Asparagine synthetase Antibody - #DF7398

(2)  
Product: Asparagine synthetase Antibody
Catalog: DF7398
Description: Rabbit polyclonal antibody to Asparagine synthetase
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 64kDa; 64kD(Calculated).
Uniprot: P08243
RRID: AB_2839336

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Asparagine synthetase Antibody detects endogenous levels of total Asparagine synthetase.
RRID:
AB_2839336
Cite Format: Affinity Biosciences Cat# DF7398, RRID:AB_2839336.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

asnS; ASNS_HUMAN; ASNSD; Asparagine synthetase [glutamine-hydrolyzing]; Asparagine synthetase; Cell cycle control protein TS11; Glutamine dependent asparagine synthetase 3; Glutamine dependent asparagine synthetase; Glutamine hydrolyzing; Glutamine-dependent asparagine synthetase; OTTHUMP00000024510; OTTHUMP00000204938; OTTHUMP00000204939; OTTHUMP00000204940; OTTHUMP00000204941; OTTHUMP00000204942; TS11; TS11 cell cycle control protein;

Immunogens

Immunogen:
Uniprot:

P08243(ASNS_HUMAN) >>Visit HPA database.

Gene(ID):
ASNS(440)
Description:
The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene.
Sequence:
MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVDPLFGMQPIRVKKYPYLWLCYNGEIYNHKKMQQHFEFEYQTKVDGEIILHLYDKGGIEQTICMLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVTLKHSATPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHALYDNVEKLFPGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESERLLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRETFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMANAAQKFPFNTPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSARTLTHYKSAVKA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Horse
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08243 As Substrate

Site PTM Type Enzyme
K83 Ubiquitination
Y92 Phosphorylation
K132 Ubiquitination
K147 Methylation
K147 Ubiquitination
K162 Ubiquitination
K168 Ubiquitination
K176 Sumoylation
K176 Ubiquitination
Y185 Phosphorylation
K191 Acetylation
K191 Ubiquitination
K195 Ubiquitination
K203 Ubiquitination
K221 Ubiquitination
K232 Ubiquitination
K244 Ubiquitination
K245 Ubiquitination
S258 Phosphorylation
K272 Ubiquitination
K275 Ubiquitination
K350 Ubiquitination
K379 Ubiquitination
K385 Acetylation
K385 Ubiquitination
R407 Methylation
R417 Methylation
K440 Ubiquitination
K445 Ubiquitination
R449 Methylation
K460 Ubiquitination
K467 Methylation
K467 Ubiquitination
S471 Phosphorylation
S476 Phosphorylation
K478 Ubiquitination
K483 Ubiquitination
K504 Ubiquitination
K511 Ubiquitination
K513 Ubiquitination
R519 Methylation
R524 Methylation
K540 Ubiquitination
T545 Phosphorylation
S548 Phosphorylation
T551 Phosphorylation
Y555 Phosphorylation
K556 Methylation
K556 Ubiquitination
S557 Phosphorylation

Research Backgrounds

Research Fields

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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