination
Y390 Phosphorylation
Y394 Phosphorylation
S424 Phosphorylation
T428 Phosphorylation
T433 Phosphorylation
T456 Phosphorylation
S457 Phosphorylation
K467 Sumoylation
K467 Ubiquitination
K471 Ubiquitination
K477 Methylation
K477 Ubiquitination
S478 Phosphorylation
K487 Ubiquitination
S489 Phosphorylation
K494 Ubiquitination
S498 Phosphorylation
Y524 Phosphorylation
K527 Ubiquitination
K532 Acetylation
K532 Sumoylation
K532 Ubiquitination
S546 Phosphorylation
T547 Phosphorylation
K554 Acetylation
K554 Ubiquitination
T556 Phosphorylation
S558 Phosphorylation
S560 Phosphorylation
S561 Phosphorylation
S568 Phosphorylation
T569 Phosphorylation
S571 Phosphorylation
S580 Phosphorylation
T583 Phosphorylation
S585 Phosphorylation
S587 Phosphorylation
S590 Phosphorylation
K601 Sumoylation
T611 Phosphorylation
K628 Sumoylation
S634 Phosphorylation
S637 Phosphorylation
T638 Phosphorylation
S655 Phosphorylation
S659 Phosphorylation
K667 Methylation
S671 Phosphorylation
K781 Acetylation
K790 Acetylation
S889 Phosphorylation
Y891 Phosphorylation
S896 Phosphorylation
K914 Acetylation
K915 Acetylation
Y960 Phosphorylation
Y971 Phosphorylation
K1058 Sumoylation
K1065 Ubiquitination
Y1073 Phosphorylation
K1074 Ubiquitination
K1077 Acetylation
K1080 Acetylation
K1141 Ubiquitination
K1151 Sumoylation
K1167 Ubiquitination
K1215 Ubiquitination
K1391 Ubiquitination
S1406 Phosphorylation
S1415 Phosphorylation

Research Backgrounds

Function:

Histone methyltransferase. Preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3 forming H3K2me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.

Subcellular Location:

Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.

Subunit Structure:

Interacts with BRD4.

Family&Domains:

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.

Research Fields

· Metabolism > Amino acid metabolism > Lysine degradation.

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