RFC4 Antibody - #DF7367

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Product: RFC4 Antibody
Catalog: DF7367
Description: Rabbit polyclonal antibody to RFC4
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 39kDa; 40kD(Calculated).
Uniprot: P35249
RRID: AB_2839305

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
RFC4 Antibody detects endogenous levels of total RFC4.
RRID:
AB_2839305
Cite Format: Affinity Biosciences Cat# DF7367, RRID:AB_2839305.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A1 37; A1 37 kDa subunit; Activator 1 37; Activator 1 37 kDa subunit; Activator 1 subunit 4; Replication factor C 37; Replication factor C 37 kDa subunit; Replication factor C subunit 4; Replication factor C4; RF-C 37 kDa subunit; RFC 37; RFC37; RFC4 replication factor C (activator 1)4 37kDa; RfC4; RFC4_HUMAN;

Immunogens

Immunogen:
Uniprot:

P35249(RFC4_HUMAN) >>Visit HPA database.

Gene(ID):
RFC4(5984)
Description:
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
Sequence:
MQAFLKGTSISTKPPLTKDRGVAASAGSSGENKKAKPVPWVEKYRPKCVDEVAFQEEVVAVLKKSLEGADLPNLLFYGPPGTGKTSTILAAARELFGPELFRLRVLELNASDERGIQVVREKVKNFAQLTVSGSRSDGKPCPPFKIVILDEADSMTSAAQAALRRTMEKESKTTRFCLICNYVSRIIEPLTSRCSKFRFKPLSDKIQQQRLLDIAKKENVKISDEGIAYLVKVSEGDLRKAITFLQSATRLTGGKEITEKVITDIAGVIPAEKIDGVFAACQSGSFDKLEAVVKDLIDEGHAATQLVNQLHDVVVENNLSDKQKSIITEKLAEVDKCLADGADEHLQLISLCATVMQQLSQNC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
91
Sheep
77
Zebrafish
73
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35249 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K6 Acetylation
K6 Methylation
K6 Ubiquitination
S9 Phosphorylation
S11 Phosphorylation
T12 Phosphorylation
K13 Acetylation
K13 Ubiquitination
T17 Phosphorylation
K18 Ubiquitination
R20 Methylation
S25 Phosphorylation
S28 Phosphorylation
S29 Phosphorylation
K34 Ubiquitination
K36 Ubiquitination
K43 Ubiquitination
K47 Ubiquitination
K63 Ubiquitination
K64 Ubiquitination
K84 Ubiquitination
R114 Methylation
K124 Ubiquitination
K139 Ubiquitination
T166 Phosphorylation
T174 Phosphorylation
S184 Phosphorylation
T191 Phosphorylation
S192 Phosphorylation
K200 Ubiquitination
K205 Ubiquitination
K216 Acetylation
K216 Ubiquitination
K217 Acetylation
K221 Ubiquitination
K232 Ubiquitination
K240 Ubiquitination
K255 Ubiquitination
K260 Ubiquitination
S285 Phosphorylation
K288 Ubiquitination
K294 Ubiquitination
K322 Ubiquitination
K324 Ubiquitination
K330 Ubiquitination

Research Backgrounds

Function:

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA.

Family&Domains:

Belongs to the activator 1 small subunits family.

Research Fields

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Mismatch repair.

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