PSMF1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using PSMF1 Antibody.

Product:	PSMF1 Antibody
Catalog:	DF7394
Description:	Rabbit polyclonal antibody to PSMF1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	29kDa; 30kD(Calculated).
Uniprot:	Q92530
RRID:	AB_2839332

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

PSMF1 Antibody detects endogenous levels of total PSMF1.

RRID:

AB_2839332
Cite Format: Affinity Biosciences Cat# DF7394, RRID:AB_2839332.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

hPI31; PI31; Proteasome (prosome macropain) inhibitor subunit 1; Proteasome inhibitor hP131 subunit; Proteasome inhibitor PI31 subunit; PSMB2; Psmf1; PSMF1_HUMAN; RP23 402M7.5; RP4 545L17.1;

Immunogens

Immunogen:

Uniprot:

Q92530(PSMF1_HUMAN) >>Visit HPA database.

Gene(ID):

PSMF1(9491)

Description:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.

Sequence:

Q92530 PSMF1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGLEVLFASAAPAITCRQDALVCFLHWEVVTHGYFGLGVGDQPGPNDKKSELLPAGWNNNKDLYVLRYEYKDGSRKLLVKAITVESSMILNVLEYGSQQVADLTLNLDDYIDAEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPPATAREVDPLRIPPHHPHTSRQPPWCDPLGPFVVGGEDLDPFGPRRGGMIVDPLRSGFPRALIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPPGPNPDHLPPPGYDDMYL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92530 As Substrate

Q92530

Site	PTM Type	Source
A2	Acetylation	Uniprot
K50	Ubiquitination	Uniprot
K125	Ubiquitination	Uniprot
S136	Phosphorylation	Uniprot
T140	Phosphorylation	Uniprot
K148	Ubiquitination	Uniprot
S152	Phosphorylation	Uniprot
S153	Phosphorylation	Uniprot
R205	Methylation	Uniprot
R214	Methylation	Uniprot
R219	Methylation	Uniprot
R231	Methylation	Uniprot
T251	Phosphorylation	Uniprot
S252	Phosphorylation	Uniprot
Y266	Phosphorylation	Uniprot
Y270	Phosphorylation	Uniprot

Research Backgrounds

Q92530_PSMF1_HUMAN

Function:

Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.

Subcellular Location:

Cytoplasm. Endoplasmic reticulum.

Subunit Structure:

Monomer and homodimer. Interacts with FBXO7. Interacts with the 20S proteasome.

Family&Domains:

Belongs to the proteasome inhibitor PI31 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

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For Research Use Only.
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PSMF1 Antibody - #DF7394