CTSA Antibody - #DF7381

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Product: CTSA Antibody
Catalog: DF7381
Description: Rabbit polyclonal antibody to CTSA
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 54kDa; 54kD(Calculated).
Uniprot: P10619
RRID: AB_2839319

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CTSA Antibody detects endogenous levels of total CTSA.
RRID:
AB_2839319
Cite Format: Affinity Biosciences Cat# DF7381, RRID:AB_2839319.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

beta galactosidase 2; BETA GALACTOSIDASE PROTECTIVE PROTEIN; beta-galactosidase 2; beta-galactosidase protective protein; betagalactosidase 2; Carboxypeptidase C; Carboxypeptidase L; carboxypeptidase Y-like kininase; Cathepsin A; Ctsa; deamidase; EC 3.4.16.5; Glactosialidosis; GLB2; Goldberg Syndrome; GSL; lysosomal carboxypeptidase A; Lysosomal protective protein 20 kDa chain; Lysosomal protective protein; Lysosomal protective protein deficiency; NEURAMINIDASE BETA GALACTOSIDASE EXPRESSION; NGBE; Neuraminidase deficiency with beta-galactosidase deficiency; NGBE; OTTHUMP00000031778; OTTHUMP00000031781; PPCA; PPCA deficiency; PPGB; PPGB_HUMAN; Protective protein cathepsin A; Protective protein for beta galactosidase; Protective protein for beta-galactosidase; Protective protein/cathepsin A deficiency; urinary kininase;

Immunogens

Immunogen:
Uniprot:

P10619(PPGB_HUMAN) >>Visit HPA database.

Gene(ID):
CTSA(5476)
Description:
This gene encodes a glycoprotein which associates with lysosomal enzymes beta-galactosidase and neuraminidase to form a complex of high molecular weight multimers. The formation of this complex provides a protective role for stability and activity. Deficiencies in this gene are linked to multiple forms of galactosialidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Sequence:
MIRAAPPPLFLLLLLLLLLVSWASRGEAAPDQDEIQRLPGLAKQPSFRQYSGYLKGSGSKHLHYWFVESQKDPENSPVVLWLNGGPGCSSLDGLLTEHGPFLVQPDGVTLEYNPYSWNLIANVLYLESPAGVGFSYSDDKFYATNDTEVAQSNFEALQDFFRLFPEYKNNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLSSYEQNDNSLVYFAYYHGLLGNRLWSSLQTHCCSQNKCNFYDNKDLECVTNLQEVARIVGNSGLNIYNLYAPCAGGVPSHFRYEKDTVVVQDLGNIFTRLPLKRMWHQALLRSGDKVRMDPPCTNTTAASTYLNNPYVRKALNIPEQLPQWDMCNFLVNLQYRRLYRSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVKYGDSGEQIAGFVKEFSHIAFLTIKGAGHMVPTDKPLAAFTMFSRFLNKQPY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
70
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10619 As Substrate

Site PTM Type Enzyme
K55 Methylation
N145 N-Glycosylation
T295 Phosphorylation
N333 N-Glycosylation
K429 Ubiquitination
Y430 Phosphorylation
T469 Phosphorylation
S472 Phosphorylation

Research Backgrounds

Function:

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.

Subcellular Location:

Lysosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked.

Family&Domains:

Belongs to the peptidase S10 family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Organismal Systems > Endocrine system > Renin-angiotensin system.   (View pathway)

Restrictive clause

 

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For Research Use Only.
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