PRPF3 Antibody - #DF7364
| Product: | PRPF3 Antibody |
| Catalog: | DF7364 |
| Description: | Rabbit polyclonal antibody to PRPF3 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 78kDa; 78kD(Calculated). |
| Uniprot: | O43395 |
| RRID: | AB_2839302 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF7364, RRID:AB_2839302.
Fold/Unfold
hPrp3; HPRP3P; Pre mRNA splicing factor 3; Pre-mRNA-splicing factor 3; Precursor mRNA-processing factor 3, S. crevisiae, homolog of; PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae); Prp3p; PRPF3; PRPF3_HUMAN; RP18; SNRNP90; U4/U6 small nuclear ribonucleoprotein PRP3; U4/U6 snRNP 90 kDa protein;
Immunogens
Highly expressed in retina, liver, kidney and blood. Detected at lower levels in heart and brain.
- O43395 PRPF3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALSKRELDELKPWIEKTVKRVLGFSEPTVVTAALNCVGKGMDKKKAADHLKPFLDDSTLRFVDKLFEAVEEGRSSRHSKSSSDRSRKRELKEVFGDDSEISKESSGVKKRRIPRFEEVEEEPEVIPGPPSESPGMLTKLQIKQMMEAATRQIEERKKQLSFISPPTPQPKTPSSSQPERLPIGNTIQPSQAATFMNDAIEKARKAAELQARIQAQLALKPGLIGNANMVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEIELTHRMPTLKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEKLQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEHPAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLMPPPEPKVRISNLMRVLGTEAVQDPTKVEAHVRAQMAKRQKAHEEANAARKLTAEQRKVKKIKKLKEDISQGVHISVYRVRNLSNPAKKFKIEANAGQLYLTGVVVLHKDVNVVVVEGGPKAQKKFKRLMLHRIKWDEQTSNTKGDDDEESDEEAVKKTNKCVLVWEGTAKDRSFGEMKFKQCPTENMAREHFKKHGAEHYWDLALSESVLESTD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43395 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K17 | Ubiquitination | Uniprot | |
| C37 | S-Nitrosylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| R61 | Methylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| S161 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S164 | Phosphorylation | Uniprot | |
| T167 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T172 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| T186 | Phosphorylation | Uniprot | |
| K252 | Sumoylation | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| K294 | Methylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| K376 | Sumoylation | Uniprot | |
| Y411 | Phosphorylation | Uniprot | |
| T415 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| T494 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| T611 | Phosphorylation | Uniprot | |
| S619 | Phosphorylation | Uniprot |
Research Backgrounds
Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).
Ubiquitinated. Undergoes 'Lys-63'-linked polyubiquitination by PRPF19 and deubiquitination by USP4. 'Lys-63'-linked ubiquitination increases the affinity for PRPF8 and may regulate the assembly of the U4/U6-U5 tri-snRNP complex.
Nucleus. Nucleus speckle.
Highly expressed in retina, liver, kidney and blood. Detected at lower levels in heart and brain.
Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. Interacts directly with PRPF4. Part of a heteromeric complex containing PPIH, PRPF3 and PRPF4 that is stable in the absence of RNA. Interacts with SART3; the interaction is direct and recruits the deubiquitinase USP4 to PRPF3. Interacts with PRPF19. Interacts ('Lys-63'-linked polyubiquitinated) with PRPF8 (via the MPN (JAB/Mov34) domain); may stabilize the U4/U6-U5 tri-snRNP complex. Interacts with ERCC6.
Research Fields
· Genetic Information Processing > Transcription > Spliceosome.
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