HNRNPF Antibody - #DF7383

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Product: HNRNPF Antibody
Catalog: DF7383
Description: Rabbit polyclonal antibody to HNRNPF
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 45kDa; 46kD(Calculated).
Uniprot: P52597
RRID: AB_2839321

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(88%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
HNRNPF Antibody detects endogenous levels of total HNRNPF.
RRID:
AB_2839321
Cite Format: Affinity Biosciences Cat# DF7383, RRID:AB_2839321.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Heterogeneous nuclear ribonucleoprotein F; hnRNP F; HnRNP F protein; Hnrnpf; HNRPF; HNRPF_HUMAN; mcs94 1; MGC110997; N-terminally processed; Nucleolin like protein; Nucleolin like protein mcs94 1; Nucleolin-like protein mcs94-1; OK/SW-cl.23; OTTHUMP00000019482; OTTHUMP00000043413; OTTHUMP00000043414; Ribonucleoprotein F;

Immunogens

Immunogen:
Uniprot:

P52597(HNRPF_HUMAN) >>Visit HPA database.

Gene(ID):
HNRNPF(3185)
Expression:
P52597 HNRPF_HUMAN:

Expressed ubiquitously.

Description:
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Sequence:
MMLGPEGGEGFVVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMALKKDRESMGHRYIEVFKSHRTEMDWVLKHSGPNSADSANDGFVRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEEVRSYSDPPLKFMSVQRPGPYDRPGTARRYIGIVKQAGLERMRPGAYSTGYGGYEEYSGLSDGYGFTTDLFGRDLSYCLSGMYDHRYGDSEFTVQSTTGHCVHMRGLPYKATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEEAVAAMSKDRANMQHRYIELFLNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNSMGGYD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
100
Horse
88
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52597 As Substrate

Site PTM Type Enzyme
M1 Acetylation
M2 Acetylation
K14 Ubiquitination
S23 Phosphorylation
S63 Phosphorylation
K68 Sumoylation
K72 Sumoylation
S77 Phosphorylation
Y82 Phosphorylation
K87 Acetylation
K87 Methylation
K87 Ubiquitination
R90 Methylation
K98 Acetylation
K98 Ubiquitination
S104 Phosphorylation
S107 Phosphorylation
R114 Methylation
T142 Phosphorylation
K150 Sumoylation
T152 Phosphorylation
S161 Phosphorylation
K167 Ubiquitination
K171 Ubiquitination
Y180 Phosphorylation
K185 Sumoylation
K185 Ubiquitination
S186 Phosphorylation
S187 Phosphorylation
S193 Phosphorylation
S195 Phosphorylation
K200 Sumoylation
K200 Ubiquitination
S203 Phosphorylation
Y210 Phosphorylation
R212 Methylation
T215 Phosphorylation
R217 Methylation
R218 Methylation
Y219 Phosphorylation
K224 Acetylation
K224 Methylation
K224 Sumoylation
K224 Ubiquitination
R232 Methylation
Y236 Phosphorylation
Y240 Phosphorylation
Y243 Phosphorylation
Y246 Phosphorylation
S247 Phosphorylation
Y253 Phosphorylation
S265 Phosphorylation
Y266 Phosphorylation
C267 S-Nitrosylation
S269 Phosphorylation
Y272 Phosphorylation
Y276 Phosphorylation
S285 Phosphorylation
T286 Phosphorylation
K299 Ubiquitination
T301 Phosphorylation
Y306 Phosphorylation
S310 Phosphorylation
K347 Ubiquitination
Y401 Phosphorylation

Research Backgrounds

Function:

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.

PTMs:

Sumoylated.

Subcellular Location:

Nucleus>Nucleoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed ubiquitously.

Subunit Structure:

Identified in the spliceosome C complex. Interacts with AGO1, AGO2, TBP and TXNL4/DIM1.

Family&Domains:

The N-terminal RRM domains are responsible for recognizing the G-tract of BCL-X RNA.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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