MCM5 Antibody - #DF7396

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Product: MCM5 Antibody
Catalog: DF7396
Description: Rabbit polyclonal antibody to MCM5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 82kDa; 82kD(Calculated).
Uniprot: P33992
RRID: AB_2839334

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MCM5 Antibody detects endogenous levels of total MCM5.
RRID:
AB_2839334
Cite Format: Affinity Biosciences Cat# DF7396, RRID:AB_2839334.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDC 46; CDC46; CDC46 homolog; Cell division cycle 46; DNA replication licensing factor; DNA replication licensing factor MCM 5; DNA replication licensing factor MCM5; MCM 5; MCM5; MCM5_HUMAN; MGC5315; Minichromosome maintenance complex component 5; Minichromosome maintenance deficient (S. cerevisiae) 5; Minichromosome maintenance deficient 5; Minichromosome maintenance deficient protein 5; P1 CDC46; P1-CDC46;

Immunogens

Immunogen:
Uniprot:

P33992(MCM5_HUMAN) >>Visit HPA database.

Gene(ID):
MCM5(4174)
Description:
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Sequence:
MSGFDDPGIFYSDSFGGDAQADEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELKRHYNLGEYWIEVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEVLQDIQVMLKSDASPSSIRSLKSDMMSHLVKIPGIIIAASAVRAKATRISIQCRSCRNTLTNIAMRPGLEGYALPRKCNTDQAGRPKCPLDPYFIMPDKCKCVDFQTLKLQELPDAVPHGEMPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFGLTTSRGRDRVGVGIRSSYIRVLGIQVDTDGSGRSFAGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGTDMKKAIACLLFGGSRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
86
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P33992 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S12 Phosphorylation
S14 Phosphorylation
K36 Ubiquitination
K54 Ubiquitination
K129 Ubiquitination
S130 Phosphorylation
S133 Phosphorylation
K141 Ubiquitination
K150 Ubiquitination
S159 Phosphorylation
K206 Ubiquitination
Y212 Phosphorylation
K218 Ubiquitination
K220 Acetylation
K220 Ubiquitination
K228 Ubiquitination
Y248 Phosphorylation
R251 Methylation
K256 Ubiquitination
Y269 Phosphorylation
K273 Ubiquitination
S279 Phosphorylation
R280 Methylation
T303 Phosphorylation
S315 Phosphorylation
Y332 Phosphorylation
S336 Phosphorylation
K337 Ubiquitination
S338 Phosphorylation
S342 Phosphorylation
K350 Ubiquitination
K351 Ubiquitination
S361 Phosphorylation
K363 Ubiquitination
T385 Phosphorylation
K387 Acetylation
K387 Ubiquitination
K392 Acetylation
K392 Ubiquitination
K396 Acetylation
K396 Ubiquitination
Y403 Phosphorylation
K407 Ubiquitination
S423 Phosphorylation
S424 Phosphorylation
K471 Ubiquitination
C482 S-Nitrosylation
K499 Ubiquitination
R528 Methylation
K561 Ubiquitination
K581 Acetylation
K581 Ubiquitination
K583 Ubiquitination
S604 Phosphorylation
S605 Phosphorylation
K625 Ubiquitination
K627 Ubiquitination
T633 Phosphorylation O14757 (CHEK1)
S693 Phosphorylation
K696 Acetylation
K696 Ubiquitination
K702 Ubiquitination

Research Backgrounds

Function:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.

Subcellular Location:

Nucleus. Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with ANKRD17.

Family&Domains:

Belongs to the MCM family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Genetic Information Processing > Replication and repair > DNA replication.

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