HAX1 Antibody - #DF7391

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Product: HAX1 Antibody
Catalog: DF7391
Description: Rabbit polyclonal antibody to HAX1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 31kDa; 32kD(Calculated).
Uniprot: O00165
RRID: AB_2839329

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
HAX1 Antibody detects endogenous levels of total HAX1.
RRID:
AB_2839329
Cite Format: Affinity Biosciences Cat# DF7391, RRID:AB_2839329.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ17042; FLJ18492; FLJ93803; HAX 1; HAX-1; HAX1; HAX1_HUMAN; Hax1a; HCLS1 and PKD2 associated protein; HCLS1 associated protein; HCLS1 associated protein X 1; HCLS1-associated protein X-1; HCLSBP1; HS 1 associated protein X 1; HS 1 binding protein; HS1 associating protein X 1; HS1 binding protein 1; HS1 binding protein; HS1-associating protein X-1; HS1-binding protein 1; HS1BP1; HSP1BP-1; OTTHUMP00000034190; SCN3;

Immunogens

Immunogen:
Uniprot:

O00165(HAX1_HUMAN) >>Visit HPA database.

Gene(ID):
HAX1(10456)
Expression:
O00165 HAX1_HUMAN:

Ubiquitous. Up-regulated in oral cancers.

Description:
The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene.
Sequence:
MSLFDLFRGFFGFPGPRSHRDPFFGGMTRDEDDDEEEEEEGGSWGRGNPRFHSPQHPPEEFGFGFSFSPGGGIRFHDNFGFDDLVRDFNSIFSDMGAWTLPSHPPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFGGVLESDARSESPQPAPDWGSQRPFHRFDDVWPMDPHPRTREDNDLDSQVSQEGLGPVLQPQPKSYFKSISVTKITKPDGIVEERRTVVDSEGRTETTVTRHEADSSPRGDPESPRPPALDDAFSILDLFLGRWFRSR

PTMs - O00165 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S53 Phosphorylation
S66 Phosphorylation
S68 Phosphorylation
T124 Phosphorylation
K131 Ubiquitination
R139 Methylation
S153 Phosphorylation
S162 Phosphorylation
T181 Phosphorylation
S189 Phosphorylation
S192 Phosphorylation
K205 Ubiquitination
K209 Ubiquitination
S210 Phosphorylation
S212 Phosphorylation
T214 Phosphorylation
K215 Ubiquitination
K218 Ubiquitination
S255 Phosphorylation
S266 Phosphorylation

Research Backgrounds

Function:

Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex. Slows down the rate of inactivation of KCNC3 channels. Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.

PTMs:

Proteolytically cleaved by caspase-3 during apoptosis.

Subcellular Location:

Mitochondrion. Endoplasmic reticulum. Nucleus membrane. Cytoplasmic vesicle. Cytoplasm>Cell cortex. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Sarcoplasmic reticulum. Cytoplasm>P-body.

Cytoplasm. Nucleus.
Note: Predominantly cytoplasmic. Also detected in the nucleus when nuclear export is inhibited, and in response to cellular stress caused by arsenite (in vitro).

Cytoplasm. Nucleus.
Note: Predominantly cytoplasmic. Also detected in the nucleus when nuclear export is inhibited (in vitro).

Cytoplasm. Nucleus.
Note: Shuttles between nucleus and cytoplasm.

Cytoplasm.
Note: Predominantly cytoplasmic.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Up-regulated in oral cancers.

Subunit Structure:

Interacts with ABCB1, ABCB4 and ABCB11 (By similarity). Directly associates with HCLS1/HS1, through binding to its N-terminal region. Interacts with CTTN (By similarity). Interacts with PKD2. Interacts with GNA13. Interacts with CASP9. Interacts with ITGB6. Interacts with PLN and ATP2A2; these interactions are inhibited by calcium. Interacts with GRB7. Interacts (via C-terminus) with XIAP/BIRC4 (via BIR 2 domain and BIR 3 domain) and this interaction blocks ubiquitination of XIAP/BIRC4. Interacts with TPC2. Interacts with KCNC3. Interacts with XPO1.

Family&Domains:

Belongs to the HAX1 family.

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