RPLP0 Antibody - #DF7397

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Product: RPLP0 Antibody
Catalog: DF7397
Description: Rabbit polyclonal antibody to RPLP0
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 34kDa; 34kD(Calculated).
Uniprot: P05388
RRID: AB_2839335

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
RPLP0 Antibody detects endogenous levels of total RPLP0.
RRID:
AB_2839335
Cite Format: Affinity Biosciences Cat# DF7397, RRID:AB_2839335.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

36B4; 60S acidic ribosomal protein P0; 60S ribosomal protein L10E; Acidic ribosomal phosphoprotein P0; Arbp; L10E; LP0; MGC107165; MGC107166; MGC111226; MGC88175; OTTMUSP00000015585; P0; PRLP0; Ribosomal phosphoprotein, acidic, P0; Ribosomal protein P0; Ribosomal protein, large, P0; RLA0_HUMAN; rplP0; RPP0;

Immunogens

Immunogen:
Uniprot:

P05388(RLA0_HUMAN) >>Visit HPA database.

Gene(ID):
RPLP0(6175)
Description:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Sequence:
MPREDRATWKSNYFLKIIQLLDDYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENNPALEKLLPHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCEVTVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLVIQQVFDNGSIYNPEVLDITEETLHSRFLEGVRNVASVCLQIGYPTVASVPHSIINGYKRVLALSVETDYTFPLAEKVKAFLADPSAFVAAAPVAAATTAAPAAAAAPAKVEAKEESEESDEDMGFGLFD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
83
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05388 As Substrate

Site PTM Type Enzyme
R6 Methylation
T8 Phosphorylation
K10 Acetylation
K10 Methylation
K10 Ubiquitination
K16 Methylation
K16 Ubiquitination
Y24 Phosphorylation
K26 Ubiquitination
C27 S-Nitrosylation
S37 Phosphorylation
K38 Ubiquitination
K50 Ubiquitination
K57 Ubiquitination
T59 Phosphorylation
R66 Methylation
K77 Acetylation
K77 Ubiquitination
K92 Ubiquitination
K106 Acetylation
K106 Ubiquitination
R112 Methylation
C119 S-Nitrosylation
T122 Phosphorylation
T128 Phosphorylation
K134 Ubiquitination
T135 Phosphorylation
S136 Phosphorylation
K146 Ubiquitination
S148 Phosphorylation
T151 Phosphorylation
S156 Phosphorylation
K162 Ubiquitination
K166 Ubiquitination
K246 Acetylation
K246 Ubiquitination
S252 Phosphorylation
Y257 Phosphorylation
K264 Ubiquitination
K266 Ubiquitination
S273 Phosphorylation
T285 Phosphorylation
T286 Phosphorylation
K297 Sumoylation
K297 Ubiquitination
S304 Phosphorylation
S307 Phosphorylation

Research Backgrounds

Function:

Ribosomal protein P0 is the functional equivalent of E.coli protein L10.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:19188445, PubMed:17289661).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

P0 forms a pentameric complex by interaction with dimers of P1 and P2. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with APEX1. Interacts with FMR1 isoform 6.

Family&Domains:

Belongs to the universal ribosomal protein uL10 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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