Dnmt1 Antibody | Affinity Biosciences

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Product:	Dnmt1 Antibody
Catalog:	DF7376
Description:	Rabbit polyclonal antibody to Dnmt1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep
Mol.Wt.:	183kDa; 183kD(Calculated).
Uniprot:	P26358
RRID:	AB_2839314

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(91%), Horse(100%), Sheep(91%)

Clonality:

Polyclonal

Specificity:

Dnmt1 Antibody detects endogenous levels of total Dnmt1.

RRID:

AB_2839314
Cite Format: Affinity Biosciences Cat# DF7376, RRID:AB_2839314.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADCADN; AIM; CXXC finger protein 9; CXXC-type zinc finger protein 9; CXXC9; DNA (cytosine 5 ) methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase 1; DNA methyltransferase HsaI; DNA methyltransferase M.HsaI.; DNA MTase; DNA MTase HsaI; DNMT 1; DNMT; Dnmt1; DNMT1_HUMAN; Dnmt1o; FLJ16293; HSN1E; M.HsaI; MCMT; Met1; MGC104992; mMmul; MommeD2;

Immunogens

Immunogen:

Uniprot:

P26358(DNMT1_HUMAN) >>Visit HPA database.

Gene(ID):

DNMT1(1786)

Expression:

P26358 DNMT1_HUMAN:

Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.

Description:

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene.

Sequence:

P26358 DNMT1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

Sheep

Chicken

Pig

Dog

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P26358 As Substrate

P26358

Site	PTM Type	Enzyme	Source
K45	Ubiquitination		Uniprot
K58	Ubiquitination		Uniprot
K67	Ubiquitination		Uniprot
K70	Methylation		Uniprot
K70	Ubiquitination		Uniprot
K81	Ubiquitination		Uniprot
K83	Ubiquitination		Uniprot
S84	Phosphorylation		Uniprot
K88	Ubiquitination		Uniprot
S127	Phosphorylation		Uniprot
S133	Phosphorylation		Uniprot
K134	Ubiquitination		Uniprot
T137	Phosphorylation		Uniprot
S141	Phosphorylation		Uniprot
K142	Methylation		Uniprot
S143	Phosphorylation	P31749 (AKT1)	Uniprot
K148	Sumoylation		Uniprot
S152	Phosphorylation		Uniprot
S154	Phosphorylation	P06493 (CDK1) , Q00535 (CDK5) , P24941 (CDK2)	Uniprot
K160	Acetylation		Uniprot
T165	Phosphorylation		Uniprot
T166	Phosphorylation		Uniprot
T168	Phosphorylation		Uniprot
K173	Acetylation		Uniprot
K173	Ubiquitination		Uniprot
K188	Acetylation		Uniprot
S189	Phosphorylation		Uniprot
S192	Phosphorylation		Uniprot
K194	Sumoylation		Uniprot
T208	Phosphorylation		Uniprot
S209	Phosphorylation		Uniprot
S227	Phosphorylation		Uniprot
S247	Phosphorylation		Uniprot
K259	Acetylation		Uniprot
K259	Sumoylation		Uniprot
S288	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
T328	Phosphorylation		Uniprot
K335	Acetylation		Uniprot
K336	Ubiquitination		Uniprot
S346	Phosphorylation		Uniprot
Y359	Phosphorylation		Uniprot
K366	Acetylation		Uniprot
K385	Ubiquitination		Uniprot
S394	Phosphorylation		Uniprot
S398	Phosphorylation		Uniprot
Y399	Phosphorylation		Uniprot
S549	Phosphorylation		Uniprot
K586	Ubiquitination		Uniprot
T591	Phosphorylation		Uniprot
K638	Ubiquitination		Uniprot
K644	Ubiquitination		Uniprot
K666	Ubiquitination		Uniprot
K668	Ubiquitination		Uniprot
K675	Sumoylation		Uniprot
K675	Ubiquitination		Uniprot
S714	Phosphorylation	P42345 (MTOR)	Uniprot
K716	Ubiquitination		Uniprot
S732	Phosphorylation		Uniprot
K739	Sumoylation		Uniprot
K749	Acetylation		Uniprot
S878	Phosphorylation		Uniprot
K881	Ubiquitination		Uniprot
T882	Phosphorylation		Uniprot
K889	Ubiquitination		Uniprot
K891	Acetylation		Uniprot
S919	Phosphorylation		Uniprot
K928	Ubiquitination		Uniprot
S954	Phosphorylation		Uniprot
K957	Acetylation		Uniprot
K957	Sumoylation		Uniprot
K957	Ubiquitination		Uniprot
K961	Acetylation		Uniprot
K961	Ubiquitination		Uniprot
Y969	Phosphorylation		Uniprot
K975	Acetylation		Uniprot
K975	Ubiquitination		Uniprot
S977	Phosphorylation		Uniprot
K981	Methylation		Uniprot
K981	Ubiquitination		Uniprot
S983	Phosphorylation		Uniprot
K997	Ubiquitination		Uniprot
K1020	Ubiquitination		Uniprot
K1054	Acetylation		Uniprot
K1092	Ubiquitination		Uniprot
K1094	Ubiquitination		Uniprot
S1105	Phosphorylation		Uniprot
K1111	Acetylation		Uniprot
K1113	Acetylation		Uniprot
K1115	Acetylation		Uniprot
K1117	Acetylation		Uniprot
K1119	Acetylation		Uniprot
K1121	Acetylation		Uniprot
S1122	Phosphorylation		Uniprot
K1135	Sumoylation		Uniprot
K1135	Ubiquitination		Uniprot
K1196	Ubiquitination		Uniprot
T1204	Phosphorylation		Uniprot
K1214	Ubiquitination		Uniprot
Y1254	Phosphorylation		Uniprot
K1323	Ubiquitination		Uniprot
K1349	Acetylation		Uniprot
K1349	Ubiquitination		Uniprot
S1358	Phosphorylation		Uniprot
R1401	Methylation		Uniprot
Y1405	Phosphorylation		Uniprot
K1415	Acetylation		Uniprot
K1415	Ubiquitination		Uniprot
S1447	Phosphorylation		Uniprot
R1473	Methylation		Uniprot
K1483	Ubiquitination		Uniprot
S1524	Phosphorylation		Uniprot
T1525	Phosphorylation		Uniprot
T1526	Phosphorylation		Uniprot
K1535	Ubiquitination		Uniprot
K1572	Ubiquitination		Uniprot
K1586	Ubiquitination		Uniprot
K1599	Ubiquitination		Uniprot
S1605	Phosphorylation		Uniprot
K1609	Sumoylation		Uniprot

Research Backgrounds

P26358_DNMT1_HUMAN

Function:

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth.

PTMs:

Sumoylated; sumoylation increases activity.

Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity.

Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability.

Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation.

Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.

Subcellular Location:

Nucleus.

Tissue Specificity:

Subunit Structure:

Homodimer. Forms a stable complex with E2F1, BB1 and HDAC1. Forms a complex with DMAP1 and HDAC2, with direct interaction. Interacts with the PRC2/EED-EZH2 complex. Probably part of a corepressor complex containing ZNF304, TRIM28, SETDB1 and DNMT1. Interacts with UHRF1; promoting its recruitment to hemimethylated DNA. Interacts with USP7, promoting its deubiquitination. Interacts with PCNA. Interacts with MBD2 and MBD3. Interacts with DNMT3A and DNMT3B. Interacts with UBC9. Interacts with CSNK1D (By similarity). Interacts with HDAC1 (By similarity). Interacts with BAZ2A/TIP5 (By similarity). Interacts with SIRT7 (By similarity).

Family&Domains:

The N-terminal part is required for homodimerization and acts as a regulatory domain.

The CXXC-type zinc finger specifically binds to unmethylated CpG dinucleotides, positioning the autoinhibitory linker between the DNA and the active site, thus providing a mechanism to ensure that only hemimethylated CpG dinucleotides undergo methylation.

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Decreased ovarian function and autophagy gene methylation in aging rats. Journal of Ovarian Research (PubMed: 32014030) [IF=4.0]

Application: WB Species: rat Sample: ovaries

Fig. 6| Protein expression of Dnmt1, Dnmt2, Dnmt3A and Dnmt3B in the ovaries of rats.(a) Western blot results.

2). Cissus quadrangularis extract attenuates diabetic nephropathy by altering SIRT1/DNMT1 axis. JOURNAL OF PHARMACY AND PHARMACOLOGY (PubMed: 34128987) [IF=3.3]

3). Tacrolimus Maintains the Balance of Neutrophil Extracellular Traps by Inducing DNA Methylation of Neutrophils to Reduce Immune Rejection. Life (Basel, Switzerland) (PubMed: 38137854) [IF=3.2]

4). Determination of DNA Methyltransferase 1 in Cells Using a RG108-Fluorescein Conjugate to Monitor the Fluorescent Ratio with a Microplate Reader. ANALYTICAL LETTERS [IF=2.0]

5). The expressions of DNA methyltransferase 1 (DNMT1) and cyclin A1 (CCNA1) in cervical carcinogenesis. International Journal of Clinical and Experimental Pathology (PubMed: 31933719) [IF=1.4]

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Dnmt1 Antibody - #DF7376