ATIC Antibody - #DF7399

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Product: ATIC Antibody
Catalog: DF7399
Description: Rabbit polyclonal antibody to ATIC
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 64kDa; 65kD(Calculated).
Uniprot: P31939
RRID: AB_2839337

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ATIC Antibody detects endogenous levels of total ATIC.
RRID:
AB_2839337
Cite Format: Affinity Biosciences Cat# DF7399, RRID:AB_2839337.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AICAR formyltransferase/IMP cyclohydrolase; 5 aminoimidazole 4 carboxamide 1 beta D ribonucleotide transformylase/inosinicase; 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase; 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase; 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase; AICAR; AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme; AICAR transformylase; AICARFT; AICARFT/IMPCHASE; ATIC; Bifunctional purine biosynthesis protein PURH; epididymis secretory sperm binding protein Li 70p; FLJ93545; HEL-S-70p; IMP cyclohydrolase; IMP synthase; IMP synthetase; IMPCHASE; Inosinicase; OK/SW-cl.86; Phosphoribosylaminoimidazolecarboxamide formyltransferase; Phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase; PUR9_HUMAN; PURH;

Immunogens

Immunogen:
Uniprot:

P31939(PUR9_HUMAN) >>Visit HPA database.

Gene(ID):
ATIC(471)
Description:
This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria.
Sequence:
MAPGQLALFSVSDKTGLVEFARNLTALGLNLVASGGTAKALRDAGLAVRDVSELTGFPEMLGGRVKTLHPAVHAGILARNIPEDNADMARLDFNLIRVVACNLYPFVKTVASPGVTVEEAVEQIDIGGVTLLRAAAKNHARVTVVCEPEDYVVVSTEMQSSESKDTSLETRRQLALKAFTHTAQYDEAISDYFRKQYSKGVSQMPLRYGMNPHQTPAQLYTLQPKLPITVLNGAPGFINLCDALNAWQLVKELKEALGIPAAASFKHVSPAGAAVGIPLSEDEAKVCMVYDLYKTLTPISAAYARARGADRMSSFGDFVALSDVCDVPTAKIISREVSDGIIAPGYEEEALTILSKKKNGNYCVLQMDQSYKPDENEVRTLFGLHLSQKRNNGVVDKSLFSNVVTKNKDLPESALRDLIVATIAVKYTQSNSVCYAKNGQVIGIGAGQQSRIHCTRLAGDKANYWWLRHHPQVLSMKFKTGVKRAEISNAIDQYVTGTIGEDEDLIKWKALFEEVPELLTEAEKKEWVEKLTEVSISSDAFFPFRDNVDRAKRSGVAYIAAPSGSAADKVVIEACDELGIILAHTNLRLFHH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
86
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31939 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K14 Ubiquitination
T25 Phosphorylation
S34 Phosphorylation
T37 Phosphorylation
K39 Ubiquitination
R42 Methylation
S52 Phosphorylation
K66 Ubiquitination
R79 Methylation
Y104 Phosphorylation Q9UM73 (ALK)
K108 Ubiquitination
S112 Phosphorylation
K137 Ubiquitination
T166 Phosphorylation
K177 Ubiquitination
T180 Phosphorylation
T182 Phosphorylation
S190 Phosphorylation
Y192 Phosphorylation
K199 Acetylation
K199 Ubiquitination
Y208 Phosphorylation
Y220 Phosphorylation
K254 Ubiquitination
S264 Phosphorylation
K266 Acetylation
K266 Ubiquitination
S269 Phosphorylation
S280 Phosphorylation
K285 Ubiquitination
Y290 Phosphorylation
Y293 Phosphorylation
K294 Ubiquitination
T295 Phosphorylation
T297 Phosphorylation
S300 Phosphorylation
Y303 Phosphorylation
R305 Methylation
K331 Ubiquitination
S338 Phosphorylation
S355 Phosphorylation
K356 Acetylation
K356 Ubiquitination
K357 Ubiquitination
K372 Ubiquitination
S387 Phosphorylation
K389 Ubiquitination
K397 Ubiquitination
S398 Phosphorylation
S401 Phosphorylation
T405 Phosphorylation
K406 Ubiquitination
K408 Ubiquitination
S413 Phosphorylation
T422 Phosphorylation
C434 S-Nitrosylation
K437 Ubiquitination
K461 Ubiquitination
Y464 Phosphorylation
K477 Ubiquitination
K507 Sumoylation
K507 Ubiquitination
K509 Sumoylation
K509 Ubiquitination
T520 Phosphorylation
K524 Ubiquitination
S537 Phosphorylation

Research Backgrounds

Function:

Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis.

Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization.

Subunit Structure:

Homodimer. Associates with internalized INSR complexes on Golgi/endosomal membranes. Interacts with INSR; ATIC together with PRKAA2/AMPK2 and HACD3/PTPLAD1 is proposed to be part of a signaling network regulating INSR autophosphorylation and endocytosis.

Family&Domains:

The IMP cyclohydrolase activity resides in the N-terminal region.

Belongs to the PurH family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.

· Metabolism > Global and overview maps > Metabolic pathways.

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