NK1R Antibody | Affinity Biosciences

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Cites

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Western blot analysis of MCF7 cell lysates, using TACR1 Antibody.

Figure 4: Effects of EA treatment on the SP/NK1 receptor binding and NF-κB expression in rats after colonic anastomosis.

Product:	NK1R Antibody
Catalog:	DF7405
Description:	Rabbit polyclonal antibody to NK1R
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	46kDa; 46kD(Calculated).
Uniprot:	P25103
RRID:	AB_2839343

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

TACR1 Antibody detects endogenous levels of total TACR1.

RRID:

AB_2839343
Cite Format: Affinity Biosciences Cat# DF7405, RRID:AB_2839343.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Neurokinin 1 receptor; NK 1 receptor; NK 1R; NK-1 receptor; NK-1R; NK1 receptor; NK1R; NK1R_HUMAN; NKIR; SPR; Substance P receptor; Substance-P receptor; TAC 1R; TAC1R; Tachykinin 1 receptor (substance P receptor neurokinin 1 receptor); Tachykinin receptor 1 (substance P receptor neurokinin 1 receptor); Tachykinin receptor 1; TACR1;

Immunogens

Immunogen:

Uniprot:

P25103(NK1R_HUMAN) >>Visit HPA database.

Gene(ID):

TACR1(6869)

Description:

This gene belongs to a gene family of tachykinin receptors. These tachykinin receptors are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin substance P, also referred to as neurokinin 1. The encoded protein is also involved in the mediation of phosphatidylinositol metabolism of substance P.

Sequence:

P25103 NK1R_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDNVLPVDSDLSPNISTNTSEPNQFVQPAWQIVLWAAAYTVIVVTSVVGNVVVMWIILAHKRMRTVTNYFLVNLAFAEASMAAFNTVVNFTYAVHNEWYYGLFYCKFHNFFPIAAVFASIYSMTAVAFDRYMAIIHPLQPRLSATATKVVICVIWVLALLLAFPQGYYSTTETMPSRVVCMIEWPEHPNKIYEKVYHICVTVLIYFLPLLVIGYAYTVVGITLWASEIPGDSSDRYHEQVSAKRKVVKMMIVVVCTFAICWLPFHIFFLLPYINPDLYLKKFIQQVYLAIMWLAMSSTMYNPIIYCCLNDRFRLGFKHAFRCCPFISAGDYEGLEMKSTRYLQTQGSVYKVSRLETTISTVVGAHEEEPEDGPKATPSSLDLTSNCSSRSDSKTMTESFSFSSNVLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P25103 As Substrate

P25103

Site	PTM Type	Source
Y341	Phosphorylation	Uniprot
S347	Phosphorylation	Uniprot
S407	Phosphorylation	Uniprot

Research Backgrounds

P25103_NK1R_HUMAN

Function:

This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Subunit Structure:

Interacts with ARRB1.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Viral > Measles.

References

1). Substance P Mediates Estrogen Modulation Proinflammatory Cytokines Release in Intervertebral Disc. INFLAMMATION (PubMed: 32965648) [IF=5.1]

Application: IHC Species: mouse Sample: NP cells

Fig. 1.| Effects of ERT on SP and its receptor NK1R in NP cells. After OVX, the staining intensity of NK1R (a–c) and SP (d–f) in NP cells significantly increased compared with the sham group. While OVX+E2 could reverse these changes. Bar represents 50 μm.

2). Effects of Electroacupuncture on Gastrointestinal Motility Function, Pain, and Inflammation via Transient Receptor Potential Vanilloid 1 in a Rat Model after Colonic Anastomoses.

Application: ELISA Species: Rat Sample: colon tissues

Figure 4: Effects of EA treatment on the SP/NK1 receptor binding and NF-κB expression in rats after colonic anastomosis. (a) SP levels in the colon tissues were calculated using ELISA kits. (b) The expression of NK1 receptor mRNA in colon tissues of rats in each group was detected by quantitative real-time PCR. (c) Immunohistochemical staining for NK1 receptor in intestinal tissues (×400, scale bar = 50 μm). (d) The mean density of NK1 receptor-positive staining in colon tissues. (e) NF-κB (p65) protein expression in colon tissues of rats in each group was detected by western blot. (n = 8; ∗P < 0:05 and∗∗P < 0:01 versus control; #P < 0:05 versus model; one-way ANOVA followed by Tukey’s post hoc test).

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NK1R Antibody - #DF7405